CAJ | 학술논문

以螺旋木霉(Trichoderma Spirale XX)、小克银汉霉(Cunninghamella phaeospora MK)和卵形孢球托霉(Gongronella butleri XT)3种丝状真菌为材料,采用改进的CTAB法提取基因组DNA.方法改进后无需液氮、聚乙烯砒略烷酮(PVP)和NaAc等试剂,过程简洁,且所需菌体量少,提取的DNA纯度较好,适用于一次微量提取多个样品的基因组DNA.此方法得到的基因组DNA可用于PCR扩增.
이라선목매(Trichoderma Spirale XX)、소극은한매(Cunninghamella phaeospora MK)화란형포구탁매(Gongronella butleri XT)3충사상진균위재료,채용개진적CTAB법제취기인조DNA.방법개진후무수액담、취을희비략완동(PVP)화NaAc등시제,과정간길,차소수균체량소,제취적DNA순도교호,괄용우일차미량제취다개양품적기인조DNA.차방법득도적기인조DNA가용우PCR확증.
Genomic DNA of the three filamentous fungi Trichoderma Spirale XX, Cunninghamella phaeospora MK and Gongronella butleri XT were extracted with a modified Cetyltrimethyl Ammonium Bromide (CTAB) method. The improued method does not require liquid nitrogen, PVP and NaAc etc. The process was simple and efficient. The result indicated that higher purity of genomic DNA was obtained with less amount of mycelium by the method which was suitable to treat many samples at one time, and genomic DNA for basic molecular experiments such as PCR.