CAJ | 학술논문

从高温堆肥土样中筛选到一株高产蛋白酶的菌株,经鉴定为Bacillus subtilis,命名为I15.对最适产酶发酵条件进行了优化,并研究了发酵产蛋白酶的酶学性质.结果表明,玉米淀粉和豆饼粉是该菌发酵产蛋白酶的最适经济碳氮源;该蛋白酶属高温碱性蛋白酶,具有很强的热稳定性和酸碱稳定性.另外,突出的性质是该蛋白酶对表面活性剂和温和型去垢剂具有超强的耐受性,将具有广阔的市场前景.
종고온퇴비토양중사선도일주고산단백매적균주,경감정위Bacillus subtilis,명명위I15.대최괄산매발효조건진행료우화,병연구료발효산단백매적매학성질.결과표명,옥미정분화두병분시해균발효산단백매적최괄경제탄담원;해단백매속고온감성단백매,구유흔강적열은정성화산감은정성.령외,돌출적성질시해단백매대표면활성제화온화형거구제구유초강적내수성,장구유엄활적시장전경.
A strain producing higher activity protease named Bacillus subtilis I15 is isolated and identified from waste-rich soil which stacked high temperature and long time. We optimize the fermentation conditions of this strain and study a part of enzymology properties. The results show that corn starch and soybean cake powder are the optimization of this strain as carbon and nitrogen source for thermophilic alkaline protease pro-duetion. The protease belongs to thermophilic alkaline protease,which shows high pH stability and thermal sta-bility. In addition, it shows excellent stability and compatibility with surfactant and detergent. These properties indicate that the protease has a bright market prospect.