CAJ | 학술논문

背景:孤啡肽与缺血缺氧关系密切,可能在胎儿窘迫、新生儿窒息的发生发展中起到重要作用.目的:观察宫内缺血缺氧胎鼠下丘脑及外周血孤啡肽含量的变化,分析孤啡肽在围生期缺血缺氧中的作用.设计:随机对照动物实验.单位:解放军第二军医大学长海医院妇产科.材料:实验于2002-05/2003-09在第二军医大学长海医院妇产科进行,选择Wistar雌性大鼠12只,平均体质量260 g.方法:将12只雌鼠随机分成3组:对照组、钳夹子宫血管10 min组和钳夹子宫血管20 min组,每组4只.使各组雌鼠受孕,在妊娠第21天,将各组雌鼠剖腹,对照组直接取出胎鼠,产胎鼠19只;钳夹子宫血管10 min组钳夹子宫血管10min后取出胎鼠,产胎鼠21只;钳夹子宫血管20min组钳夹子宫血管20min后取出胎鼠,产胎鼠17只.均成活,所有胎鼠做Apgar评分并迅速断头,收集躯干血并取出全脑,采用放射免疫测定法测定各组胎鼠下丘脑及外周血孤啡肽的含量.主要观察指标:各组胎鼠下丘脑及外周血孤啡肽的含量.结果:57只胎鼠全部进入结果分析.①钳夹子宫血管10min组胎鼠下丘脑及外周血孤啡肽的含量分别为(71±14)pg/g及(31±7)ng/L;钳夹子宫血管20 min组胎鼠下丘脑及外周血孤啡肽的含量分别为(114±21)pg/g及(58±11)ng/L;对照组胎鼠下丘脑及外周血孤啡肽含量分别为(48±9)pg/g及(19±4)ng/L;与对照组相比,钳夹子宫血管10 min和20 min组胎鼠下丘脑及外周血孤啡肽含量均升高(P＜0.05,P＜0.01):且钳夹子宫血管20 min组孤啡肽含量高于钳夹子宫血管10 min组(P＜0.05).②两组胎鼠Apgar评分均低于对照组(P＜0.01),其中钳夹子宫血管20min组胎鼠Apgar评分低于钳夹子宫血管10min组(P＜0.05).结论:围生期缺血缺氧可导致下丘脑及外周血孤啡肽的含量增加.
배경:고배태여결혈결양관계밀절,가능재태인군박、신생인질식적발생발전중기도중요작용.목적:관찰궁내결혈결양태서하구뇌급외주혈고배태함량적변화,분석고배태재위생기결혈결양중적작용.설계:수궤대조동물실험.단위:해방군제이군의대학장해의원부산과.재료:실험우2002-05/2003-09재제이군의대학장해의원부산과진행,선택Wistar자성대서12지,평균체질량260 g.방법:장12지자서수궤분성3조:대조조、겸협자궁혈관10 min조화겸협자궁혈관20 min조,매조4지.사각조자서수잉,재임신제21천,장각조자서부복,대조조직접취출태서,산태서19지;겸협자궁혈관10 min조겸협자궁혈관10min후취출태서,산태서21지;겸협자궁혈관20min조겸협자궁혈관20min후취출태서,산태서17지.균성활,소유태서주Apgar평분병신속단두,수집구간혈병취출전뇌,채용방사면역측정법측정각조태서하구뇌급외주혈고배태적함량.주요관찰지표:각조태서하구뇌급외주혈고배태적함량.결과:57지태서전부진입결과분석.①겸협자궁혈관10min조태서하구뇌급외주혈고배태적함량분별위(71±14)pg/g급(31±7)ng/L;겸협자궁혈관20 min조태서하구뇌급외주혈고배태적함량분별위(114±21)pg/g급(58±11)ng/L;대조조태서하구뇌급외주혈고배태함량분별위(48±9)pg/g급(19±4)ng/L;여대조조상비,겸협자궁혈관10 min화20 min조태서하구뇌급외주혈고배태함량균승고(P＜0.05,P＜0.01):차겸협자궁혈관20 min조고배태함량고우겸협자궁혈관10 min조(P＜0.05).②량조태서Apgar평분균저우대조조(P＜0.01),기중겸협자궁혈관20min조태서Apgar평분저우겸협자궁혈관10min조(P＜0.05).결론:위생기결혈결양가도치하구뇌급외주혈고배태적함량증가.
BACKGROUND: Orphanin (OFQ) is associated with ischemia/hypoxia,which may play an important role in the production and development of fetal distress and neonatal asphyxia.OBJECTIVE: To observe the change of OFQ content in hypothalamus and peripheral blood of intrauterine ischemia/hypoxia fetal rats and analyze the role of OFQ in the perinatal ischemia/hypoxia.DESIGN: Randomized controlled animal trial.SETTING: Department of Obstetrics and Gynecology, Changhai Hospital of the Second Military Medical University of Chinese PLA.MATERIALS: The experiment was performed at the Department of Obstetrics and Gynecology, Changhai Hospital of the Secon d Military Medical University of Chinese PLA from May 2002 to September 2003. A total of 12 Wistar female rats, with the mean body mass of 260 g were selected and fed routinely [provided by the experiment animal center of this university, number of certificate scxk(Hu)2002/0006].METHODS: The 12 female rats were randomized into three groups: control group, ischemia/hypoxia for 10 minutes group, ischemia/hypoxia for 20 minutes group with 4 rats in each group. Female rats in each group were pregnant. On day 21 of pregnancy, female rats in each group were cut the belly open, and the uterine vessels were incarcerated for 10 minutes in the ischemia/hypoxia for 10 minutes group with 21 fetal rats and 20 minutes in the ischemia/hypoxia for 20 minutes group with 17 fetal rats, respectively. Fetal rats were directly obtained from control group with 19 ones. None of fetal rats died. All the fetal rats received Apgar score and decapitation. The blood of trunk was collected and the whole brain was obtained. OFQ content in hypothalamus and peripheral blood was measured with radioimmunoassay.MAIN OUTCOME MEASURES: OFQ content in hypothalamus and peripheral blood of fetal rats in each group.RESULTS: Totally 57 rats were involved in the result analysis. ①The levels ofOFQ in hypothalamus and peripheral blood were (71±14) pg/g and (31±7) ng/L in ischemia/hypoxia for 10 minutes group, (114±21) pg/g and (58±11) ng/L in ischemia/hypoxia for 20 minutes group, (48±9) pg/g and (19±4) ng/L in the control group. Compared with the control group, the levels of OFQ in hypothalamus and peripheralblood increased in the ischemia/hypoxia for 10 minutes group and ischemia/hypoxia for 20 minutes group (P＜ 0.05, P ＜ 0.01), and it in ischemia/hypoxia for 10 minutes group was significantly higher than that in ischemia/hypoxia for 20 minutes group (P ＜ 0.05). ②The score of Apgar was lower in the two groups than in the control group (P ＜ 0.01 ), of which it was lower in the ischemia/hypoxia for 20minutes group than in the ischemia/hypoxia for 10 minutes group (P ＜ 0.05).CONCLUSION: The perinatal ischemia and hypoxia can induce the increase of OFQ content in hypothalamus and peripheral blood.