中国实用医刊
中國實用醫刊
중국실용의간
CENTRAL PLAINS MEDICAL JOURNAL
2010年
13期
23-24,27
,共3页
胃癌%幽门螺杆菌%细胞增殖核抗原%端粒酶逆转录酶
胃癌%幽門螺桿菌%細胞增殖覈抗原%耑粒酶逆轉錄酶
위암%유문라간균%세포증식핵항원%단립매역전록매
Gastric carcinoma%Helicobacter pylori%Cell proliferation of nuclear antigen%Telomerase reverse transcriptase
目的 探讨胃癌及癌前病变组织中端粒酶逆转录酶(TERT)和细胞增殖核抗原(PCNA)的表达及其临床意义,并分析幽门螺杆菌(Hp)感染与端粒酶逆转录酶(TERT)和细胞增殖核抗原表达的关系.方法 采用Warthin-Starry(W-S)法、原位杂交法及免疫组化技术检测127例经病理证实的不同胃黏膜病变中Hp感染、TERT mRNA、PCNA的表达.结果 胃癌组PCNA和TERT mRNA阳性率均分别高于慢性萎缩性胃炎、肠上皮化生、异型增生(P＜0.05);Hp阳性组PCNA和TERT表达均高于Hp阴性组(P＜0.01).结论 Hp可通过刺激胃黏膜细胞过度增殖,同时诱导端粒酶活化和PCNA异常表达,从而启动和加速胃癌的发生发展过程.PCNA、TERT的过度表达与胃癌的发生有关,可作为筛选胃癌高危癌前病变的标志物.
目的 探討胃癌及癌前病變組織中耑粒酶逆轉錄酶(TERT)和細胞增殖覈抗原(PCNA)的錶達及其臨床意義,併分析幽門螺桿菌(Hp)感染與耑粒酶逆轉錄酶(TERT)和細胞增殖覈抗原錶達的關繫.方法 採用Warthin-Starry(W-S)法、原位雜交法及免疫組化技術檢測127例經病理證實的不同胃黏膜病變中Hp感染、TERT mRNA、PCNA的錶達.結果 胃癌組PCNA和TERT mRNA暘性率均分彆高于慢性萎縮性胃炎、腸上皮化生、異型增生(P＜0.05);Hp暘性組PCNA和TERT錶達均高于Hp陰性組(P＜0.01).結論 Hp可通過刺激胃黏膜細胞過度增殖,同時誘導耑粒酶活化和PCNA異常錶達,從而啟動和加速胃癌的髮生髮展過程.PCNA、TERT的過度錶達與胃癌的髮生有關,可作為篩選胃癌高危癌前病變的標誌物.
목적 탐토위암급암전병변조직중단립매역전록매(TERT)화세포증식핵항원(PCNA)적표체급기림상의의,병분석유문라간균(Hp)감염여단립매역전록매(TERT)화세포증식핵항원표체적관계.방법 채용Warthin-Starry(W-S)법、원위잡교법급면역조화기술검측127례경병리증실적불동위점막병변중Hp감염、TERT mRNA、PCNA적표체.결과 위암조PCNA화TERT mRNA양성솔균분별고우만성위축성위염、장상피화생、이형증생(P＜0.05);Hp양성조PCNA화TERT표체균고우Hp음성조(P＜0.01).결론 Hp가통과자격위점막세포과도증식,동시유도단립매활화화PCNA이상표체,종이계동화가속위암적발생발전과정.PCNA、TERT적과도표체여위암적발생유관,가작위사선위암고위암전병변적표지물.
Objective To investigate the expressions of cell proliferation of nuclear antigen (PCNA) and telomerase reverse transcriptase (TERT) mRNA and their clinical significance ingastri precance-rous lesion and carcinoma.To analyse the relationship between Helicobacter pylori infection and expressions of TERT and PCNA. Methods Expressions of Hp,TERT mRNA and proliferating cell nuclear antigen in 127 cases of different gastric pathological changes were respectively determined by in situ hybridization,Warthin-Starry and immunohistochemistry staining. Results The positive staining rates of TERT mRNA and PCNA in GC were respectively higher than those in gastric precancerous lesion group (P＜0.05). In Hp positive group, the expression of TERT mRNA and PCNA were higher than that in Hp negative group(P＜0.01). Conclusions Helicobacter pylori can stimulate gastric mucosa to excessive proliferate,at the same time,can induce the telomerase to activate and the disorder expression of PCNA, thereby, priming and accelerating the pathogenesis and development of gastric carcinoma.The excessive expression of PCNA and TERT are related to gastric carcinoma.Using them to screening high risk of precancerous lesion of gastric carcinoma.
