CAJ | 학술논문

测定了乌桕梓油常规理化特性.采用柱层析、薄层层析和气相色谱法,研究了乌桕梓油中甘油酯种类及其脂肪酸组成.采用高温气相色谱/EI质谱联用仪直接分析了乌桕梓油中甘油酯结构.结果表明,乌桕梓油中性脂占98.79%,磷脂占0.22%,糖脂占0.99%.乌桕梓油中性脂的脂肪酸组成主要有C12:0、C14:0、C16:0、C17:0、C18:0、C18:1、C18:2和C18:3,磷脂中没有检出C12:0和C17:0,糖脂中则没有检出C17:0.乌桕梓油中甘油酯组成主要有月桂酸棕榈酸亚油酸甘油三酯、硬脂酸亚油酸亚麻甘油三酯、双棕榈酸亚油酸甘油三酯、棕榈酸亚油酸亚麻酸甘油三酯和双亚油酸亚麻酸甘油三酯等5种.最后,比较了无溶剂体系和叔丁醇体系中,以杂醇油为酰基受体,初步研究了脂肪酶法制备乌桕梓油生物柴油效果.结果表明,对于无溶剂体系和叔丁醇体系而言,当Novozym435与Lipozyme TLIM脂肪酶复合比例为2:4时,生物柴油转化率达到最高,分别为98.28%和76.33%.
측정료오구재유상규이화특성.채용주층석、박층층석화기상색보법,연구료오구재유중감유지충류급기지방산조성.채용고온기상색보/EI질보련용의직접분석료오구재유중감유지결구.결과표명,오구재유중성지점98.79%,린지점0.22%,당지점0.99%.오구재유중성지적지방산조성주요유C12:0、C14:0、C16:0、C17:0、C18:0、C18:1、C18:2화C18:3,린지중몰유검출C12:0화C17:0,당지중칙몰유검출C17:0.오구재유중감유지조성주요유월계산종려산아유산감유삼지、경지산아유산아마감유삼지、쌍종려산아유산감유삼지、종려산아유산아마산감유삼지화쌍아유산아마산감유삼지등5충.최후,비교료무용제체계화숙정순체계중,이잡순유위선기수체,초보연구료지방매법제비오구재유생물시유효과.결과표명,대우무용제체계화숙정순체계이언,당Novozym435여Lipozyme TLIM지방매복합비례위2:4시,생물시유전화솔체도최고,분별위98.28%화76.33%.
The physico - chemical properties of Chinese tallow kernel oil were determined, and the fatty acid compo-sition of the oil was studied with column chromatography, thin - layer chromatography and gas chromatography. Results show:Neutral fat, phospholipid and glycolipid accounts for98.79%, 0.22% and 0.99%, respectively in the Chinese tal-low kernel oil. The fatty acid components of the neutral fat are C12:0, C14:0, C16:0, C17:0, C18:0, C18: 1, C18:2 and C18:3, while the fatty acid composition of the phospholiid is free of C12:0 and C17:0, and the fatty acid composition of the glycolipid is free of C17:0. Five types of triglyeeride are included in Chinese tallow kernel oil, and the prominent type is LLLn triglyceride. Finally, production of biodiesel from Chinese tallow kernel oil using lipase as biocatalyst was ex-plored in solvent free system and t - butanol system, respectively. With Novozym 435 and Lipozyme TLIM complex ratio of 2:4, the biodiesel translation rate for solvent free system and t- butanol system is up to 98.28 % and 76.33 %, respec-tively.