CAJ | 학술논문

目的研究浙江省慈溪市啮齿动物中汉坦病毒(HV)流行情况及病毒型别.方法采用间接免疫荧光法(IFA)检测鼠肺中HV抗原;用逆转录聚合酶链反应(RT-PCR)法扩增阳性样品中HV的部分S片段;构建系统发生树进行系统发生分析及分型.结果在慈溪市肾综合征出血热(HFRS)疫点共捕获啮齿动物243只,在7份鼠肺样品中检测到HV抗原,其中4只为褐家鼠,3只为黄胸鼠,病毒携带率为2.88%.用汉城型病毒(SEOV)特异性引物从6份HV抗原阳性样品中扩增出部分S片段(620～999 nt)并测定了序列.对扩增出的部分S片段核苷酸序列分析表明,6株病毒与现有的SEOV有最高的同源性,均为SEOV.用部分S片段核苷酸序列所构建的系统进化树显示,6株病毒构成两个单元支,其中由褐家鼠携带的3株病毒与BjHD01株病毒的亲缘关系最近,分在同一分支,由黄胸鼠携带的3株病毒构成另一分支,与L99、R22及Hb8610株的亲缘关系较近.结论慈溪市的褐家鼠与黄胸鼠分别携带一种不同亚型的SEOV,表现出SEOV在同一地区的遗传多样性,并支持宿主与汉坦病毒共进化的理论.
목적 연구절강성자계시교치동물중한탄병독(HV)류행정황급병독형별.방법 채용간접면역형광법(IFA)검측서폐중HV항원;용역전록취합매련반응(RT-PCR)법확증양성양품중HV적부분S편단;구건계통발생수진행계통발생분석급분형.결과 재자계시신종합정출혈열(HFRS)역점공포획교치동물243지,재7빈서폐양품중검측도HV항원,기중4지위갈가서,3지위황흉서,병독휴대솔위2.88%.용한성형병독(SEOV)특이성인물종6빈HV항원양성양품중확증출부분S편단(620～999 nt)병측정료서렬.대확증출적부분S편단핵감산서렬분석표명,6주병독여현유적SEOV유최고적동원성,균위SEOV.용부분S편단핵감산서렬소구건적계통진화수현시,6주병독구성량개단원지,기중유갈가서휴대적3주병독여BjHD01주병독적친연관계최근,분재동일분지,유황흉서휴대적3주병독구성령일분지,여L99、R22급Hb8610주적친연관계교근.결론 자계시적갈가서여황흉서분별휴대일충불동아형적SEOV,표현출SEOV재동일지구적유전다양성,병지지숙주여한탄병독공진화적이론.
Objective To collect more data on the epidemiology of hantavirus in rodents in Cixi,Zhejiang province.Methods Rodents were captured in Cixi,where hemorrhagic fever with renal syndrome (HFRS)appeared endemic.Hantavirus antigens in the rat lungs were detected by immunofluorescence assay.Partial S segment sequences were amplified by reverse transciption-polymerase chain reaction(RTPCR)and then sequenced.The phyologenetic trees were constructed by maximum likelihood method to detect the genetic characteristics of hantavirus.Results A total of 243 rodents were trapped in the epidemic areas,and hantavirus antigens were identified in 7 out of these lung samples(2.88%).Partial S segment sequences(620-999 nt)were recovered from 6 samples and sequenced.Data from phylogenetic analysis of these S segment sequences indicated that all viruses belonged to Seoul virus(SEOV),despite the origins of sources were either from Rattus norvegicus or from R.fzabipectus.These viruses could further be divided into two distinct lineages but the viruses carried by R.norvegicus were different from those carried by R.flabipectus.Conclusion Two distinct lineages of SEOV had been cocirculating in rodents in Cixi.