中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2009年
6期
455-457
,共3页
樊庆莹%周晓琳%杨赫%陶泽新%李岩%刘桂芳%刘尧%宋立志%张丽%王海岩%徐爱强
樊慶瑩%週曉琳%楊赫%陶澤新%李巖%劉桂芳%劉堯%宋立誌%張麗%王海巖%徐愛彊
번경형%주효림%양혁%도택신%리암%류계방%류요%송립지%장려%왕해암%서애강
脑膜炎%无菌性%肠道病毒B型%人%基因
腦膜炎%無菌性%腸道病毒B型%人%基因
뇌막염%무균성%장도병독B형%인%기인
Meningitis%aseptic%Enterovirus B%human%Genes
目的 明确2008年山东省临沂市郯城县无菌性脑膜炎暴发的病原,对分离到的柯萨奇B3病毒的基因特征、遗传变异规律及进化来源进行分析.方法 采集暴发病例的粪便、脑脊液标本,应用RD、Hep-2细胞进行病毒分离;阳性分离物分别采用中和试验、逆转录-聚合酶链式反应(RT-PCR)和核苷酸序列测定方法进行定型,最后与GenBank中检索到的其他CVB3毒株进行同源性分析,并构建VP1完整编码区亲缘进化树研究其遗传进化规律.结果 共采集22份粪便和120份脑脊液标本,分离到病毒35株,经鉴定34株为CVB3,1株为ECH030.34株CVB3分离株VP1区部分序列(381 bp)核苷酸同源性为90.5%~100%,氨基酸同源性为98.4%~100%;与Nancy原型株的核苷酸同源性为79.5%~81.6%,氨基酸同源性为98.4%~99.2%.012/2008TC/SD/CHN和177/2008TC/SD/CHN与安徽2008年分离的Fuyang19株同源性最高,核苷酸同源性分别为98.2%和91.0%,氨基酸同源性分别为99.2%和98.9%;进化树分析显示,中国大陆分离株独处一支,且呈单源性发生关系.结论 此次脑膜炎暴发的病原是CVB3.它与其他的中国分离株同源性高,与国外分离到的CVB3具有不同的基因特征.
目的 明確2008年山東省臨沂市郯城縣無菌性腦膜炎暴髮的病原,對分離到的柯薩奇B3病毒的基因特徵、遺傳變異規律及進化來源進行分析.方法 採集暴髮病例的糞便、腦脊液標本,應用RD、Hep-2細胞進行病毒分離;暘性分離物分彆採用中和試驗、逆轉錄-聚閤酶鏈式反應(RT-PCR)和覈苷痠序列測定方法進行定型,最後與GenBank中檢索到的其他CVB3毒株進行同源性分析,併構建VP1完整編碼區親緣進化樹研究其遺傳進化規律.結果 共採集22份糞便和120份腦脊液標本,分離到病毒35株,經鑒定34株為CVB3,1株為ECH030.34株CVB3分離株VP1區部分序列(381 bp)覈苷痠同源性為90.5%~100%,氨基痠同源性為98.4%~100%;與Nancy原型株的覈苷痠同源性為79.5%~81.6%,氨基痠同源性為98.4%~99.2%.012/2008TC/SD/CHN和177/2008TC/SD/CHN與安徽2008年分離的Fuyang19株同源性最高,覈苷痠同源性分彆為98.2%和91.0%,氨基痠同源性分彆為99.2%和98.9%;進化樹分析顯示,中國大陸分離株獨處一支,且呈單源性髮生關繫.結論 此次腦膜炎暴髮的病原是CVB3.它與其他的中國分離株同源性高,與國外分離到的CVB3具有不同的基因特徵.
목적 명학2008년산동성림기시담성현무균성뇌막염폭발적병원,대분리도적가살기B3병독적기인특정、유전변이규률급진화래원진행분석.방법 채집폭발병례적분편、뇌척액표본,응용RD、Hep-2세포진행병독분리;양성분리물분별채용중화시험、역전록-취합매련식반응(RT-PCR)화핵감산서렬측정방법진행정형,최후여GenBank중검색도적기타CVB3독주진행동원성분석,병구건VP1완정편마구친연진화수연구기유전진화규률.결과 공채집22빈분편화120빈뇌척액표본,분리도병독35주,경감정34주위CVB3,1주위ECH030.34주CVB3분리주VP1구부분서렬(381 bp)핵감산동원성위90.5%~100%,안기산동원성위98.4%~100%;여Nancy원형주적핵감산동원성위79.5%~81.6%,안기산동원성위98.4%~99.2%.012/2008TC/SD/CHN화177/2008TC/SD/CHN여안휘2008년분리적Fuyang19주동원성최고,핵감산동원성분별위98.2%화91.0%,안기산동원성분별위99.2%화98.9%;진화수분석현시,중국대륙분리주독처일지,차정단원성발생관계.결론 차차뇌막염폭발적병원시CVB3.타여기타적중국분리주동원성고,여국외분리도적CVB3구유불동적기인특정.
Objective To identify the pathogen caused an outbreak of aseptic meningitis in Tancheng county of Shandong province in 2008,and to analyze the molecular characterization of VP1 gene of the Coxsackievirus B3(CVB3)isolates.Methods Stool and cerebrospinal fluid(CSF)specimens were collected from this outbreak for virus isolation with RD and Hep-2 cell.After typing by neutralization test.the VP1 gene of the isolates were amplified by RT-PCR and sequenced.Homologous comparison and phylogenetic analysis were performed.Results 35 strains of enteviruse were isolated from 22 stools and 120 CSFs(7 from stools and 28 from CSFs),34 strains identified as CVB3 and 1 as Echovirus 30(ECHO30)by neutralization test.The nucleotide homologies were 90.5%-100.0% in the partial VP1 gene(381 bp)mnong 34 CVB3 isolates.Homology comparisons indicated that Shandong strains have the identity of 79.5%-81.6% with the CVB3 prototype strain Nancy.012/2008TC/SD/CHN and 177/2008TC/SD,CHN showed the highest nucleotides homologies(98.2% and 91.0% respectively)with Fuyang19 strain of Anhui province in 2008 in complere VP1 gene.The phylogenetic tree based on complete VP1 genes showed that all the CVB3 correlated with aseptic meningitis in China recently came from the same evolution linkage and formed a monophyletic cluster.Conclusion The causative agent of this outhreak of aseptic meningitis wag CVB3.CVB3 circulated in China wag genetically different from other countries.