CAJ | 학술논문

用热水从香菇子实体中浸提出香菇多糖,采用两种超滤陶瓷膜将粗多糖分级成三部分Le1,Le2和Le3.所有的这三种多糖都由两组分所组成,采用凝胶过滤色谱测定了多糖分子量,13C NMR 和 IR光谱测定显示多糖Le1为含α糖甙键的多糖,多糖Le3为含β糖甙键的多糖.采用气相色谱法测定了三种多糖的单糖组成,结果显示三种多糖都由葡糖糖,阿拉伯糖,木糖,甘露糖和半乳糖组成,Le1, Le2和Le3中阿拉伯糖、木糖、甘露糖、半乳糖、葡萄糖的摩尔比分别为0.15:0.52:1.00:1.20:7.20、0.21:0.68:1.00:1.02:11.56、0.29:0.42:1.00:0.85:16.20.三种多糖Le1, Le2和Le3的平均分子量分别为4.02×104、2.16×105 和 8.93×105.
용열수종향고자실체중침제출향고다당,채용량충초려도자막장조다당분급성삼부분Le1,Le2화Le3.소유적저삼충다당도유량조분소조성,채용응효과려색보측정료다당분자량,13C NMR 화 IR광보측정현시다당Le1위함α당대건적다당,다당Le3위함β당대건적다당.채용기상색보법측정료삼충다당적단당조성,결과현시삼충다당도유포당당,아랍백당,목당,감로당화반유당조성,Le1, Le2화Le3중아랍백당、목당、감로당、반유당、포도당적마이비분별위0.15:0.52:1.00:1.20:7.20、0.21:0.68:1.00:1.02:11.56、0.29:0.42:1.00:0.85:16.20.삼충다당Le1, Le2화Le3적평균분자량분별위4.02×104、2.16×105 화 8.93×105.
The Lentinan was extracted by hot water from the fruits bodies of Lentinus edodes,and separated by two kinds of ultrafiltration membranes to yield three water soluble polysaccharides Le1,Le2 and Le3. They are consisted of two components. Their molecular weight was determined using gel-filtration chromatography. The 13C NMR and IR spectra confirmed that the sugar residue of Le1 was α-glycosidically linked, the sugar residue of Le3 was β-glycosidically linked. The polysaccharides component were determined by gas chromatography, three lentinans were composed of glucose (Glu), arabinose (Ara), xylose (Xyl), mannose (Man) and galactose (Gla). The neutral saccharide molar ratios Ara: Xyl: Man: Gal: Glu are as follows: Le1,0.15∶0.52∶1.00∶1.20∶7.20; Le2, 0.21∶0.68∶1.00∶1.02∶11.56; Le3, 0.29∶0.42∶1.00∶0.85∶16.20. The average molecular weight (Mw) of the three polysaccharides Le1, Le2 and Le3 were determined to be 4.02×104, 2.16×105, 8.93×105, respectively.