中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2010年
3期
229-232
,共4页
汪际云%渡邊荘%松倉聡%洲崎春海
汪際雲%渡邊荘%鬆倉聡%洲崎春海
왕제운%도변장%송창총%주기춘해
鼻窦炎%鼻息肉%上皮细胞%双RNA病毒科感染%基质金属蛋白酶类%逆转录聚合酶链反应
鼻竇炎%鼻息肉%上皮細胞%雙RNA病毒科感染%基質金屬蛋白酶類%逆轉錄聚閤酶鏈反應
비두염%비식육%상피세포%쌍RNA병독과감염%기질금속단백매류%역전록취합매련반응
Sinusitis%Nasal polyps%Epithelial%Birnaviridae infections%Matrix metalloproteinases%Reverse tranacriptase polymerase chain reaction
目的 病毒感染经常使慢性鼻-鼻窦炎(chronic rhinosinusitis,CRS)病情加重.病毒感染时,基质金属蛋白酶(matrix metalloproteinases,MMPs)调节机制的破坏是导致病情加重的机制之一.本研究试图更好地了解病毒感染后鼻上皮细胞MMPs的表达.方法 取鼻内镜手术时CRS患者的鼻息肉组织,分离培养鼻上皮细胞,以合成的双链RNA类似物--多聚次黄嘌呤胞嘧啶核苷酸[polyinosinic-polycytidylic acid,poly(I:C)]刺激原代鼻上皮细胞后,检测鼻上皮细胞中MMP-2、MMP-9和组织金属蛋白酶抑制物-1(tissue inhibitor of metalloproteinase-1,TIMP-1)mRNA的相对表达量.结果 poly(I:C)刺激后,鼻上皮细胞中MMP-9 mRNA的相对表达量((x-)±s,以下同)较同一患者未刺激的细胞增高(22.61±5.47)倍,差异有统计学意义(Z=-2.52,P=0.012).poly(I:C)刺激后,鼻上皮细胞中MMP-2和TIMP-1 mRNA的相对表达量没有明显改变.结论 双链RNA刺激离体培养的人鼻上皮细胞后,MMP-9 mRNA表达显著增高,且不受TIMP-1的调节.由此可能导致CRS患者病情加重.
目的 病毒感染經常使慢性鼻-鼻竇炎(chronic rhinosinusitis,CRS)病情加重.病毒感染時,基質金屬蛋白酶(matrix metalloproteinases,MMPs)調節機製的破壞是導緻病情加重的機製之一.本研究試圖更好地瞭解病毒感染後鼻上皮細胞MMPs的錶達.方法 取鼻內鏡手術時CRS患者的鼻息肉組織,分離培養鼻上皮細胞,以閤成的雙鏈RNA類似物--多聚次黃嘌呤胞嘧啶覈苷痠[polyinosinic-polycytidylic acid,poly(I:C)]刺激原代鼻上皮細胞後,檢測鼻上皮細胞中MMP-2、MMP-9和組織金屬蛋白酶抑製物-1(tissue inhibitor of metalloproteinase-1,TIMP-1)mRNA的相對錶達量.結果 poly(I:C)刺激後,鼻上皮細胞中MMP-9 mRNA的相對錶達量((x-)±s,以下同)較同一患者未刺激的細胞增高(22.61±5.47)倍,差異有統計學意義(Z=-2.52,P=0.012).poly(I:C)刺激後,鼻上皮細胞中MMP-2和TIMP-1 mRNA的相對錶達量沒有明顯改變.結論 雙鏈RNA刺激離體培養的人鼻上皮細胞後,MMP-9 mRNA錶達顯著增高,且不受TIMP-1的調節.由此可能導緻CRS患者病情加重.
목적 병독감염경상사만성비-비두염(chronic rhinosinusitis,CRS)병정가중.병독감염시,기질금속단백매(matrix metalloproteinases,MMPs)조절궤제적파배시도치병정가중적궤제지일.본연구시도경호지료해병독감염후비상피세포MMPs적표체.방법 취비내경수술시CRS환자적비식육조직,분리배양비상피세포,이합성적쌍련RNA유사물--다취차황표령포밀정핵감산[polyinosinic-polycytidylic acid,poly(I:C)]자격원대비상피세포후,검측비상피세포중MMP-2、MMP-9화조직금속단백매억제물-1(tissue inhibitor of metalloproteinase-1,TIMP-1)mRNA적상대표체량.결과 poly(I:C)자격후,비상피세포중MMP-9 mRNA적상대표체량((x-)±s,이하동)교동일환자미자격적세포증고(22.61±5.47)배,차이유통계학의의(Z=-2.52,P=0.012).poly(I:C)자격후,비상피세포중MMP-2화TIMP-1 mRNA적상대표체량몰유명현개변.결론 쌍련RNA자격리체배양적인비상피세포후,MMP-9 mRNA표체현저증고,차불수TIMP-1적조절.유차가능도치CRS환자병정가중.
Objective Chronic rhinosinusitis was often exacerbated by viral infection.A disruption of the mechanisms that regulate the activity of matrix metallopmteinases(MMPs)during viral infection was one possible mechanism responsible for the cxacerbation.The purpose of study was to achieve a better understanding of MMP expression in hasal epithelial cells after viral infection.Methotis Human nasal epithelial cells were isolated from nasal polyp specimens obtained during endoscopic endonasal surgery in chronic rhinosinusitis patients.The expression of MMP-2,MMP-9,and tissue inhibitor of mctallopmteinase (TIMP).1 mRNA in primary human nasal polyp epithelial cells after double stranded RNA(ds RNA) stimulation were investigated.Results Among the genes whose expression was evaluated,only expression of MMP-9 mRNA increased significantly after dsRNA stimulation(22.61±5.47 fold increase,Z=-2.52,P=0.012).Conclusions The significant up-regulation of MMP-9 mRNA,which Was not modulated by TIMP-1,was an additional siyrec of increased pmteolytic activity in virus-infected upper airways that might contribute to the exacerbation of chronic rhinosinusitis with nasal polyps.
