CAJ | 학술논문

目的观察转化生长因子β1 (TGF-β1)+869T/C基因多态性与自身免疫性甲状腺疾病疾病严重程度的关系.方法重型桥本甲状腺炎患者158例,轻型桥本患者125例；重型Graves病患者129例,轻型Graves病患者130例；健康对照组144例.人组患者及健康对照均选自包头医学院第一附属医院就诊患者及体检健康人群,取肘静脉血,采用序列特异性引物聚合酶链反应(PCR-sequence specific primers,PCR-SSP)方法检测血中TGF-β1 +869T/C基因多态性.结果在桥本甲状腺炎组、Graves病组,TGF-β1 +869T/C基因型、等位基因频率分布变化不明显,与对照组比较差异无统计学意义(x2值分别为1.488、0.439,0.626、0.005；P均＞0.05).重型桥本甲状腺TT基因型及T等位基因频率[34.81％(55/158)、58.86％(186/316)]显著高于轻症病例组[17.60％(22/125)、43.60％(109/250)],组间比较差异有统计学意义(x2值分别为14.040、13.026,P均＜0.05).重型Graves病CC基因型及C等位基因频率[(21.03％(31/129)、51.16％(132/258)]显著高于轻症病例组[(13.85％(18/130)、40.38％(105/260)],组间比较差异有统计学意义(x2值分别为12.225、6.061,P均＜0.05).TGF-β1 +869T/C基因型与桥本甲状腺炎、Graves病组中重型病例存在相关性,C等位基因会增加Graves病的疾病严重程度[比值比(OR)=1.546,95％可信区间(CI)=0.192 - 2.190],而T等位基因则会使桥本甲状腺炎加重(OR=1.851,95％CI=1.323～2.589).结论 TGF-β1+869T/C基因多态性与重度自身免疫性甲状腺病存在相关性.
목적 관찰전화생장인자β1 (TGF-β1)+869T/C기인다태성여자신면역성갑상선질병질병엄중정도적관계.방법 중형교본갑상선염환자158례,경형교본환자125례；중형Graves병환자129례,경형Graves병환자130례；건강대조조144례.인조환자급건강대조균선자포두의학원제일부속의원취진환자급체검건강인군,취주정맥혈,채용서렬특이성인물취합매련반응(PCR-sequence specific primers,PCR-SSP)방법 검측혈중TGF-β1 +869T/C기인다태성.결과 재교본갑상선염조、Graves병조,TGF-β1 +869T/C기인형、등위기인빈솔분포변화불명현,여대조조비교차이무통계학의의(x2치분별위1.488、0.439,0.626、0.005；P균＞0.05).중형교본갑상선TT기인형급T등위기인빈솔[34.81％(55/158)、58.86％(186/316)]현저고우경증병례조[17.60％(22/125)、43.60％(109/250)],조간비교차이유통계학의의(x2치분별위14.040、13.026,P균＜0.05).중형Graves병CC기인형급C등위기인빈솔[(21.03％(31/129)、51.16％(132/258)]현저고우경증병례조[(13.85％(18/130)、40.38％(105/260)],조간비교차이유통계학의의(x2치분별위12.225、6.061,P균＜0.05).TGF-β1 +869T/C기인형여교본갑상선염、Graves병조중중형병례존재상관성,C등위기인회증가Graves병적질병엄중정도[비치비(OR)=1.546,95％가신구간(CI)=0.192 - 2.190],이T등위기인칙회사교본갑상선염가중(OR=1.851,95％CI=1.323～2.589).결론 TGF-β1+869T/C기인다태성여중도자신면역성갑상선병존재상관성.
Objective To clarify whether the +869T/C polymorphism in the transforming growth factor-β1 (TGF-β1) gene is associated with TGF-β1 expression,and involved in the severity of Graves disease(GD) and Hashimoto's thyroiditis(HT).Methods The TGF-β1+869T/C polymorphism was genotyped by using PCR-sequence specific primers(PCR-SSP) in genomic DNA samples in blood from 158 patients with HT who developed hypothyroidism before they were 45 years old (severe HT) and 125 untreated,euthyroid patients with HT who were older than 45(mild HT).Using the same method,129 euthyroid patients with GD who had been under treatment and were still positive for anti-thyrotropin receptor antibodies (intractable GD) and 130 euthyroid patients with GD in remission and 144 healthy controls were examined.Results It had no difference between GD,HT groups and control group (x2 =1.488,0.439; 0.626,0.005; all P ＞ 0.05 ).The frequency of the TT genotype and the T allele were higher in group with severe HT[34.81％(55/158),58.86％( 186/316)] than in those with mild HT[ 17.60％ (22/125),43.60％ (109/250); x2 =14.040,13.026,all P ＜ 0.05].In contrast,the frequency of the CC genotype was higher in group with intractable GD[ (21.03％(31/129),51.16％(132/258)] than in group with GD in remission[ 13.85％ (18/130),40.38％( 105/260); x2 =12.225,6.061,all P ＜ 0.05 ].TGF-β1 +869 T/C genotype had the correlation with severe groups of HT and GD.C allele would increase in severity of GD(OR =1.546,95％ CI =0.192 - 2.190),and T allele would increase in severity of HT(OR =1.851,95％ CI =1.323 - 2.589).Conclusion The +869T/C polymorphism in the TGF-β1 gene is associated with the severity and intractability of autoimmune thyroid disease.