中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2012年
2期
215-219
,共5页
谢佳新%殷建华%张琪%蒲蕊%张玉伟%鹿文英%曹广文
謝佳新%慇建華%張琪%蒲蕊%張玉偉%鹿文英%曹廣文
사가신%은건화%장기%포예%장옥위%록문영%조엄문
肝细胞癌%JAK/STAT信号通路%单核苷酸多态性
肝細胞癌%JAK/STAT信號通路%單覈苷痠多態性
간세포암%JAK/STAT신호통로%단핵감산다태성
Hepatocellular carcinoma%JAK/STAT signaling pathway%Single nucleotide polymorphism
目的 探讨宿主JAK/STAT信号通路中关键分子基因多态性与肝细胞癌(HCC)易感性的关系.方法 采用病例对照研究,用质谱法对367例诊断明确的HBsAg阳性HCC患者(HCC组)和性别、年龄匹配的367例对照的IL-6(rs1800796,-572C＞G)、STAT3 (rs744166,+26312T＞C; rs3816769,+42240T＞C; rs6503695,+40980T＞C)、EGFR(rs11543848,+142530A＞G)、mTOR(rs7211818,+170278A＞G;rs9674559,+196983A＞G;rs11653499,+65678G＞A)进行多态性检测.单因素分析确定各位点多态性比值比(OR)和95％可信区间(CI).结果 IL-6、STAT3、EGFR、mTOR的8个多态性位点各基因型在HCC组和对照组中分布的差异无统计学意义(P＞0.05).按性别分层后,在女性中,与TT基因型相比,携带STAT3 +26312CC、+42240CC、+40980CC基因型个体患HCC的危险性降低(OR=0.192,95％CI:0.047 ～ 0.784；OR=0.180,95％CI:0.045 ～ 0.725；OR=0.198,95％CI:0.049 ～ 0.806).与AA基因型相比,EGFR+142530(AG+GG)基因型降低女性患HCC的风险(OR=0.422,95％CI:0.179～ 0.994).结论 IL-6 (rs 1800796)、mTOR(rs7211818,rs9674559,rs11653499)多态性与HCC易感性无关；女性携带STAT3 (rs744166,rs3816769,rs6503695) CC基因型及EGFR(rs11543848) (AG+ GG)的个体患HCC的风险降低,但还需更大样本验证.
目的 探討宿主JAK/STAT信號通路中關鍵分子基因多態性與肝細胞癌(HCC)易感性的關繫.方法 採用病例對照研究,用質譜法對367例診斷明確的HBsAg暘性HCC患者(HCC組)和性彆、年齡匹配的367例對照的IL-6(rs1800796,-572C＞G)、STAT3 (rs744166,+26312T＞C; rs3816769,+42240T＞C; rs6503695,+40980T＞C)、EGFR(rs11543848,+142530A＞G)、mTOR(rs7211818,+170278A＞G;rs9674559,+196983A＞G;rs11653499,+65678G＞A)進行多態性檢測.單因素分析確定各位點多態性比值比(OR)和95％可信區間(CI).結果 IL-6、STAT3、EGFR、mTOR的8箇多態性位點各基因型在HCC組和對照組中分佈的差異無統計學意義(P＞0.05).按性彆分層後,在女性中,與TT基因型相比,攜帶STAT3 +26312CC、+42240CC、+40980CC基因型箇體患HCC的危險性降低(OR=0.192,95％CI:0.047 ～ 0.784；OR=0.180,95％CI:0.045 ～ 0.725；OR=0.198,95％CI:0.049 ～ 0.806).與AA基因型相比,EGFR+142530(AG+GG)基因型降低女性患HCC的風險(OR=0.422,95％CI:0.179～ 0.994).結論 IL-6 (rs 1800796)、mTOR(rs7211818,rs9674559,rs11653499)多態性與HCC易感性無關；女性攜帶STAT3 (rs744166,rs3816769,rs6503695) CC基因型及EGFR(rs11543848) (AG+ GG)的箇體患HCC的風險降低,但還需更大樣本驗證.
목적 탐토숙주JAK/STAT신호통로중관건분자기인다태성여간세포암(HCC)역감성적관계.방법 채용병례대조연구,용질보법대367례진단명학적HBsAg양성HCC환자(HCC조)화성별、년령필배적367례대조적IL-6(rs1800796,-572C＞G)、STAT3 (rs744166,+26312T＞C; rs3816769,+42240T＞C; rs6503695,+40980T＞C)、EGFR(rs11543848,+142530A＞G)、mTOR(rs7211818,+170278A＞G;rs9674559,+196983A＞G;rs11653499,+65678G＞A)진행다태성검측.단인소분석학정각위점다태성비치비(OR)화95％가신구간(CI).결과 IL-6、STAT3、EGFR、mTOR적8개다태성위점각기인형재HCC조화대조조중분포적차이무통계학의의(P＞0.05).안성별분층후,재녀성중,여TT기인형상비,휴대STAT3 +26312CC、+42240CC、+40980CC기인형개체환HCC적위험성강저(OR=0.192,95％CI:0.047 ～ 0.784；OR=0.180,95％CI:0.045 ～ 0.725；OR=0.198,95％CI:0.049 ～ 0.806).여AA기인형상비,EGFR+142530(AG+GG)기인형강저녀성환HCC적풍험(OR=0.422,95％CI:0.179～ 0.994).결론 IL-6 (rs 1800796)、mTOR(rs7211818,rs9674559,rs11653499)다태성여HCC역감성무관；녀성휴대STAT3 (rs744166,rs3816769,rs6503695) CC기인형급EGFR(rs11543848) (AG+ GG)적개체환HCC적풍험강저,단환수경대양본험증.
Objective To elucidate the association of genetic polymorphisms of key molecules in JAK/STAT signaling pathway with susceptibility of hepatocellular carcinoma (HCC).Methods A total of 367 HCC patients and 367 healthy controls were recruited in this sex- and age-matched case-control study.Genetic polymorphisms of IL-6 (rs1800796,-572C＞G),STAT3 (rs744166,+ 26312T＞C; rs3816769,+ 42240T＞C; rs6503695,+ 40980T＞C),EGFR (rs11543848,+ 142530A＞G),and mTOR (rs7211818,+ 170278A＞G; rs9674559,+ 196983A＞G; rs11653499,+65678G＞A) were genotyped using a mass spectrometry method.Odds ratio (OR) and 95％ confidence interval (CI) were calculated.Results Genotype frequency of the 8 polymorphisms of IL-6,STAT3,EGFR,and mTOR were not significantly different between the patients with HCC and the controls.When stratified by sex,the female subjects who carried STAT3 +26312CC,+ 42240CC,or + 40980CC had a decreased risk of HCC when compared to those who carried TT allele (OR=0.192,95％CI:0.047-0.784; OR=0.180,95％CI:0.045-0.725;OR=0.198,95％ CI:0.049-0.806,respectively).When compared with AA genotype on the site of EGFR + 142530,the (AG+ GG) genotype reduced the risk of HCC in women (OR=0.422,95％CI:0.179-0.994).Conclusion The polymorphisms of IL-6 (rs1800796) and mTOR (rs7211818,rs9674559,and rs11653499) were not associated with the HCC susceptibility.Those carrying CC allele in three loci (rs744166,rs3816769,and rs6503695) of STAT3 and (AG + GG) in rs11543848 of EGFR had a decreased risk of HCC in women.However,these results need to be validated using larger sample size.
