CAJ | 학술논문

在5L发酵罐下开展毕赤酵母流加培养表达pIFN-α研究.通过多批次研究,结果表明:诱导阶段,RQ稳定情况可以作为反映底物甲醇浓度水平是否合适以及pIFN-α活性高低的监控指标.当甲醇浓度在8～12g/L水平时,RQ在整个诱导阶段能稳定在0.4～0.5之间某个特定值上,同时pIFN-α最高活性都能维持在10~6IU/mL水平上;而当甲醇浓度在0～5g/L或15～20g/L范围内时,RQ很不稳定,分别在0.15～0.4和0.4～0.8区间上下振动,相应的pIFN-α最高活性也只在10~3IU/mL和10~4IU/mL水平上.
재5L발효관하개전필적효모류가배양표체pIFN-α연구.통과다비차연구,결과표명:유도계단,RQ은정정황가이작위반영저물갑순농도수평시부합괄이급pIFN-α활성고저적감공지표.당갑순농도재8～12g/L수평시,RQ재정개유도계단능은정재0.4～0.5지간모개특정치상,동시pIFN-α최고활성도능유지재10~6IU/mL수평상;이당갑순농도재0～5g/L혹15～20g/L범위내시,RQ흔불은정,분별재0.15～0.4화0.4～0.8구간상하진동,상응적pIFN-α최고활성야지재10~3IU/mL화10~4IU/mL수평상.
Porcine interferon-α (pIFN-α) expression under high density recombinant Pichia pastoris cultivation was conducted in a 5L bench-acaled bioreactor.The results indicated that the stability of respiratory quotient (RQ) could reflect the suitability of methanol concentration for Pichia pastoris induction expression and the pIFN-α activity level indirectly.Maintained with a moderate induction methanol concentration of 8 ～12g/L,RQ could keep constant at a certain value between 0.4 and 0.5 in the whole induction stage.Meanwhile,the highest pIFN-α activity could keep above 10~6 IU/mL.When the methanol concentration was maintained at a lower level of 0 ～ 5g/L or a higher level of 15 ～ 20g/L,the RQ was unstable and the highest pIFN-α activities were at the level of 10~3IU/mL and 10~4 IU/mL,respectively.