北京中医药大学学报
北京中醫藥大學學報
북경중의약대학학보
JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
2009年
10期
702-705
,共4页
杜英峰%靳怡然%张兰桐%刘静荣%张立段
杜英峰%靳怡然%張蘭桐%劉靜榮%張立段
두영봉%근이연%장란동%류정영%장립단
高效液相色谱%抗感解毒口服液%黄芩苷%绿原酸%栀子苷
高效液相色譜%抗感解毒口服液%黃芩苷%綠原痠%梔子苷
고효액상색보%항감해독구복액%황금감%록원산%치자감
HPLC%Kangganjiedu Oral Solution%baicalin%chlorogenic acid%jasminoidin
目的 建立同时测定抗感解毒口服液中黄芩苷、绿原酸和栀子苷的分析方法.方法 采用多波长RP-HPLC法,色谱柱为Agilent Zorbax SB C18柱(250 mm×4.6 mm, 5 μm),流动相:0.3%的甲酸水溶液(A)、乙腈(B);线性梯度洗脱,B:14%～20%,0～12 min;B:20%～30%,12～13min;B:30%～65%,13～25 min;B:65%,25～35 min;柱温:30 ℃;流速:1.0 mL/min.结果 黄芩苷、绿原酸和栀子苷分别在0.225～3.60 μg,0.023 0～0.368 0 μg,0.135～2.16μg的范围内线形关系良好,平均回收率分别为99.62%、99.64%、100.3%,RSD分别为0.68%、1.5%、0.23%.结论 本法简便、准确、重现性好,可用于抗感解毒口服液中黄芩苷、绿原酸和栀子苷的同时测定.
目的 建立同時測定抗感解毒口服液中黃芩苷、綠原痠和梔子苷的分析方法.方法 採用多波長RP-HPLC法,色譜柱為Agilent Zorbax SB C18柱(250 mm×4.6 mm, 5 μm),流動相:0.3%的甲痠水溶液(A)、乙腈(B);線性梯度洗脫,B:14%～20%,0～12 min;B:20%～30%,12～13min;B:30%～65%,13～25 min;B:65%,25～35 min;柱溫:30 ℃;流速:1.0 mL/min.結果 黃芩苷、綠原痠和梔子苷分彆在0.225～3.60 μg,0.023 0～0.368 0 μg,0.135～2.16μg的範圍內線形關繫良好,平均迴收率分彆為99.62%、99.64%、100.3%,RSD分彆為0.68%、1.5%、0.23%.結論 本法簡便、準確、重現性好,可用于抗感解毒口服液中黃芩苷、綠原痠和梔子苷的同時測定.
목적 건립동시측정항감해독구복액중황금감、록원산화치자감적분석방법.방법 채용다파장RP-HPLC법,색보주위Agilent Zorbax SB C18주(250 mm×4.6 mm, 5 μm),류동상:0.3%적갑산수용액(A)、을정(B);선성제도세탈,B:14%～20%,0～12 min;B:20%～30%,12～13min;B:30%～65%,13～25 min;B:65%,25～35 min;주온:30 ℃;류속:1.0 mL/min.결과 황금감、록원산화치자감분별재0.225～3.60 μg,0.023 0～0.368 0 μg,0.135～2.16μg적범위내선형관계량호,평균회수솔분별위99.62%、99.64%、100.3%,RSD분별위0.68%、1.5%、0.23%.결론 본법간편、준학、중현성호,가용우항감해독구복액중황금감、록원산화치자감적동시측정.
Objective To set up a method for determining and analyzing baicalin, chlorogenic acid and jasminoidin in Kangganjiedu Oral Solution simultaneously by multi-wavelength RP-HPLC. Methods The procedure of multi-wavelength RP-HPLC was performed on the chromatographic column of Agilent Zorbax SB C_(18)(250 mm ×4.6 mm, 5 μm). The mobile phase of linear gradient elution was 0. 3% aqueous formic acid solution (A) to acetonitrile (B) , and performed as follows; B: 14% -20% ,0 -12 min; B: 20% -30% ,12-13 min; B: 30% -65% ,13 -25 min; B: 65% ,25 -35 min. The flow rate was 1.0 mL/min. The column temperature was 30 ℃. Results Baicalin showed a good linear relationship at a range of 0. 225 - 3. 60 μg, chlorogenic acid, at 0. 023 0-0. 368 0 μg, and jasminoidin, at 0. 135 -2. 16 μg. The average recovery of baicalin, chlorogenic acid and jasminoidin was 99.62% , 99.64% and 100. 3% respectively. RSD was 0. 68% , 1.5% and 0. 23% respectively. Conclusion The method was easy and accurate with a higher repeatability, which can be used in the determination of baicalin, chlorogenic acid and jasminoidin in Kangganjiedu Oral Solution simultaneously.
