CAJ | 학술논문

目的通过检测DNA修复酶PARP在肝细胞性肝癌(hepatocellular carcinoma, HCC)及肝硬化组织中的表达,探讨PARP蛋白在HCC和肝硬化组织中的表达特点及其与临床病理因素的关系.方法应用EnVision免疫组化法检测41例非HCC正常肝组织、99例HCC、51例HCC癌旁肝硬化组织中PARP蛋白表达情况.结果在HCC组织中PARP蛋白在细胞核与细胞质中均可表达,其中PARP细胞核阳性分度在正常组、肝硬化组、HCC组之间依次降低(P＜0.001).多因素分析显示PARP蛋白在细胞质表达与外周血白细胞数(OR=0.757,P=0.037)有关.结论在HCC发生过程中,PARP蛋白在肝细胞核中的表达可能随着病程进展而降低,反映了从肝硬化到肝癌进程中肝细胞内氧化应激状态的改变.
목적 통과검측DNA수복매PARP재간세포성간암(hepatocellular carcinoma, HCC)급간경화조직중적표체,탐토PARP단백재HCC화간경화조직중적표체특점급기여림상병리인소적관계.방법 응용EnVision면역조화법검측41례비HCC정상간조직、99례HCC、51례HCC암방간경화조직중PARP단백표체정황.결과 재HCC조직중PARP단백재세포핵여세포질중균가표체,기중PARP세포핵양성분도재정상조、간경화조、HCC조지간의차강저(P＜0.001).다인소분석현시PARP단백재세포질표체여외주혈백세포수(OR=0.757,P=0.037)유관.결론 재HCC발생과정중,PARP단백재간세포핵중적표체가능수착병정진전이강저,반영료종간경화도간암진정중간세포내양화응격상태적개변.
Objective To explore the expression and the clinicopathological implications of poly ADP-ribose polymerase (PARP) in hepatocellular carcinoma (HCC) tissues and cirrhotic tissues.Methods PARP expression in the tissues of HCC (n=99), cirrhosis (n=51) and normal control (n=41) were detected by immunohistochemical EnVision method.Results There were three types of PARP positive staining in HCC tissues: nuclear, cytoplasmic and mixed.The PARP expression level of nuclear type was significantly lower in HCC tissues than those in cirrhosis and normal control, and was significantly lower in cirrhosis than that in normal control (P＜0.01).The expression of PARP was not associated with the clinicopathological factors in HCC cases (P＞0.05).Multiplicity analysis indicated that PARP expression in cytoplasm was related to the number of peripheral blood leucocytes (OR= 0.757,P=0.037).Conclusion sPARP expression in liver cell nucleus might reflect the status of oxidative stress in each stage during hepatocarcinogenesis, which might implicate the etiology of HCC.