中国药理学通报
中國藥理學通報
중국약이학통보
CHINESE PHARMACOLOGICAL BULLETIN
2010年
2期
174-177
,共4页
成人%肝癌%褪黑素%褪黑素受体%褪黑素受体1%褪黑素受体2
成人%肝癌%褪黑素%褪黑素受體%褪黑素受體1%褪黑素受體2
성인%간암%퇴흑소%퇴흑소수체%퇴흑소수체1%퇴흑소수체2
adult%hepatoma%melatonin%melatonin receptor%melatonin receptor1%melatonin receptor2
目的 检测成人肝癌组织是否表达褪黑素受体(melatonin receptor,MR),并探讨其结合特性.方法 免疫组化法检测肝癌组织MR两种亚型(MT1、MT2)的表达和分布.采用放射配体结合法检测肝癌组织MR特异性结合和动力学特性.结果 免疫组化结果 显示,成人肝癌组织MT1和MT2均呈聚集型粽黄色颗粒阳性染色,主要分布在细胞膜和细胞质,细胞核中表达最少.放射配体结合结果 表明,成人肝癌组织存有~(125)I标记的褪黑素(~(125)I-Mel)特异结合位点,最大结合容量(B_(max))值为(0.29±0.07) pmol·g~(-1) protein,平衡解离常数(K_d)值为(48.7±6.5) pmol·L~(-1),动力学分析表明该特异性结合特点为可饱和及可逆性结合.结论 成人肝癌组织表达MR,包括MT1和MT2,其结合特性为低结合容量、高亲和力、可饱和性及可逆性.
目的 檢測成人肝癌組織是否錶達褪黑素受體(melatonin receptor,MR),併探討其結閤特性.方法 免疫組化法檢測肝癌組織MR兩種亞型(MT1、MT2)的錶達和分佈.採用放射配體結閤法檢測肝癌組織MR特異性結閤和動力學特性.結果 免疫組化結果 顯示,成人肝癌組織MT1和MT2均呈聚集型粽黃色顆粒暘性染色,主要分佈在細胞膜和細胞質,細胞覈中錶達最少.放射配體結閤結果 錶明,成人肝癌組織存有~(125)I標記的褪黑素(~(125)I-Mel)特異結閤位點,最大結閤容量(B_(max))值為(0.29±0.07) pmol·g~(-1) protein,平衡解離常數(K_d)值為(48.7±6.5) pmol·L~(-1),動力學分析錶明該特異性結閤特點為可飽和及可逆性結閤.結論 成人肝癌組織錶達MR,包括MT1和MT2,其結閤特性為低結閤容量、高親和力、可飽和性及可逆性.
목적 검측성인간암조직시부표체퇴흑소수체(melatonin receptor,MR),병탐토기결합특성.방법 면역조화법검측간암조직MR량충아형(MT1、MT2)적표체화분포.채용방사배체결합법검측간암조직MR특이성결합화동역학특성.결과 면역조화결과 현시,성인간암조직MT1화MT2균정취집형종황색과립양성염색,주요분포재세포막화세포질,세포핵중표체최소.방사배체결합결과 표명,성인간암조직존유~(125)I표기적퇴흑소(~(125)I-Mel)특이결합위점,최대결합용량(B_(max))치위(0.29±0.07) pmol·g~(-1) protein,평형해리상수(K_d)치위(48.7±6.5) pmol·L~(-1),동역학분석표명해특이성결합특점위가포화급가역성결합.결론 성인간암조직표체MR,포괄MT1화MT2,기결합특성위저결합용량、고친화력、가포화성급가역성.
Aim To determine whether there existed melatonin receptor (MR) in adult hepatoma tissue and to observe its property of binding.Methods Immuno-histochemistry was used to detect MR and identify its subcellular distribution. Specific binding and kinetic analyses of melatonin receptor were measured by radioligand binding assay.Results The results of immunohistochemistry showed that MT1 and MT2 existed in membrane, cytosol and nucleus of the hepatoma cells, but the expression of them principally localized in the membrane and cytosol. The specific binding assay properties of MR were presented as follows: the maximum binding capacity (B_(max)) was (0.29±0.07) pmol·g~(-1) protein. Equilibrium dissociation constant (K_d) was (48.7±6.5) pmol·L~(-1). The trait of MR, saturation and reversibility, was detected by ~(125)I-Mel specific binding kinetic analyses.Conclusions MR exists in adult hepatoma cells, furthermore, the subtypes of MR (MT1 and MT2) coexist in the membrane and cytosol, respectively,whose characters of the specific binding sites were low binding capacity,high affinity,saturation and reversibility.
