解剖学杂志
解剖學雜誌
해부학잡지
CHINESE JOURNAL OF ANATOMY
2009年
5期
641-643,693,封2
,共5页
胡小令%袁铿%袁芳%吕诚%戴志芳%黄艳琴%胡银英%温蔚
鬍小令%袁鏗%袁芳%呂誠%戴誌芳%黃豔琴%鬍銀英%溫蔚
호소령%원갱%원방%려성%대지방%황염금%호은영%온위
神经干细胞%增殖%分化%神经调节蛋白-1%碱性成纤维细胞生长因子%大鼠
神經榦細胞%增殖%分化%神經調節蛋白-1%堿性成纖維細胞生長因子%大鼠
신경간세포%증식%분화%신경조절단백-1%감성성섬유세포생장인자%대서
neural stem cells%proliferation%differentiation%neuregulin-1%basic fibroblast growth factor%rat
目的:探讨神经调节蛋白-1(NRG-1)和碱性成纤维细胞生长因子(bFGF)对神经干细胞(NSCs)增殖和分化的影响.方法:从新生大鼠大脑组织分离NSCs,进行体外培养,分别用bFGF、 NRG-1和NRG-1加bFGF处理,观察各组神经球的形成情况;应用免疫细胞化学法鉴定NSCs及检测分化后神经元特异性烯醇化酶(NSE)、胶质纤维酸性蛋白(GFAP)、 2,3-环核苷酸磷酸二酯酶(CNP)的表达.结果:NRG+bFGF组和bFGF组形成的神经球数量和直径的差别不明显,但都明显多于和大于NRG组和对照组.免疫细胞化学显色结果显示,NRG+bFGF组、bFGF组中的神经球分化出的NSE、 GFAP、 CNP阳性细胞数量明显多于NRG组和对照组,尤其是NRG+bFGF组分化的NSE、CNP阳性细胞的突起较bFGF组更长和增多.结论:NRG-1与bFGF合用能促进NSCs的增殖和分化,促进分化的神经元、少突胶质细胞突起的生长.
目的:探討神經調節蛋白-1(NRG-1)和堿性成纖維細胞生長因子(bFGF)對神經榦細胞(NSCs)增殖和分化的影響.方法:從新生大鼠大腦組織分離NSCs,進行體外培養,分彆用bFGF、 NRG-1和NRG-1加bFGF處理,觀察各組神經毬的形成情況;應用免疫細胞化學法鑒定NSCs及檢測分化後神經元特異性烯醇化酶(NSE)、膠質纖維痠性蛋白(GFAP)、 2,3-環覈苷痠燐痠二酯酶(CNP)的錶達.結果:NRG+bFGF組和bFGF組形成的神經毬數量和直徑的差彆不明顯,但都明顯多于和大于NRG組和對照組.免疫細胞化學顯色結果顯示,NRG+bFGF組、bFGF組中的神經毬分化齣的NSE、 GFAP、 CNP暘性細胞數量明顯多于NRG組和對照組,尤其是NRG+bFGF組分化的NSE、CNP暘性細胞的突起較bFGF組更長和增多.結論:NRG-1與bFGF閤用能促進NSCs的增殖和分化,促進分化的神經元、少突膠質細胞突起的生長.
목적:탐토신경조절단백-1(NRG-1)화감성성섬유세포생장인자(bFGF)대신경간세포(NSCs)증식화분화적영향.방법:종신생대서대뇌조직분리NSCs,진행체외배양,분별용bFGF、 NRG-1화NRG-1가bFGF처리,관찰각조신경구적형성정황;응용면역세포화학법감정NSCs급검측분화후신경원특이성희순화매(NSE)、효질섬유산성단백(GFAP)、 2,3-배핵감산린산이지매(CNP)적표체.결과:NRG+bFGF조화bFGF조형성적신경구수량화직경적차별불명현,단도명현다우화대우NRG조화대조조.면역세포화학현색결과현시,NRG+bFGF조、bFGF조중적신경구분화출적NSE、 GFAP、 CNP양성세포수량명현다우NRG조화대조조,우기시NRG+bFGF조분화적NSE、CNP양성세포적돌기교bFGF조경장화증다.결론:NRG-1여bFGF합용능촉진NSCs적증식화분화,촉진분화적신경원、소돌효질세포돌기적생장.
Objective:To investigate the effects of neuregulin-1 (NRG-1) and basic fibroblast growth factor (bFGF) on proliferation and differentiation of cultared neural stem cells. Methods:The neural stem cells were isolated from postnatal rat telencephalon, and then treated with bFGF, NRG-1 and NRG-1 plus bFGF. The formation of neurospheres in the corresponding group was carefully monitored. Either the proliferation of neural stem cells or their differentiation into new neural and glial cell types could be identified by immunocytochemistry. Results:The number and diameter of the neurospheres showed no distinct difference between the NRG+bFGF group and bFGF group. However, more and larger neurospheres were observed in the NRG+bFGF group and bFGF group than those in the NRG group and control group. Furthermore, the immunocytochemical results showed that the neurospheres in the NRG+ bFGF group and bFGF group could differentiate into more cell types, such as antigen of neurons, astrocytes and oligodendrocytes, than those in the NRG group and control group. In particular, the protuberances of the NSE or CNP positive cells in the NRG+bFGF group were much longer and more than those in the bFGF groups. Conclusion:The experimental results indicate that the combination of NRG-1 and bFGF can promote the proliferation and differentiation of the neural stem cells, which are isolated from postnatal rat telencephalon, as well as the growth of the protuberances of neurons and oligodendrocytes.