中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2012年
3期
137-140
,共4页
肝炎e抗原,乙型%肝炎,乙型%肝炎表面抗原,乙型%血清学试验%突变%基因扩增%聚合酶链反应
肝炎e抗原,乙型%肝炎,乙型%肝炎錶麵抗原,乙型%血清學試驗%突變%基因擴增%聚閤酶鏈反應
간염e항원,을형%간염,을형%간염표면항원,을형%혈청학시험%돌변%기인확증%취합매련반응
Hepatitis B e antigens%Hepatitis B%Hepatitis B surface antigens%Serologic tests%Mutation%Gene amplification%Polymerase chain reaction
目的 研究抗-HBe单项阳性患者中隐匿性HBV的感染情况及发生的可能原因.方法 收集HBV血清学标志物检测结果中抗HBe单项强阳性[吸光度(A)值≤0.1]的新鲜血清标本61份,采用实时定量PCR进行HBV DNA含量检测.对于HBV DNA定量阳性的标本,采用雅培试剂复测HBV血清学标志物,采用PCR扩增,并进行克隆测序.结果 61份标本中,2份HBVDNA定量阳性,HBsAg血清学漏检率为3.3％.其中l份标本经雅培试剂复测为抗-HBc单项阳性,其前S区缺失突变和前S2区起始密码子突变,并存在不同突变株混合感染；另1份标本经雅培试剂复测发现,除抗HBe强阳性外,HBsAg和抗- HBc为极弱阳性,未发现前S/S区突变.结论 抗HBe单项阳性患者中存在隐匿性HBV感染和HBsAg血清学漏检情况,后者不仅与前S/S区突变有关,而且与外周血中HBsAg低水平也有一定关系.
目的 研究抗-HBe單項暘性患者中隱匿性HBV的感染情況及髮生的可能原因.方法 收集HBV血清學標誌物檢測結果中抗HBe單項彊暘性[吸光度(A)值≤0.1]的新鮮血清標本61份,採用實時定量PCR進行HBV DNA含量檢測.對于HBV DNA定量暘性的標本,採用雅培試劑複測HBV血清學標誌物,採用PCR擴增,併進行剋隆測序.結果 61份標本中,2份HBVDNA定量暘性,HBsAg血清學漏檢率為3.3％.其中l份標本經雅培試劑複測為抗-HBc單項暘性,其前S區缺失突變和前S2區起始密碼子突變,併存在不同突變株混閤感染；另1份標本經雅培試劑複測髮現,除抗HBe彊暘性外,HBsAg和抗- HBc為極弱暘性,未髮現前S/S區突變.結論 抗HBe單項暘性患者中存在隱匿性HBV感染和HBsAg血清學漏檢情況,後者不僅與前S/S區突變有關,而且與外週血中HBsAg低水平也有一定關繫.
목적 연구항-HBe단항양성환자중은닉성HBV적감염정황급발생적가능원인.방법 수집HBV혈청학표지물검측결과중항HBe단항강양성[흡광도(A)치≤0.1]적신선혈청표본61빈,채용실시정량PCR진행HBV DNA함량검측.대우HBV DNA정량양성적표본,채용아배시제복측HBV혈청학표지물,채용PCR확증,병진행극륭측서.결과 61빈표본중,2빈HBVDNA정량양성,HBsAg혈청학루검솔위3.3％.기중l빈표본경아배시제복측위항-HBc단항양성,기전S구결실돌변화전S2구기시밀마자돌변,병존재불동돌변주혼합감염；령1빈표본경아배시제복측발현,제항HBe강양성외,HBsAg화항- HBc위겁약양성,미발현전S/S구돌변.결론 항HBe단항양성환자중존재은닉성HBV감염화HBsAg혈청학루검정황,후자불부여전S/S구돌변유관,이차여외주혈중HBsAg저수평야유일정관계.
Objective To further explore the occurrence of occult hepatitis B virus (HBV)infection m patients with hepatitis B e antibody (anti-Hbe) positive alone and analyze the possible reasons of occult infection.Methods Sera of 61 patients carrying anti Hbe alone (absorbance≤0.1) were collected and real time polymerase chain reaction (PCR) was used to detect HBV DNA level.HBV markers were detected again by Abbott reagent,preS/S amplification products were obtained by PCR,and clonal sequencings were done in HBV DNA positive samples.Results Two samples were HBV DNA positive in 61 samples with anti-Hbe positive alone,with the hepatitis B surface antigen (HBsAg) miss rate of 3.3％.Sequencing disclosed preS deletion mutations,preS2 initiatior codon mutations and co-existence of the different mutant types in one sample with anti-Hbe positive alone by repeated Abbott detection.No preS/S mutations were found in the other sample with HBsAg and hepatitis B core antibody (anti-HBc) weakly positive by repeated Abbott detection except for anti Hbe strongly positive. Conclusions Occult HBV infection and HBsAg serological test failure exist in patients with anti-Hbe positive alone.The failure of HBsAg detection may be due to preS/S gene mutations as well as low level of circulating HBsAg.
