CAJ | 학술논문

番茄中含有番茄红素和β-胡萝卜素两种重要的营养成分.两种成分的主要拉曼光谱基团相同,利用基频很难在体将两种成分区分开.应用激发波长为514.5 nm的激发光恰好处在番茄红素和β-胡萝卜素主要吸收带的半高宽范围内,因此能够发生共振拉曼效应.利用共振拉曼光谱技术,通过在体测量番茄中的番茄红素和β-胡萝卜素C=C碳碳共轭双键伸缩振动的二次谐波,用软件获得每种成分光谱的积分强度而得到番茄红素和β-胡萝卜素的含量,为在体检测同种基团成分含量提供一种方法.
번가중함유번가홍소화β-호라복소량충중요적영양성분.량충성분적주요랍만광보기단상동,이용기빈흔난재체장량충성분구분개.응용격발파장위514.5 nm적격발광흡호처재번가홍소화β-호라복소주요흡수대적반고관범위내,인차능구발생공진랍만효응.이용공진랍만광보기술,통과재체측량번가중적번가홍소화β-호라복소C=C탄탄공액쌍건신축진동적이차해파,용연건획득매충성분광보적적분강도이득도번가홍소화β-호라복소적함량,위재체검측동충기단성분함량제공일충방법.
Lyeopene and fl-carotene are two important nutritional components in tomato. The main Raman spectrum group of ly-copene and β-carotene abundant in tomato is identical and difficult to be distinguished through fundamental frequency. With exci-tation wavelength of 514. 5 nm, the excited light was just present in the half width range of the main absorption bands of Lyco-pene and β-carotene, so the resonance Raman effect can occur. Based on resonance Raman spectra, by on-body measuring the second harmonic of stretching vibration of carbon-carbon conjugated double bond in lycopene and β-carotene, the content of lyco-pene and β-carotene can be obtained according to the integrated intensity of each component calculated by software. And this pro-vides a method for on-body determining the content of the components with the homologous group.