CAJ | 학술논문

采用批次培养方法,在光照强度60、110 μmol/m~2·s下分别设置了7个不同的氮、磷浓度(N:0-3500 μg/L,P:15-775 μg/L),研究两株布朗葡萄藻(Botryococcus braunii)对氮、磷胁迫的敏感性差异,筛选高营养利用效率的优良藻株.结果表明:两株藻对氮磷营养胁迫的耐受性存在差异,B.braunii 764株对氮胁迫具有较高耐受性,而B.braunii 765株对磷胁迫具有较高耐受性.光照强度110 μmol/m~2·s,不同氮浓度下B.braunii 764株其平均生长速率均显著高于其他各处理组;不同磷浓度下B.braunii 765株其平均生长速率显著高于B.braunii764株.在试验设定的光照强度条件下.适当增加光照强度能够显著降低氮胁迫对布朗葡萄藻生长的抑制效应.在光照强度110 μmol/m~2·s下,氮浓度3500μg/L时两株布朗葡萄藻平均生长速率与在正常Chu-10培养基条件下无显著差异.磷浓度775μg/L时两株布朗葡萄藻的平均生长速率均显著低于正常Chu-10培养基条件,增加光照强度对磷胁迫下藻细胞的生长无显著作用.两株布朗葡萄藻在第2天时磷吸收与初始磷浓度呈正相关关系,氮吸收在3500 μg/L时出现饱和现象.布朗葡萄藻的生长更容易受到培养基中磷营养胁迫的影响.
채용비차배양방법,재광조강도60、110 μmol/m~2·s하분별설치료7개불동적담、린농도(N:0-3500 μg/L,P:15-775 μg/L),연구량주포랑포도조(Botryococcus braunii)대담、린협박적민감성차이,사선고영양이용효솔적우량조주.결과표명:량주조대담린영양협박적내수성존재차이,B.braunii 764주대담협박구유교고내수성,이B.braunii 765주대린협박구유교고내수성.광조강도110 μmol/m~2·s,불동담농도하B.braunii 764주기평균생장속솔균현저고우기타각처리조;불동린농도하B.braunii 765주기평균생장속솔현저고우B.braunii764주.재시험설정적광조강도조건하.괄당증가광조강도능구현저강저담협박대포랑포도조생장적억제효응.재광조강도110 μmol/m~2·s하,담농도3500μg/L시량주포랑포도조평균생장속솔여재정상Chu-10배양기조건하무현저차이.린농도775μg/L시량주포랑포도조적평균생장속솔균현저저우정상Chu-10배양기조건,증가광조강도대린협박하조세포적생장무현저작용.량주포랑포도조재제2천시린흡수여초시린농도정정상관관계,담흡수재3500 μg/L시출현포화현상.포랑포도조적생장경용역수도배양기중린영양협박적영향.
The growth of two Botryococcus braunii strains were studied under seven nitrogen and phosphorus stress levels (N: 0-3500/μg/L, P: 15-775μg/L) in batch culture at two light intensities of 60 and 110 μmol/m~2.s. Results showed that the tolerance of B. braunii to N and P stress was different between strains, which was further affected by light intensity. B. braunii 764 grew better under N stress, while B. braunii 765 grew better under P stress. The average growth rates of B. braunii 764 with all N stress treatments were significantly higher at the light intensity of 110 μmol/m~2.s than any other treatments. There was no significant difference among the average growth rates of B. braunii 764 and 765 at 60 μmol/m~2.s and B. braunii 765 at 110 μmol/m~2.s treated with N stress. The average growth rates of B. braunii 764 at 110 μmol/m~2.s were significantly lower than other P stress treatments. There was no significant difference among the average growth rate S or B. braunii 764 and 765 at 60μmol/m~2.s and B. braunii 765 at 110 μmol/m~2.s treated with P stress. Inhibitory effect of N stress on the growth of B. braunii strains was alleviated by the increase of light in-tensity from 60 to 110/μmol/m~2.s. The growth rates of two B. braunii strains under 3500 μg/L N treatment showed no notable difference compared with that under normal Chu-10 medium. The uptake rates of N and P exhibited linear de-pendence with the initial levels at the 2~(nd) day. N uptake rate reached a saturation point at 3500 μg/L N at the 2~(nd) day.