中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2009年
11期
742-744
,共3页
魏义花%李海英%张玉杰%杨磊%王秀敏%屈明芬%冯爱平%沈关心%周淑华
魏義花%李海英%張玉傑%楊磊%王秀敏%屈明芬%馮愛平%瀋關心%週淑華
위의화%리해영%장옥걸%양뢰%왕수민%굴명분%풍애평%침관심%주숙화
紫癜%过敏性%白细胞%抗原%CD80%PD-1
紫癜%過敏性%白細胞%抗原%CD80%PD-1
자전%과민성%백세포%항원%CD80%PD-1
Pnrpura%Sch(o)nlein-Henoch%Lcukocytes%Antigens%CD80%PD-1
目的 探讨过敏性紫癜患者外周血白细胞B7-H1和PD-1分子的表达及其意义.方法 应用荧光抗体染色技术经流式细胞仪检测36例过敏性紫癜患者和24例正常人对照外周血白细胞表面的B7-H1及PD-1分子的表达水平,并比较两者在过敏性紫癜患者与正常人对照之间以及在伴紫癜性肾炎组患者和非紫癜性肾炎组患者之间的表达水平差异.结果 过敏性紫癜患者组B7-H1及PD-1在单核细胞上的阳性表达率分别为24.43%±25.79%,0.84%±1.96%;正常人对照组分别为7.69%±8.31%、2.28%±1.95%.过敏性紫癜患者组外周血单核细胞B7-H1表达水平显著高于正常人对照组(t=3.62,P＜0.01),而PD-1表达水平则显著低于jE常人对照组(t=2.78,P＜0.01).患者组和对照组淋巴细胞表面B7-H1及PD-1的表达差异无统计学意义,P值均＞0.05.过敏性紫癜患者中伴肾炎组患者B7-H1在单核细胞及淋巴细胞上的15H性表达率分别为44.81%±12.36%,8.78%±2.10%;无肾炎组分别为17.63%±25.63%,5.65%±3.96%;伴肾炎患者单核细胞及淋巴细胞的B7-H1的表达水平均显著高于无肾炎患者,t=3.05,2.25,P值＜0.01或0.05.结论 过敏性紫癜患者外周血单核细胞表面B7-H1表达升高,而PD-1表达下降,B7-H1与PD-1可能在过敏性紫癜发病机制中发挥一定作用.
目的 探討過敏性紫癜患者外週血白細胞B7-H1和PD-1分子的錶達及其意義.方法 應用熒光抗體染色技術經流式細胞儀檢測36例過敏性紫癜患者和24例正常人對照外週血白細胞錶麵的B7-H1及PD-1分子的錶達水平,併比較兩者在過敏性紫癜患者與正常人對照之間以及在伴紫癜性腎炎組患者和非紫癜性腎炎組患者之間的錶達水平差異.結果 過敏性紫癜患者組B7-H1及PD-1在單覈細胞上的暘性錶達率分彆為24.43%±25.79%,0.84%±1.96%;正常人對照組分彆為7.69%±8.31%、2.28%±1.95%.過敏性紫癜患者組外週血單覈細胞B7-H1錶達水平顯著高于正常人對照組(t=3.62,P＜0.01),而PD-1錶達水平則顯著低于jE常人對照組(t=2.78,P＜0.01).患者組和對照組淋巴細胞錶麵B7-H1及PD-1的錶達差異無統計學意義,P值均＞0.05.過敏性紫癜患者中伴腎炎組患者B7-H1在單覈細胞及淋巴細胞上的15H性錶達率分彆為44.81%±12.36%,8.78%±2.10%;無腎炎組分彆為17.63%±25.63%,5.65%±3.96%;伴腎炎患者單覈細胞及淋巴細胞的B7-H1的錶達水平均顯著高于無腎炎患者,t=3.05,2.25,P值＜0.01或0.05.結論 過敏性紫癜患者外週血單覈細胞錶麵B7-H1錶達升高,而PD-1錶達下降,B7-H1與PD-1可能在過敏性紫癜髮病機製中髮揮一定作用.
목적 탐토과민성자전환자외주혈백세포B7-H1화PD-1분자적표체급기의의.방법 응용형광항체염색기술경류식세포의검측36례과민성자전환자화24례정상인대조외주혈백세포표면적B7-H1급PD-1분자적표체수평,병비교량자재과민성자전환자여정상인대조지간이급재반자전성신염조환자화비자전성신염조환자지간적표체수평차이.결과 과민성자전환자조B7-H1급PD-1재단핵세포상적양성표체솔분별위24.43%±25.79%,0.84%±1.96%;정상인대조조분별위7.69%±8.31%、2.28%±1.95%.과민성자전환자조외주혈단핵세포B7-H1표체수평현저고우정상인대조조(t=3.62,P＜0.01),이PD-1표체수평칙현저저우jE상인대조조(t=2.78,P＜0.01).환자조화대조조림파세포표면B7-H1급PD-1적표체차이무통계학의의,P치균＞0.05.과민성자전환자중반신염조환자B7-H1재단핵세포급림파세포상적15H성표체솔분별위44.81%±12.36%,8.78%±2.10%;무신염조분별위17.63%±25.63%,5.65%±3.96%;반신염환자단핵세포급림파세포적B7-H1적표체수평균현저고우무신염환자,t=3.05,2.25,P치＜0.01혹0.05.결론 과민성자전환자외주혈단핵세포표면B7-H1표체승고,이PD-1표체하강,B7-H1여PD-1가능재과민성자전발병궤제중발휘일정작용.
Objective To investigate the expressions of B7-H1 and PD-1 on peripheral leukocytes from patients with Henoch-Schtmlein purpura(HSP)and their significance.Methods Peripheral leukocytes were obtained from 36 patients with HSP(HSP group)and 24 healthy human controls(control group).Immunofluorescent staining and flow cytometry were used to measure the expressions of B7-H1 and PD-1 on peripheral leukocytes.The expression of both two molecules was compared between the HSP group and control group as well as between patients with Henoch-Sch(o)nlein purpura nephritis(HSPN)(n=9)and those without(n=27).SPSS 12.0 for Windows was used for data analysis.Results The expression rate of B7-H1 on monocytes significantly increased(24.43%±25.79%vs 7.69%±8.31%,t=3.62,P＜0.011),while that of PD-1 decreased(0.84%±1.96%vs 2.28%±1.95%,t=2.78,P＜0.01)in HSP group compared with those in the control group.No significant difference was revealed in the expression of B7-H1 or PD-1 on lymphocytes between HSP group and control group(both P＞0.05).There was a significant increase in the expression of B7-H1 on monocytes(44.81%±12.36%vs 17.63%±25.63%,t=3.05,P＜0.01)and lymphocytes(8.78%±2.10%vs 5.65%±3.96%,t=2.25,P＜0.05)in patients with HSPN compared with those without.Conclusion There is a high expression of B7-H1.but low expression of PD-1 on peripheral blood monocytes from patients with HSP.suggesting that B7-H1 and PD-1 may play a certain role in the Dathogenesis of HSP.
