基础医学与临床
基礎醫學與臨床
기출의학여림상
BASIC MEDICAL SCIENCES AND CLINICS
2010年
1期
37-41
,共5页
NDRG2%肿瘤%基因转染%腺病毒
NDRG2%腫瘤%基因轉染%腺病毒
NDRG2%종류%기인전염%선병독
NDRG2%tumor%gene transfection%adenovirus
目的 观察NDRG2对于乳腺癌细胞系增殖的影响.方法 用重组NDRG2腺病毒转染低表达NDRG2的人乳腺癌细胞系Bcap37,用Western blot检测NDRG2的表达;用MTT、流式细胞仪检测细胞增殖能力.结果 NDRG2的表达对Bcap37细胞生长具有抑制作用;Bcap37细胞病毒转染组出现G_1期阻滞(G_1期捕获),与未转染的Bcap37细胞(1.0%)和空载体重组腺病毒感染的Bcap37细胞(2.0%)相比,重组NDRG2腺病毒感染的Bcap37细胞中凋亡细胞明显增多,感染48 h后达12.0%,感染72 h后达21.5%.结论 在乳腺癌细胞系Bcap37中,过表达NDRG2可有效抑制细胞增殖并导致细胞周期的阻滞和诱导细胞凋亡.
目的 觀察NDRG2對于乳腺癌細胞繫增殖的影響.方法 用重組NDRG2腺病毒轉染低錶達NDRG2的人乳腺癌細胞繫Bcap37,用Western blot檢測NDRG2的錶達;用MTT、流式細胞儀檢測細胞增殖能力.結果 NDRG2的錶達對Bcap37細胞生長具有抑製作用;Bcap37細胞病毒轉染組齣現G_1期阻滯(G_1期捕穫),與未轉染的Bcap37細胞(1.0%)和空載體重組腺病毒感染的Bcap37細胞(2.0%)相比,重組NDRG2腺病毒感染的Bcap37細胞中凋亡細胞明顯增多,感染48 h後達12.0%,感染72 h後達21.5%.結論 在乳腺癌細胞繫Bcap37中,過錶達NDRG2可有效抑製細胞增殖併導緻細胞週期的阻滯和誘導細胞凋亡.
목적 관찰NDRG2대우유선암세포계증식적영향.방법 용중조NDRG2선병독전염저표체NDRG2적인유선암세포계Bcap37,용Western blot검측NDRG2적표체;용MTT、류식세포의검측세포증식능력.결과 NDRG2적표체대Bcap37세포생장구유억제작용;Bcap37세포병독전염조출현G_1기조체(G_1기포획),여미전염적Bcap37세포(1.0%)화공재체중조선병독감염적Bcap37세포(2.0%)상비,중조NDRG2선병독감염적Bcap37세포중조망세포명현증다,감염48 h후체12.0%,감염72 h후체21.5%.결론 재유선암세포계Bcap37중,과표체NDRG2가유효억제세포증식병도치세포주기적조체화유도세포조망.
Objective To explore the effect of gene NDRG2 (N-myc downstream regulated gene 2) transfection on the proliferation and apoptosis of breast cancer cell. Methods Bcap37 cell line expressing low level product encoded by NDRG2 gene is transfected with recombinating adenovirus expressing high level NDRG2 gene product to improve NDRG2 gene expression level. NDRG2 expression was detected by Western blot and the proliferation activity of the cell lines was detected by MTT and flow cytometry. Results High level expression of NDRG2 inhibits cells growth and results in G_1 phase arrest in Bcap37 cell line. Compared with non-transfected Bcap37 cells and Bcap37 cells transfected by empty vector, apoptosis cells obviously increase in Bcap37 cell line transfected by recombinating adenovirus expressing high level NDRG2 gene, 12% after 48 hours and 21. 5% after 72 hours. Conclusion Overexpres-sion of NDRG2 in Bcap37 cells effectively inhibites cell proliferation and induces cell cycle arrest and apoptosis.
