中国医药
中國醫藥
중국의약
CHINA MEDICINE
2009年
2期
124-125
,共2页
高效液相色谱法%血浆%利多卡因
高效液相色譜法%血漿%利多卡因
고효액상색보법%혈장%리다잡인
High performance liquid chromatography%Blood plasma%Lidocaine
目的 应用高效液相色谱法测定猫血浆中利多卡因浓度,探索试验方法及精确度,以便进一步应用于利多卡因对心血管系统影响的药理学试验.方法 应用高效液相色谱法分别测定对照溶液和猫血浆中利多卡因浓度.结果 猫血浆中内源性物质对测定无干扰;浓度在0.43～427.00 μmol/L范围内与峰面积线性关系良好;峰面积的日内相对标准偏差(RSD)分别为2.16%、1.48%和0.65%;峰面积的日间RSD分别为3.38%、1.78%和0.76%;绝对回收率分别为(84.25±3.54)%、(82.33±3.87)%和(86.38±1.75)%;方法回收率分别为(99.58±5.43)%、(100.88±4.65)%和(99.54±3.65)%.结论 高效液相色谱法稳定、回收率高,并且检测方法简单,适用于大多数的实验室,可应用于药物代谢动力学的研究.
目的 應用高效液相色譜法測定貓血漿中利多卡因濃度,探索試驗方法及精確度,以便進一步應用于利多卡因對心血管繫統影響的藥理學試驗.方法 應用高效液相色譜法分彆測定對照溶液和貓血漿中利多卡因濃度.結果 貓血漿中內源性物質對測定無榦擾;濃度在0.43～427.00 μmol/L範圍內與峰麵積線性關繫良好;峰麵積的日內相對標準偏差(RSD)分彆為2.16%、1.48%和0.65%;峰麵積的日間RSD分彆為3.38%、1.78%和0.76%;絕對迴收率分彆為(84.25±3.54)%、(82.33±3.87)%和(86.38±1.75)%;方法迴收率分彆為(99.58±5.43)%、(100.88±4.65)%和(99.54±3.65)%.結論 高效液相色譜法穩定、迴收率高,併且檢測方法簡單,適用于大多數的實驗室,可應用于藥物代謝動力學的研究.
목적 응용고효액상색보법측정묘혈장중리다잡인농도,탐색시험방법급정학도,이편진일보응용우리다잡인대심혈관계통영향적약이학시험.방법 응용고효액상색보법분별측정대조용액화묘혈장중리다잡인농도.결과 묘혈장중내원성물질대측정무간우;농도재0.43～427.00 μmol/L범위내여봉면적선성관계량호;봉면적적일내상대표준편차(RSD)분별위2.16%、1.48%화0.65%;봉면적적일간RSD분별위3.38%、1.78%화0.76%;절대회수솔분별위(84.25±3.54)%、(82.33±3.87)%화(86.38±1.75)%;방법회수솔분별위(99.58±5.43)%、(100.88±4.65)%화(99.54±3.65)%.결론 고효액상색보법은정、회수솔고,병차검측방법간단,괄용우대다수적실험실,가응용우약물대사동역학적연구.
Objective We attempted to use liquid chromatography to analyze blood plasma concentration of lidocaine in cat, to explore the validation and precision of this method for pharmacological trial of lidocaine in cardiovascular system. Methods Liquid chromatography was used to analyze blood plasma concentration of lidocaine in cat. Results There was endogenous non - interference in cat blood plasma. The equation of linear regression is Y = ax + b, Y = peak area, X = concentration, a = 1. 0234e + 006, b = 4463.42 . The peak area of daily RSD was 16% ,1.48% and 0.65% ; the absolute coefficient of recovery was (84.25±3.54) %, (82.33 ± 3.87) % and (86.38 ± 1.75) % ; the method coefficient of recovery was (99.58 ± 5.43) %, (100.88 ± 4.65) % and (99.54±3.65)% respectively. Conclusion Liquid chromatography is a simple, stable and and efficient test which can be used in lidocaine's pharmacological trial.
