80 MeV/u C-12离子诱变选育PHB高产菌株
80 MeV/u C-12리자유변선육PHB고산균주
A strain of high PHB output by mutagenesis of 80 MeV/u C-12 ions
  • 万方数据
    核技术 핵기술
    NUCLEAR TECHNIQUES
    2010年 4期 284-288 ,共5页

    薛林贵%赵旭%常思静%张红%武振华%景春娥

    설림귀%조욱%상사정%장홍%무진화%경춘아

    聚β-羟基丁酸%重离子%诱变%高产菌 聚β-羥基丁痠%重離子%誘變%高產菌
    취β-간기정산%중리자%유변%고산균
    Poly-β-hydroxybutyrate%Heavy ion%Nutagenesis%High yielding strain
    用80 MeV/u 12C6+离子对一株积累聚β-羟基丁酸酯(PHB)的芽孢杆菌(Bacillus P-9)进行诱变选育,最终获得PHB高产株菌G15,其PHB产量达2.93 g/L,与出发菌株相比较,PHB积累量提高了1.5倍.研究结果表明,本试验辐照最佳剂量为15Gy.重离子辐照诱变育种效果显著,具有广阔的发展应用前景.
    용80 MeV/u 12C6+리자대일주적루취β-간기정산지(PHB)적아포간균(Bacillus P-9)진행유변선육,최종획득PHB고산주균G15,기PHB산량체2.93 g/L,여출발균주상비교,PHB적루량제고료1.5배.연구결과표명,본시험복조최가제량위15Gy.중리자복조유변육충효과현저,구유엄활적발전응용전경.
    The PHB-producing strain of Bacillus P-9 was irradiated by 80 MeV/u 12C6+ ion beams to increase PHB production. The results indicated that the best radiation dose is 15 Gy. One strain mutagenized the 12C6+ ion beams,called G15,has the highest PHB production yield,showing final PHB concentration of 2.93 g/L,1.5 times higher than that of original strain. The breeding effect of heavy-ion irradiation mutation is remarkable,and has a broad application prospect.
    원문보기 원문다운로드 사이트로이동
저자의 최근 논문