CAJ | 학술논문

研究小鼠红细胞分化去核分化因子(MEDDF)在红细胞终末分化中的功能。方法构建在真核细胞中表达的重组质粒pcDNA-MEDDF，用其转染小鼠红白血病细胞系(MEL)。通过分析细胞的生长曲线、分裂指数及在半固体培养基中的集落形成率比较细胞的生长特性，采用RT-PCR检测c-myc及β-珠蛋白(β-globin)基因的表达。结果转染pcDNA-MEDDF与转染空载体(pcDNA3.1)的细胞相比较，细胞的增殖率减慢、分裂指数降低、在半固体培养基中形成集落的数量减少。转染细胞的联苯胺阳性率达32.8％。用RT-PCR检测发现，转染细胞β-珠蛋白的表达量上升了3.43倍，c-myc表达量下降了66.3％。结论 MEDDF能使小鼠红白血病细胞分化并抑制其恶性变。
연구소서홍세포분화거핵분화인자(MEDDF)재홍세포종말분화중적공능。방법구건재진핵세포중표체적중조질립pcDNA-MEDDF，용기전염소서홍백혈병세포계(MEL)。통과분석세포적생장곡선、분렬지수급재반고체배양기중적집락형성솔비교세포적생장특성，채용RT-PCR검측c-myc급β-주단백(β-globin)기인적표체。결과전염pcDNA-MEDDF여전염공재체(pcDNA3.1)적세포상비교，세포적증식솔감만、분렬지수강저、재반고체배양기중형성집락적수량감소。전염세포적련분알양성솔체32.8％。용RT-PCR검측발현，전염세포β-주단백적표체량상승료3.43배，c-myc표체량하강료66.3％。결론 MEDDF능사소서홍백혈병세포분화병억제기악성변。
Objective To investigate the roles of mouse erythroid differentiation and denucleation factor (MEDDF), newly cloned in our laboratory, in erythroid terminal differentiation. Methods Mouse erythroleukemia cells (MEL) were transfected with eukaryotic expression plasmid pcDNA-MEDDF. The changes of cell growth rate, mitotic index and colony-forming rate in semi-solid medium were investigated.The expressions of c-myc and β-globin genes were analysed by semi-quantitative RT-PCR. Results MEL cells transfected with pcDNA-MEDDF showed significant lower growth rate, mitotic index, and colony-forming rate in semisolid medium(P＜0.01).The percentage of benzidine-positive cells was 32.8% after transfection. The expression of β-globin in cells transfected with pcDNA-MEDDF was 3.43 times higher than that of control (MEL transfected with blank vector, pcDNA3.1 ), and the expression of c-myc was decreased by 66.3%.Conclusions MEDDF can induce differentiation of MELcell, and suppress its malignancy likely.