CAJ | 학술논문

背景:3,4-亚甲基二氧基甲基苯丙胺是苯丙胺类兴奋剂的一种,具有兴奋和致幻作用,对人体的损害也极其严重和复杂.在国外对其研究已成为成瘾行为领域中的热点.目的:观察3,4-亚甲二氧基甲基苯丙胺神经毒性长期效应对认知功能的影响.设计:随机对照动物实验.单位:四川大学华西医院心理卫生中心精神药理实验室.材料:实验于2003-07/2004-02在四川大学华西医院心理卫生中心精神药理实验室完成.随机将四川大学动物中心提供的16只成年雄性Wistar大鼠分为试验组10只,对照组6只.方法:试验组给予3,4-亚甲基二氧基甲基苯丙胺10 mg/kg,1次/h,共4次,即总量为40 mg/kg,对照组给予等体积生理盐水.分别于末次给药后2,8,26,32周进行水迷宫试验观察空间学习记忆功能.主要观察指标:两组大鼠末次给药后第2,8,26,32周时逃避潜伏期和穿原平台所在位置的次数.结果:试验过程中12 h内试验组大鼠死亡4只.6周时试验组大鼠又死1只,17周时对照组大鼠死亡1只,最后实验组、对照组各剩5只直至32周.末次给药后第2,8,26,32周时Morris水迷宫试验,两组逃避潜伏期比较,差异无统计学意义(P＞0.05).两组4个时间点的穿环次数比较,差异也无统计学意义[(7.67±2.16),(7.50±2.95)次;(6.60±1.14),(7.0±1.67)次;(7.40±1.52),(6.60±2.61)次;(6.80±4.55),(5.80±1.79)次,P＞0.05].结论:3,4-亚甲基二氧基甲基苯丙胺神经毒性长期效应对空间学习记忆功能影响不明显.
배경:3,4-아갑기이양기갑기분병알시분병알류흥강제적일충,구유흥강화치환작용,대인체적손해야겁기엄중화복잡.재국외대기연구이성위성은행위영역중적열점.목적:관찰3,4-아갑이양기갑기분병알신경독성장기효응대인지공능적영향.설계:수궤대조동물실험.단위:사천대학화서의원심리위생중심정신약리실험실.재료:실험우2003-07/2004-02재사천대학화서의원심리위생중심정신약리실험실완성.수궤장사천대학동물중심제공적16지성년웅성Wistar대서분위시험조10지,대조조6지.방법:시험조급여3,4-아갑기이양기갑기분병알10 mg/kg,1차/h,공4차,즉총량위40 mg/kg,대조조급여등체적생리염수.분별우말차급약후2,8,26,32주진행수미궁시험관찰공간학습기억공능.주요관찰지표:량조대서말차급약후제2,8,26,32주시도피잠복기화천원평태소재위치적차수.결과:시험과정중12 h내시험조대서사망4지.6주시시험조대서우사1지,17주시대조조대서사망1지,최후실험조、대조조각잉5지직지32주.말차급약후제2,8,26,32주시Morris수미궁시험,량조도피잠복기비교,차이무통계학의의(P＞0.05).량조4개시간점적천배차수비교,차이야무통계학의의[(7.67±2.16),(7.50±2.95)차;(6.60±1.14),(7.0±1.67)차;(7.40±1.52),(6.60±2.61)차;(6.80±4.55),(5.80±1.79)차,P＞0.05].결론:3,4-아갑기이양기갑기분병알신경독성장기효응대공간학습기억공능영향불명현.
BACKGROUND: 3,4-Methylenedioxymethamphetamine (MDMA) is one kind of amphetamine-type stimulants (ATS) with stimulating and hallucinogenic properties, and its damage to human is extremely serious and complicated. It has become a research hot in the field of addiction behavior abroad. OBJECTIVE: To observe the effect of long-term neurotoxicity of MDMA on cognitive function. DESIGN: A randomized control animal experiment. SETTING: Laboratory of Psychopharmacology, Mental Health Center, West China Hospital of Sichuan University. MATERIALS: The experiment was carried out in the Laboratory of Psychopharmacology of the Mental Health Center, West China Hospital of Sichuan University from July 2003 to February 2004. Sixteen male adult Wistar rats, provided by the animal center of Sichuan university, were randomly assigned to study group (n=10) and control group (n= 6). INTERVENTIONS: Rats in study group were administrated with MDMA (10 mg/kg), once per hour for four times, and the total amount was 40 mg/kg, and those in the control group were treated with saline of the same volume. The Morris water-maze test was performed at 2, 8, 26 and 32 weeks after the last administration respectively to observe the spatial learning and memory function. MAIN OUTCOME MEASURES: The escape latencies and the times of crossing the exact position of the former platform were observed at 2, 8, 26 and 32 weeks after the last administration respectively. RESULTS: Four rats in the study group died within 12 hours during the experiment, 1 in the study group and 1 in the control group died at 6 and 17 weeks respectively, finally 5 rats in the study group and control group left till 32 weeks respectively. At 2, 8, 26 and 32 weeks after the last administration, there were no .significant differences in the escape latencies between the two group (P ＞ 0.05), and the times of crossing the exact position of the former platform also had no significant differences [(7.67±2.16), (7.50±2.95) times; (6.60±1.14), (7.0±1.67) times;(7.40±1.52), (6.60±2.61) times; (6.80±4.55), (5.80±1.79) times; P ＞ 0.05]. CONCLUSION: The long-term neurotoxicity of MDMA has no obvious effect on the spatial learning and memory function.