肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2009年
1期
11-13
,共3页
杨芳%张聪%白喜花%赵娜%闫磊%杨永明%任连生
楊芳%張聰%白喜花%趙娜%閆磊%楊永明%任連生
양방%장총%백희화%조나%염뢰%양영명%임련생
苏木精%细胞系,肿瘤%肝肿瘤,实验性%免疫
囌木精%細胞繫,腫瘤%肝腫瘤,實驗性%免疫
소목정%세포계,종류%간종류,실험성%면역
Haematoxyhn%Cell line,tumor,Liver neoplasms,exprimental%Immunization
目的 观察苏木精修饰的肿瘤细胞瘤苗对小鼠移植性肝癌HepA的抑瘤作用.方法 小鼠HepA细胞经0.1%的苏木精处理后,制备成三种瘤苗:弗氏完全佐剂瘤苗、弗氏不完全佐剂瘤苗、不含佐剂的肿瘤细胞瘤苗.分别用三种瘤苗免疫小鼠5次后,以活的HepA细胞1×105/鼠腹腔接种攻击,观察各组动物的平均生存时间,比较三种瘤苗对荷瘤动物的免疫保护作用,以等量生理盐水代替瘤苗作对照组.结果 对照组小鼠平均生存时间为(23.30±1.24)d;弗氏完全佐剂组为(43.90±15.20)d(P<0.02);弗氏不完全佐剂组为(39.60±13.77)d(P<0.05);不含佐剂组为(38.40±12.54)d(P<0.05).与对照组比较,各组动物的生命延长率分别为88.41%、69.96%、64.81%.结论 经苏木精修饰的小鼠肝癌瘤苗对荷瘤小鼠可产生显著的保护作用;弗氏佐剂对肿瘤细胞瘤苗免疫效果有增强的作用.
目的 觀察囌木精脩飾的腫瘤細胞瘤苗對小鼠移植性肝癌HepA的抑瘤作用.方法 小鼠HepA細胞經0.1%的囌木精處理後,製備成三種瘤苗:弗氏完全佐劑瘤苗、弗氏不完全佐劑瘤苗、不含佐劑的腫瘤細胞瘤苗.分彆用三種瘤苗免疫小鼠5次後,以活的HepA細胞1×105/鼠腹腔接種攻擊,觀察各組動物的平均生存時間,比較三種瘤苗對荷瘤動物的免疫保護作用,以等量生理鹽水代替瘤苗作對照組.結果 對照組小鼠平均生存時間為(23.30±1.24)d;弗氏完全佐劑組為(43.90±15.20)d(P<0.02);弗氏不完全佐劑組為(39.60±13.77)d(P<0.05);不含佐劑組為(38.40±12.54)d(P<0.05).與對照組比較,各組動物的生命延長率分彆為88.41%、69.96%、64.81%.結論 經囌木精脩飾的小鼠肝癌瘤苗對荷瘤小鼠可產生顯著的保護作用;弗氏佐劑對腫瘤細胞瘤苗免疫效果有增彊的作用.
목적 관찰소목정수식적종류세포류묘대소서이식성간암HepA적억류작용.방법 소서HepA세포경0.1%적소목정처리후,제비성삼충류묘:불씨완전좌제류묘、불씨불완전좌제류묘、불함좌제적종류세포류묘.분별용삼충류묘면역소서5차후,이활적HepA세포1×105/서복강접충공격,관찰각조동물적평균생존시간,비교삼충류묘대하류동물적면역보호작용,이등량생리염수대체류묘작대조조.결과 대조조소서평균생존시간위(23.30±1.24)d;불씨완전좌제조위(43.90±15.20)d(P<0.02);불씨불완전좌제조위(39.60±13.77)d(P<0.05);불함좌제조위(38.40±12.54)d(P<0.05).여대조조비교,각조동물적생명연장솔분별위88.41%、69.96%、64.81%.결론 경소목정수식적소서간암류묘대하류소서가산생현저적보호작용;불씨좌제대종류세포류묘면역효과유증강적작용.
Objective To observe the immunological effect of tumor cell vaccines to mouse hepatoma A(HepA) treated by Haematoxylin. Methods HepA cell was treated by 0.1% Haematoxylin and made into three vaccines: HepA vaccine with complete Freund' s adjuvant, HepA vaccine with incomplete Freund' s adjuvant; and HepA vaccine without any adjuvants. Five times of immunization were given the grouped mice with the above three vaccines, then active HepA cells (1×105 for each mouse) were inoculated by intraperitoneal injection to attack them; reckoning the mean survival time (MST) of the grouped mice, comparing the immunoprotective action of the three vaccines to the tmnor-bearing mice. Those mice only receiving normal saline (equal volume to the vaccine) were as a control group. Results MST of control group was (23.30±1.24) day; MST of mice receiving H22 vaccine with complete adjuvant was (43.90±15.20) day (P<0.02); MST of mice receiving H22 vaccine with incomplete adjuvant was (39.60±13.77) day (P<0.05); and MST of mice receiving HepA vaccine without any adjuvant was (38.40±12.54) day (P<0.05); As compared with the control group, the three treated groups showed a life-lengthening rate (LLR) 88.41%, 69.96 % and 64.81% respectively. Conclusion The vaccines treated by Haematoxylin give a marked immunoprotective action to those tumor-bearing mice. The HepA vaccine' s immunological effect is increased by the Freund' s adjuvant (complete or incomplete).
