中国组织化学与细胞化学杂志
中國組織化學與細胞化學雜誌
중국조직화학여세포화학잡지
CHINESE JOURNAL OF HISTOCHEMISY AND CYTOCHEMISY
2010年
1期
32-37
,共6页
牛慧彦%王佳贺%徐灿%李慧%何平
牛慧彥%王佳賀%徐燦%李慧%何平
우혜언%왕가하%서찬%리혜%하평
鸟嘌呤核苷酸解离抑制因子2%肺鳞癌%肺腺癌%细胞系%转移
鳥嘌呤覈苷痠解離抑製因子2%肺鱗癌%肺腺癌%細胞繫%轉移
조표령핵감산해리억제인자2%폐린암%폐선암%세포계%전이
RhoGDI2%Squamous carcinoma%Adenocarcinoma%Cell line%Metastasis
目的 本研究探讨RhoGDI2在肺鳞癌和腺癌组织及肺癌细胞系中的作用及其临床意义.方法 采用免疫组织化学和RT-PCR方法检测112例肺癌组织标本中RhoGDI2蛋白和20例新鲜肺癌组织中RhoGDI2 mRNA的表达,结合肺癌的临床病理特点进行分析.同时应用Western blot和RT-PCR方法检测肺癌细胞系中RhoGDI2蛋白和mRNA水平的表达情况.结果 肺鳞癌和腺癌组织中RhoGDI2的表达与组织分级有关,随着分化程度的降低,RhoGDI2蛋白表达降低(P<0.01);与TNM分期有关,随着分期级别的升高,RhoGDI2蛋白表达降低(P<0.01);与是否淋巴结转移有关,存在淋巴结转移的标本,RhoGDI2蛋白表达降低(P<0.01).RhoGDI2蛋白的表达与组织类型、患者的年龄和性别无关.在选取的肺癌细胞系A549、95D、SPC-A-1和NCI-H446中,无论mRNA水平还是蛋白水平RhoGDI2都有表达,但表达水平不尽相同,在A549、NCI-H446细胞系中表达较低,在SPC-A-1、95D细胞系中表达相对较高.结论 RhoGDI2与肺癌的发生发展和转移过程有关,进一步研究RhoGDI2与其作用因子之间的相互作用,将有助于进一步揭示肺癌发生发展及转移过程.
目的 本研究探討RhoGDI2在肺鱗癌和腺癌組織及肺癌細胞繫中的作用及其臨床意義.方法 採用免疫組織化學和RT-PCR方法檢測112例肺癌組織標本中RhoGDI2蛋白和20例新鮮肺癌組織中RhoGDI2 mRNA的錶達,結閤肺癌的臨床病理特點進行分析.同時應用Western blot和RT-PCR方法檢測肺癌細胞繫中RhoGDI2蛋白和mRNA水平的錶達情況.結果 肺鱗癌和腺癌組織中RhoGDI2的錶達與組織分級有關,隨著分化程度的降低,RhoGDI2蛋白錶達降低(P<0.01);與TNM分期有關,隨著分期級彆的升高,RhoGDI2蛋白錶達降低(P<0.01);與是否淋巴結轉移有關,存在淋巴結轉移的標本,RhoGDI2蛋白錶達降低(P<0.01).RhoGDI2蛋白的錶達與組織類型、患者的年齡和性彆無關.在選取的肺癌細胞繫A549、95D、SPC-A-1和NCI-H446中,無論mRNA水平還是蛋白水平RhoGDI2都有錶達,但錶達水平不儘相同,在A549、NCI-H446細胞繫中錶達較低,在SPC-A-1、95D細胞繫中錶達相對較高.結論 RhoGDI2與肺癌的髮生髮展和轉移過程有關,進一步研究RhoGDI2與其作用因子之間的相互作用,將有助于進一步揭示肺癌髮生髮展及轉移過程.
목적 본연구탐토RhoGDI2재폐린암화선암조직급폐암세포계중적작용급기림상의의.방법 채용면역조직화학화RT-PCR방법검측112례폐암조직표본중RhoGDI2단백화20례신선폐암조직중RhoGDI2 mRNA적표체,결합폐암적림상병리특점진행분석.동시응용Western blot화RT-PCR방법검측폐암세포계중RhoGDI2단백화mRNA수평적표체정황.결과 폐린암화선암조직중RhoGDI2적표체여조직분급유관,수착분화정도적강저,RhoGDI2단백표체강저(P<0.01);여TNM분기유관,수착분기급별적승고,RhoGDI2단백표체강저(P<0.01);여시부림파결전이유관,존재림파결전이적표본,RhoGDI2단백표체강저(P<0.01).RhoGDI2단백적표체여조직류형、환자적년령화성별무관.재선취적폐암세포계A549、95D、SPC-A-1화NCI-H446중,무론mRNA수평환시단백수평RhoGDI2도유표체,단표체수평불진상동,재A549、NCI-H446세포계중표체교저,재SPC-A-1、95D세포계중표체상대교고.결론 RhoGDI2여폐암적발생발전화전이과정유관,진일보연구RhoGDI2여기작용인자지간적상호작용,장유조우진일보게시폐암발생발전급전이과정.
Objective To investigate the expression of RhoGDI2 in lung squamous carcinoma, adenocarcinoma and lung cancer cell lines, and to analyze its clinical significance.Methods The expression of RhoGDI2 protein was detected by immunohistochemistry in 112 lung cancer cases and analyzed with their clinicopathologic characteristics; RT-PCR method was used to detect RhoGDI2 mRNA in 20 fresh lung cancer tissues. Meanwhile, in the lung cancer cell lines, the RhoGDI2 protein and mRNA expressions and location were detected by Western blot and RT-PCR.Results In lung cancer tissues, the expression of RhoGDI2 was correlated with the histologic grading, staging and lymph node metastasis. The RhoGDI2 down-regulation was significantly correlated with the poor differentiation (P<0.01), late TNM stage (P<0.01) and lymph node metastasis (P<0.01), but not correlated with age, gender and histological type. Also, RhoGDI2 protein and mRNA were differently expressed in A549, SPC-A-1, 95D and NCI-H446 lung cancer cell lines. Conclusion RhoGDI2 is related with the oncogenesis and metastasis of lung cancer. The further study of RhoGDI2 and its interactional factors and signals may help us understand the oncogenesis and metastasis of lung cancer.
