CAJ | 학술논문

[目的]研究影响鸡骨草愈伤组织形成的条件,为鸡骨草的组织培养及悬浮培养提供参考.[方法]从鸡骨草的大田植株和种子发芽后的幼芽中合适部位选取外植体,在附加不同浓度、不同组合的外源激素2,4-D和6-BA的MS培养基上,对鸡骨草愈伤组织进行诱导,计算诱导率,观察愈伤组织生长情况.[结果]单独使用一种激素时对愈伤组织的诱导能力大致相同,2,4-D比6-BA对鸡骨草茎段愈伤组织的诱导能力稍强一些.培养基中添加2种激素时愈伤组织褐变程度稍轻,最适合的培养基为MS附加2,4-D 2.0 mg/L+6-BA 1.5 mg/L.[结论]多种激素组合比单种激素有利于愈伤组织的形成,培养基中适宜的激素组合可降低愈伤组织褐变率.
[목적]연구영향계골초유상조직형성적조건,위계골초적조직배양급현부배양제공삼고.[방법]종계골초적대전식주화충자발아후적유아중합괄부위선취외식체,재부가불동농도、불동조합적외원격소2,4-D화6-BA적MS배양기상,대계골초유상조직진행유도,계산유도솔,관찰유상조직생장정황.[결과]단독사용일충격소시대유상조직적유도능력대치상동,2,4-D비6-BA대계골초경단유상조직적유도능력초강일사.배양기중첨가2충격소시유상조직갈변정도초경,최괄합적배양기위MS부가2,4-D 2.0 mg/L+6-BA 1.5 mg/L.[결론]다충격소조합비단충격소유리우유상조직적형성,배양기중괄의적격소조합가강저유상조직갈변솔.
[Objective] The study aimed to research the condition of influencing the callus formation of Abrus cantoniensis Hance and provide the reference for the tissue culture and suspend culture of A. cantoniensis. [Method] The explants were selected from the suitable position of the plants in the field and the young buds after the seed germination of A. cantoniensis. The calli of A. cantoniensis were induced on the MS media added by the exogenous hormone of 2,4-D and 6-BA with different concn. and different combinations. the inducing rate was calculated and the growth situation of calli was observed. [Result] Using one kinds of hormone singly had a nearly same ability on inducing the calli of A. cantoniensis and 2,4-D showed a slight strong ability on inducing the calli from the stem segment of A. cantoniensis compared with 6-BA. When the medium was added by 2 kinds of hormone, the browning degree of the calli become slight. The most suitable medium was MS medium added by the growth regulators of 2,4-D 2.0 mg/L+6-BA 1.5 mg/L.[Conclusion] Compared with the single hormone, many kinds of hormone combinations were propitious to the callus formation of A. cantoniensis and the suitable hormone combinations added on the medium could reduce the browning rate of the calli.