CAJ | 학술논문

目的观察α-硫辛酸对高糖作用下血管内皮细胞护骨素(OPG)mRNA表达的影响.方法脐静脉内皮细胞株分别在5.5mmol/L葡萄糖、30mmol/L葡萄糖及30mmol/L葡萄糖+不同浓度α-硫辛酸(50、100、200μmol/L)条件下培养48h.取内皮细胞培养上清液,以黄嘌呤氧化酶法测定超氧化物歧化酶(SOD)活性,以硫代巴比妥酸为底物检测丙二醛(MDA)含量;RT-PCR法检测内皮细胞OPG mRNA表达水平的变化.结果高糖作用下内皮细胞SOD活性降低(P<0.01),MDA水平升高(P<0.01),OPG mRNA表达水平显著上升(P<0.01);以不同浓度α-硫辛酸干预高糖组,SOD活性升高(P<0.01),MDA水平降低(P<0.01),OPG mRNA表达水平显著下降(P<0.05).结论 α-硫辛酸改善高糖环境下血管内皮细胞的氧化应激状态,下调OPG mRNA的表达水平.
목적 관찰α-류신산대고당작용하혈관내피세포호골소(OPG)mRNA표체적영향.방법 제정맥내피세포주분별재5.5mmol/L포도당、30mmol/L포도당급30mmol/L포도당+불동농도α-류신산(50、100、200μmol/L)조건하배양48h.취내피세포배양상청액,이황표령양화매법측정초양화물기화매(SOD)활성,이류대파비타산위저물검측병이철(MDA)함량;RT-PCR법검측내피세포OPG mRNA표체수평적변화.결과 고당작용하내피세포SOD활성강저(P<0.01),MDA수평승고(P<0.01),OPG mRNA표체수평현저상승(P<0.01);이불동농도α-류신산간예고당조,SOD활성승고(P<0.01),MDA수평강저(P<0.01),OPG mRNA표체수평현저하강(P<0.05).결론 α-류신산개선고당배경하혈관내피세포적양화응격상태,하조OPG mRNA적표체수평.
Objective To investigate the effect of αlipoic acid (LA)on expression levels of OPG in human umbilical vein endothelial cells(HUVECs) under high glucose. Methods HUVECs were incubated for 48h with 5.5mmol/L glucose(NG), 30 mmol/L glucose(HG), 30mmol/L glucose plus 50,100 and 200μmol/L of LA (HG+LA50,HG+LA100,HG+LA200,respectively). The activity of SOD in the supernatant fluid was determined by the method of xanthine oxidase and the content of MDA was measured with the substrate of thiobarbituric acid. Reverse transcriptionpolymerase chain reaction (RT-PCR) examination was used for assay of the expression of OPG mRNA. Results High glucose decreased the activity of SOD（P<0.01）and increased the content of MDA（P<0.01）.The expression level of OPG mRNA was increased markedly under high glucose concentration（P<0.01）. As compared with HG group the groups of HG+LA50 or LA100 or LA200 showed that the activity of SOD（P<0.01）was increased, the content of MDA was decreased（P<0.01）, and the expression levels of OPG mRNA was decreased significantly（all P<0.05）. Conclusions The α-lipoic acid can improve oxidative stress state and reduce the expression levels of OPG mRNA in high glucose induced HUVECs.