中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2011年
6期
452-456
,共5页
刘剑仑%杨华伟%陈祖舜%蒋奕
劉劍崙%楊華偉%陳祖舜%蔣奕
류검륜%양화위%진조순%장혁
乳腺肿瘤%核糖体S6蛋白激酶4%逆转录聚合酶链反应%免疫组织化学
乳腺腫瘤%覈糖體S6蛋白激酶4%逆轉錄聚閤酶鏈反應%免疫組織化學
유선종류%핵당체S6단백격매4%역전록취합매련반응%면역조직화학
Breast neoplasms%Ribosomal S6 kinase-4%RT-PCR%Immunohistochemistry
目的 研究核糖体S6蛋白激酶4(RSK-4)在乳腺癌中的表达及意义,探讨RSK-4在乳腺癌发生发展中的作用.方法 应用逆转录聚合酶链反应(RT-PCR)及免疫组织化学染色法,检测RSK-4 mRNA和蛋白在56例乳腺癌组织、癌旁正常乳腺组织和20例乳腺良性病变组织中的表达.结果 RSK-4 mRNA在乳腺癌组织、癌旁正常乳腺组织和乳腺良性病变组织中的阳性表达率分别为48.2%、76.8%和75.0%,RSK-4 mRNA在乳腺癌组织中的阳性表达率明显低于癌旁正常乳腺组织和乳腺良性病变组织(P＜0.05).RSK-4 mRNA在乳腺癌组织中的表达与肿瘤大小和临床分期有关,肿瘤越大、临床分期越晚,RSK-4 mRNA的表达率越低(P＜0.05).RSK-4蛋白在乳腺癌组织、癌旁正常乳腺组织和乳腺良性病变组织中的阳性表达率分别为39.3%、71.4%和75.0%,RSK-4蛋白在乳腺癌组织中的阳性表达率明显低于癌旁正常乳腺组织和乳腺良性病变组织(P＜0.05).RSK-4 蛋白在乳腺癌组织中的表达与肿瘤大小、临床分期以及有无淋巴结转移有关,肿瘤越大、临床分期越晚以及伴淋巴结转移者RSK-4蛋白的表达率低(P＜0.05).56例乳腺癌组织中,RSK-4 mRNA和蛋白的表达一致率为73.2%,二者的表达显著相关(χ2=10.254,P＜0.05).结论 RSK-4可能是乳腺癌的抑癌基因,其表达降低或缺失可能引起乳腺癌的发生和发展.
目的 研究覈糖體S6蛋白激酶4(RSK-4)在乳腺癌中的錶達及意義,探討RSK-4在乳腺癌髮生髮展中的作用.方法 應用逆轉錄聚閤酶鏈反應(RT-PCR)及免疫組織化學染色法,檢測RSK-4 mRNA和蛋白在56例乳腺癌組織、癌徬正常乳腺組織和20例乳腺良性病變組織中的錶達.結果 RSK-4 mRNA在乳腺癌組織、癌徬正常乳腺組織和乳腺良性病變組織中的暘性錶達率分彆為48.2%、76.8%和75.0%,RSK-4 mRNA在乳腺癌組織中的暘性錶達率明顯低于癌徬正常乳腺組織和乳腺良性病變組織(P＜0.05).RSK-4 mRNA在乳腺癌組織中的錶達與腫瘤大小和臨床分期有關,腫瘤越大、臨床分期越晚,RSK-4 mRNA的錶達率越低(P＜0.05).RSK-4蛋白在乳腺癌組織、癌徬正常乳腺組織和乳腺良性病變組織中的暘性錶達率分彆為39.3%、71.4%和75.0%,RSK-4蛋白在乳腺癌組織中的暘性錶達率明顯低于癌徬正常乳腺組織和乳腺良性病變組織(P＜0.05).RSK-4 蛋白在乳腺癌組織中的錶達與腫瘤大小、臨床分期以及有無淋巴結轉移有關,腫瘤越大、臨床分期越晚以及伴淋巴結轉移者RSK-4蛋白的錶達率低(P＜0.05).56例乳腺癌組織中,RSK-4 mRNA和蛋白的錶達一緻率為73.2%,二者的錶達顯著相關(χ2=10.254,P＜0.05).結論 RSK-4可能是乳腺癌的抑癌基因,其錶達降低或缺失可能引起乳腺癌的髮生和髮展.
목적 연구핵당체S6단백격매4(RSK-4)재유선암중적표체급의의,탐토RSK-4재유선암발생발전중적작용.방법 응용역전록취합매련반응(RT-PCR)급면역조직화학염색법,검측RSK-4 mRNA화단백재56례유선암조직、암방정상유선조직화20례유선량성병변조직중적표체.결과 RSK-4 mRNA재유선암조직、암방정상유선조직화유선량성병변조직중적양성표체솔분별위48.2%、76.8%화75.0%,RSK-4 mRNA재유선암조직중적양성표체솔명현저우암방정상유선조직화유선량성병변조직(P＜0.05).RSK-4 mRNA재유선암조직중적표체여종류대소화림상분기유관,종류월대、림상분기월만,RSK-4 mRNA적표체솔월저(P＜0.05).RSK-4단백재유선암조직、암방정상유선조직화유선량성병변조직중적양성표체솔분별위39.3%、71.4%화75.0%,RSK-4단백재유선암조직중적양성표체솔명현저우암방정상유선조직화유선량성병변조직(P＜0.05).RSK-4 단백재유선암조직중적표체여종류대소、림상분기이급유무림파결전이유관,종류월대、림상분기월만이급반림파결전이자RSK-4단백적표체솔저(P＜0.05).56례유선암조직중,RSK-4 mRNA화단백적표체일치솔위73.2%,이자적표체현저상관(χ2=10.254,P＜0.05).결론 RSK-4가능시유선암적억암기인,기표체강저혹결실가능인기유선암적발생화발전.
Objective To study the expression and clinical significance of ribosomal S6 kinase-4 (RSK-4) in breast cancer and explore the role of RSK-4 in the genesis and development of breast cancer. Method The expression levels of RSK-4 mRNA and protein were detected in 56 cases of breast cancer and the normal breast tissues, as well as in 20 cases of breast benign lesions, by reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemistry. Results The expression rates of RSK-4 mRNA in breast cancer, the normal breast tissues and breast benign lesions were 48. 2%, 76. 8% and 75. 0%, respectively. The expression level of RSK-4 mRNA in breast cancer was significantly lower than those in normal breast tissues and breast benign lesions tissues (P<0. 05). The expression level of RSK-4 significantly correlated with tumor size and clinical stage (P<0.05).The expression rate of RSK-4 protein was 39.3% in breast cancer tissues, which was significantly lower than that of normal breast tissues (71.4%) and breast benign lesions (75.0%, P<0.01). The expression level of RSK-4 protein was lower in breast cancer with large tumor, high clinical stage and lymph node metastasis. In 56 cases of breast cancer samples, the consistency rate of RSK-4 mRNA and protein was 73.2%. A significant correlation was found between RSK-4 mRNA and protein (χ2=10.254, P<0.05). Conclusion The down-regulation of RSK-4 expression in breast caner suggests that it is a breast cancer suppressor gene, and the lack or down-regulation of RSK-4 expression is involved in the genesis and progression of breast cancer.
