CAJ | 학술논문

取原代培养的SD雄性乳鼠心肌细胞在高糖刺激下加入10-7、10-6和10-5mol/L辛伐他汀作用72 h.结果显示,与对照组比较,高糖组心肌细胞活力明显降低(P＜0.01),乳酸脱氢酶(LDH)活性显著增加(P＜0.01),NADPH氧化酶亚基p22phox、p47phox mRNA表达和活性氧簇(ROS)水平明显增高(均P＜0.05);与高糖组比较,辛伐他汀各组心肌细胞活力明显增加(P＜0.05),LDH活性显著降低(P＜0.05),p22phox、p47phox mRNA表达和ROS水平明显降低,且辛伐他汀浓度对心肌细胞活力的影响呈剂量依赖效应.这些结果提示辛伐他汀能够抑制NADPH氧化酶亚基的基因表达,减轻高糖引起的心肌损伤.
취원대배양적SD웅성유서심기세포재고당자격하가입10-7、10-6화10-5mol/L신벌타정작용72 h.결과현시,여대조조비교,고당조심기세포활력명현강저(P＜0.01),유산탈경매(LDH)활성현저증가(P＜0.01),NADPH양화매아기p22phox、p47phox mRNA표체화활성양족(ROS)수평명현증고(균P＜0.05);여고당조비교,신벌타정각조심기세포활력명현증가(P＜0.05),LDH활성현저강저(P＜0.05),p22phox、p47phox mRNA표체화ROS수평명현강저,차신벌타정농도대심기세포활력적영향정제량의뢰효응.저사결과제시신벌타정능구억제NADPH양화매아기적기인표체,감경고당인기적심기손상.
Ventricular myocytes from hearts of the neonatal SD rats were treated with 10-7,10-6,and 10-5 mol/L simvastatin for 72 hours under high glucose condition. Compared with control group,the viability of cadiomyocyte was significantly lower in high glucose group (P＜0.01 ).The activity of lactate dehydrogenase,the relative expressions of NADPH oxidase subunits p22phox,p47phox mRNA,and reactive oxygen species level in the high glucose group were higher than those of control group ( all P＜0.05).lndexes mentioned above were significantly improved by simvastatin treatment in a dose-dependent manner.These results suggest that simvastatin ameliorates high glucose-induced injury of cardiomyocytes via increasing the expression of NADPH oxidase mRNA.