白血病·淋巴瘤
白血病·淋巴瘤
백혈병·림파류
JOURNAL OF LEUKEMIA & LYMPHOMA
2012年
5期
282-284
,共3页
张苗%张秀莲%张伟华%范星火%葛晓静%魏芳%陶洁
張苗%張秀蓮%張偉華%範星火%葛曉靜%魏芳%陶潔
장묘%장수련%장위화%범성화%갈효정%위방%도길
多发性骨髓瘤骨病%白细胞介素-17%核因子κB受体活化因子配体
多髮性骨髓瘤骨病%白細胞介素-17%覈因子κB受體活化因子配體
다발성골수류골병%백세포개소-17%핵인자κB수체활화인자배체
Multiple myeloma bone disease%Interleukin-17%Receptor activator of nuclear factor-kappaB
目的 研究多发性骨髓瘤(MM)骨病患者骨髓中白细胞介素17(IL-17)的表达水平及其与骨髓单个核细胞核因子κB受体活化因子配体(RANKL)表达的关系,探讨IL-17在MM骨病发病机制中的作用及其临床意义.方法 采用双抗体夹心酶联免疫吸附(ELISA)法检测33例MM骨病患者及20例对照者骨髓上清中IL-17水平,采用荧光定量PCR检测上述两组骨髓单个核细胞RANKL mRNA的表达.结果 MM骨病组及对照组的骨髓上清均表达IL-17,骨髓单个核细胞均表达RANKL mRNA.MM骨病组骨髓上清中IL-17的含量[(52.69±4.55) pg/ml]高于对照组[(14.35±1.25) pg /ml],MM骨病组骨髓单个核细胞RANKL mRNA的表达[(0.96±0.12 )pg/ml]高于对照组[(0.42±0.03) pg/ml],差异均有统计学意义(P＜0.05).活动期MM骨病患者骨髓IL-17[(76.71±7.06)pg/ml]水平显著高于稳定期MM骨病患者[(40.67±3.84)pg/ml],差异有统计学意义(P＜0.05),活动期MM骨病患者骨髓单个核细胞RANKL mRNA的表达水平(1.22±0.27)显著高于稳定期MM骨病患者(0.83±0.12),差异有统计学意义(P＜0.05).MM骨病组骨髓IL-17与RANKL的表达呈显著正相关(r=0.690,P＜0.05).结论 MM骨病患者骨髓IL-17的表达显著增高,骨髓IL-17水平与MM活动期和(或)稳定期相关,骨髓IL-17与RANKL的表达呈正相关,IL-17可能在MM骨病的发病机制中起重要的作用.
目的 研究多髮性骨髓瘤(MM)骨病患者骨髓中白細胞介素17(IL-17)的錶達水平及其與骨髓單箇覈細胞覈因子κB受體活化因子配體(RANKL)錶達的關繫,探討IL-17在MM骨病髮病機製中的作用及其臨床意義.方法 採用雙抗體夾心酶聯免疫吸附(ELISA)法檢測33例MM骨病患者及20例對照者骨髓上清中IL-17水平,採用熒光定量PCR檢測上述兩組骨髓單箇覈細胞RANKL mRNA的錶達.結果 MM骨病組及對照組的骨髓上清均錶達IL-17,骨髓單箇覈細胞均錶達RANKL mRNA.MM骨病組骨髓上清中IL-17的含量[(52.69±4.55) pg/ml]高于對照組[(14.35±1.25) pg /ml],MM骨病組骨髓單箇覈細胞RANKL mRNA的錶達[(0.96±0.12 )pg/ml]高于對照組[(0.42±0.03) pg/ml],差異均有統計學意義(P＜0.05).活動期MM骨病患者骨髓IL-17[(76.71±7.06)pg/ml]水平顯著高于穩定期MM骨病患者[(40.67±3.84)pg/ml],差異有統計學意義(P＜0.05),活動期MM骨病患者骨髓單箇覈細胞RANKL mRNA的錶達水平(1.22±0.27)顯著高于穩定期MM骨病患者(0.83±0.12),差異有統計學意義(P＜0.05).MM骨病組骨髓IL-17與RANKL的錶達呈顯著正相關(r=0.690,P＜0.05).結論 MM骨病患者骨髓IL-17的錶達顯著增高,骨髓IL-17水平與MM活動期和(或)穩定期相關,骨髓IL-17與RANKL的錶達呈正相關,IL-17可能在MM骨病的髮病機製中起重要的作用.
목적 연구다발성골수류(MM)골병환자골수중백세포개소17(IL-17)적표체수평급기여골수단개핵세포핵인자κB수체활화인자배체(RANKL)표체적관계,탐토IL-17재MM골병발병궤제중적작용급기림상의의.방법 채용쌍항체협심매련면역흡부(ELISA)법검측33례MM골병환자급20례대조자골수상청중IL-17수평,채용형광정량PCR검측상술량조골수단개핵세포RANKL mRNA적표체.결과 MM골병조급대조조적골수상청균표체IL-17,골수단개핵세포균표체RANKL mRNA.MM골병조골수상청중IL-17적함량[(52.69±4.55) pg/ml]고우대조조[(14.35±1.25) pg /ml],MM골병조골수단개핵세포RANKL mRNA적표체[(0.96±0.12 )pg/ml]고우대조조[(0.42±0.03) pg/ml],차이균유통계학의의(P＜0.05).활동기MM골병환자골수IL-17[(76.71±7.06)pg/ml]수평현저고우은정기MM골병환자[(40.67±3.84)pg/ml],차이유통계학의의(P＜0.05),활동기MM골병환자골수단개핵세포RANKL mRNA적표체수평(1.22±0.27)현저고우은정기MM골병환자(0.83±0.12),차이유통계학의의(P＜0.05).MM골병조골수IL-17여RANKL적표체정현저정상관(r=0.690,P＜0.05).결론 MM골병환자골수IL-17적표체현저증고,골수IL-17수평여MM활동기화(혹)은정기상관,골수IL-17여RANKL적표체정정상관,IL-17가능재MM골병적발병궤제중기중요적작용.
Objective To detect IL-17 level of bone marrow in patients with multiple myeloma bone disease,and to investigate its clinical significance.Methods The bone marrow IL-17 levels were quantified in 33 cases of multiple myeloma patients and 20 normal control by ELISA assay. RANKL mRNA expression were detected by using RT-PCR.Results In bone marrow supernatant,IL-17 was detected in both groups,and RANKL mRNA were detected in both groups too. IL-17 and RANKL mRNA levels in bone marrow of patients with MM were significantly higher than those in the control group (P＜0.05). The bone marrow concentrations of IL-17 and bone marrow mononuclear cells' RANKL mRNA expression in active stage were significantly higher than those in stable stage (P＜0.05).The bone marrow IL-17 and RANKL were significantly correlated (r =690,P＜0.05).Conclusion IL-17 may play an important role in the pathogenesis of MM.
