安徽农业科学
安徽農業科學
안휘농업과학
JOURNAL OF ANHUI AGRICULTURAL SCIENCES
2009年
36期
17862-17864
,共3页
朱露娟%胡娜%周斌%常军
硃露娟%鬍娜%週斌%常軍
주로연%호나%주빈%상군
HPLC%哈巴苷%哈巴苷代谢素Ⅰ%哈巴苷代谢素Ⅱ%桃叶珊瑚甙B
HPLC%哈巴苷%哈巴苷代謝素Ⅰ%哈巴苷代謝素Ⅱ%桃葉珊瑚甙B
HPLC%합파감%합파감대사소Ⅰ%합파감대사소Ⅱ%도협산호대B
HPLC%Harpagide%HarpagidemetabolineⅠ%Harpagidemetaboline Ⅱ%Aucubin B
[目的]研究黑曲霉转化液中哈巴苷及其3个转化产物的同时检测方法.[方法]通过用HPLC-SPE法快速测定黑曲霉转化液中哈巴苷及其3个转化产物的含量考察了该法的应用条件.[结果]哈巴苷、哈巴苷代谢素Ⅰ、哈巴苷代谢素Ⅱ和桃叶珊瑚甙B的线性回归方程分别为y= 52.32x-89.76、y=32.52x-45.26、y=14.73x-25.64和y=41.25x-33.71,最低检测浓度分别为0.020、0.010、0.015和0.015 μg/ml.0.010、0.100和2.500 μg/ml哈巴苷的回收率分别为103.0%、99.7%和99.9%,0.010、0.500和2.500 μg/ml的哈巴苷代谢素Ⅰ的回收率分别为102.1%、99.8%和99.9%,0.015、0.500和2.500 μg/ml的哈巴苷代谢素Ⅱ的回收率分别为99.3%、99.6%和99.6%,0.015、0.500和2.500 μg/ml的桃叶珊瑚甙B的回收率分别为100.6%、100.7%和99.9%.该法检测哈巴苷、哈巴苷代谢素Ⅰ、哈巴苷代谢素Ⅱ和桃叶珊瑚甙B的相对标准偏差在1.3%～4.7%.[结论]该方法能够快速、高效地检测黑曲霉转化液中哈巴苷及其转化产物的含量.
[目的]研究黑麯黴轉化液中哈巴苷及其3箇轉化產物的同時檢測方法.[方法]通過用HPLC-SPE法快速測定黑麯黴轉化液中哈巴苷及其3箇轉化產物的含量攷察瞭該法的應用條件.[結果]哈巴苷、哈巴苷代謝素Ⅰ、哈巴苷代謝素Ⅱ和桃葉珊瑚甙B的線性迴歸方程分彆為y= 52.32x-89.76、y=32.52x-45.26、y=14.73x-25.64和y=41.25x-33.71,最低檢測濃度分彆為0.020、0.010、0.015和0.015 μg/ml.0.010、0.100和2.500 μg/ml哈巴苷的迴收率分彆為103.0%、99.7%和99.9%,0.010、0.500和2.500 μg/ml的哈巴苷代謝素Ⅰ的迴收率分彆為102.1%、99.8%和99.9%,0.015、0.500和2.500 μg/ml的哈巴苷代謝素Ⅱ的迴收率分彆為99.3%、99.6%和99.6%,0.015、0.500和2.500 μg/ml的桃葉珊瑚甙B的迴收率分彆為100.6%、100.7%和99.9%.該法檢測哈巴苷、哈巴苷代謝素Ⅰ、哈巴苷代謝素Ⅱ和桃葉珊瑚甙B的相對標準偏差在1.3%～4.7%.[結論]該方法能夠快速、高效地檢測黑麯黴轉化液中哈巴苷及其轉化產物的含量.
[목적]연구흑곡매전화액중합파감급기3개전화산물적동시검측방법.[방법]통과용HPLC-SPE법쾌속측정흑곡매전화액중합파감급기3개전화산물적함량고찰료해법적응용조건.[결과]합파감、합파감대사소Ⅰ、합파감대사소Ⅱ화도협산호대B적선성회귀방정분별위y= 52.32x-89.76、y=32.52x-45.26、y=14.73x-25.64화y=41.25x-33.71,최저검측농도분별위0.020、0.010、0.015화0.015 μg/ml.0.010、0.100화2.500 μg/ml합파감적회수솔분별위103.0%、99.7%화99.9%,0.010、0.500화2.500 μg/ml적합파감대사소Ⅰ적회수솔분별위102.1%、99.8%화99.9%,0.015、0.500화2.500 μg/ml적합파감대사소Ⅱ적회수솔분별위99.3%、99.6%화99.6%,0.015、0.500화2.500 μg/ml적도협산호대B적회수솔분별위100.6%、100.7%화99.9%.해법검측합파감、합파감대사소Ⅰ、합파감대사소Ⅱ화도협산호대B적상대표준편차재1.3%～4.7%.[결론]해방법능구쾌속、고효지검측흑곡매전화액중합파감급기전화산물적함량.
[Objective]The purpose was to study the simultaneous determination method of harpagide and its 3 transformed products in the transformation liquid of Aspergillus nige. [Method]The application conditions of HPLC-SPE method were investigated through determining the contents of harpagide and its 3 transformed products in the transformation liquid of A. niger rapidly by this method. [Result] The linear regression equations of harpagide, harpagidemetabolineⅠ, harpagidemetaboline Ⅱ and aucubin B were y= 52.32x-89.76, y=32.52x-45.26, y=14.73x-25.64 and y=41.25x-33.71 resp. Their minimum detectable concn. were 0.020, 0.010, 0.015 and 0.015 μg/ml resp. The recoveries of 0.010, 0.100 and 2.500 μg/ml harpagide were 103.0%, 99.7% and 99.9% resp., that of 0.010, 0.500 and 2.500 μg/ml harpagidemetabolineⅠ were 102.1%, 99.8% and 99.9% resp., that of 0.015, 0.500 and 2.500 μg/ml harpagidemetaboline Ⅱ were 99.3%, 99.6% and 99.6% resp. and that of 0.015, 0.500 and 2.500 μg/ml aucubin B were 100.6%, 100.7% and 99.9% resp. The RSDs of determination on harpagide, harpagidemetabolineⅠ, harpagidemetaboline Ⅱ and aucubin B by this method were in 1.3%～4.7%. [Conclusion] The contents of harpagide and its transformed products in the transformation liquid of A. niger could be determined rapidly and efficiently by this method.
