CAJ | 학술논문

目的评价不同级别医院放射工作人员的DNA损伤情况,探讨不同工种、工龄与DNA损伤程度之间的关系.方法用碱性单细胞凝胶电泳技术检测放射组和对照组外周血淋巴细胞的DNA单链断裂水平.CASP软件分析彗星图像,主要观察彗星尾部DNA百分含量(TDNA%)、彗星尾长(TL)、尾矩(TM)和Olive尾矩(OTM)等指标.结果放射组的TDNA%,TL、TM、OTM明显高于对照组(F=3.93.P<0.01),不同工种、工龄间均有统计学差异(F=1.83,P<0.05),不同级别医院之间放射工作者上述指标也差异存在统计学意义(F=1.91,P<0.05).结论所观察医院的放射工作者外周血淋巴细胞DNA均存在不同程度的损伤,高级别医院略好于低级别医院,而且DNA损伤程度与放射工龄和工种有一定的相关性.
목적 평개불동급별의원방사공작인원적DNA손상정황,탐토불동공충、공령여DNA손상정도지간적관계.방법 용감성단세포응효전영기술검측방사조화대조조외주혈림파세포적DNA단련단렬수평.CASP연건분석혜성도상,주요관찰혜성미부DNA백분함량(TDNA%)、혜성미장(TL)、미구(TM)화Olive미구(OTM)등지표.결과 방사조적TDNA%,TL、TM、OTM명현고우대조조(F=3.93.P<0.01),불동공충、공령간균유통계학차이(F=1.83,P<0.05),불동급별의원지간방사공작자상술지표야차이존재통계학의의(F=1.91,P<0.05).결론 소관찰의원적방사공작자외주혈림파세포DNA균존재불동정도적손상,고급별의원략호우저급별의원,이차DNA손상정도여방사공령화공충유일정적상관성.
objective To assess the DNA damage of radiation workers in different grade hospitals,and to explore the correlation between the types of work or work time and the levels of DNA damage.Methods DNA single strand break were detected by using alkaline single cell gel electrophoresis(SCGE),and the comet was analyzed with CASP(Comet Assay Software Project).TDNA,TL,TM and OTM were calculated.Results The parameters of SCGE in the radiation greup were higher than those of control group(F=3.93,P<0.01).The significant difference was found not only among the different types of work or difierent work time,but also among the different grade hospitals(F=1.83,1.9 1,P<0.05).Conclusions Various levels of DNA damage could be detected in the radiation workers of the two hospitals.DNA damage of radiation workers is less serious in the higher-grade hospital than the lower grade one.Different types of work or work time might affect the DNA damage level.