CAJ | 학술논문

目的研究硒、VitC、Vit E、β-胡萝卜素联用对小鼠细胞免疫功能及抗氧化功能的调节作用。方法随机分成五组：对照组、剂量A组、B组、免疫抑制组、免疫抑制＋A组。每组小鼠分别喂饲不同剂量复合营养素，并测定它们的免疫功能和抗氧化功能。结果研究发现每天补充A剂量复合营养素(Se 1μg、VitC 1.2mg、VitE 0.24mg、β-胡萝卜素0.1mg)显著增强小鼠的淋巴细胞转化功能，增强脾细胞对IL-2的反应活性以及提高NK细胞活性。超过一定的剂量(Se 5μg、VitC 6mg、VitE 1.2mg、β-胡萝卜素0.5mg)则表现出明显的免疫抑制作用(P＜0.01)。研究还发现Se、VitC、VitE、β-胡萝卜素联用在一定剂量内能对抗免疫抑制剂(环磷酰胺)的作用，刺激免疫功能低下的小鼠脾细胞产生IL-2，增强淋巴细胞的转化功能，提高NK细胞活性，恢复受抑制的淋巴细胞产生IL-2的能力。实验表明抗氧化营养素有双向的免疫调节作用。同时补硒可使小鼠肌肉及血清硒含量上升，补充抗氧化营养素可使小鼠血清及肝LPO浓度降低，肝SOD浓度升高。结论一定剂量的Se、ⅥtC、VitE、β-胡萝卜素联用可使小鼠的免疫及抗氧化功能提高。
목적연구서、VitC、Vit E、β-호라복소련용대소서세포면역공능급항양화공능적조절작용。방법수궤분성오조：대조조、제량A조、B조、면역억제조、면역억제＋A조。매조소서분별위사불동제량복합영양소，병측정타문적면역공능화항양화공능。결과연구발현매천보충A제량복합영양소(Se 1μg、VitC 1.2mg、VitE 0.24mg、β-호라복소0.1mg)현저증강소서적림파세포전화공능，증강비세포대IL-2적반응활성이급제고NK세포활성。초과일정적제량(Se 5μg、VitC 6mg、VitE 1.2mg、β-호라복소0.5mg)칙표현출명현적면역억제작용(P＜0.01)。연구환발현Se、VitC、VitE、β-호라복소련용재일정제량내능대항면역억제제(배린선알)적작용，자격면역공능저하적소서비세포산생IL-2，증강림파세포적전화공능，제고NK세포활성，회복수억제적림파세포산생IL-2적능력。실험표명항양화영양소유쌍향적면역조절작용。동시보서가사소서기육급혈청서함량상승，보충항양화영양소가사소서혈청급간LPO농도강저，간SOD농도승고。결론일정제량적Se、ⅥtC、VitE、β-호라복소련용가사소서적면역급항양화공능제고。
Objective Effects of a combined supplementation with selenium、vitamins C、E、β-carotene on the immunity and antioxidation of mice were studied. Methods 50 mice were divided into five groups, and each group received a different dose of antioxidants for 20 days. Results It was found that the immune-normal mice feeded into dose A (Se 1μg、β-carotene 0.1mg, VitE 0.24mg, VitC 1.2mg) showed obvious enhancement in lymphocyte proliferation and activity of Natural killer (NK) cells and IL-2 (P＜0.01). When the dose increased 5 times of the dose A, the immunity of the mice was significantly lower than the control group (P＜0.01). The models of immune-suppressed mice were prepared by intra- peritoneal injection of cyclophosphamide. It was found that the immune-suppressed mice feeded dose A showed obviously enhancement in lymphocyte proliferation and greater NK cell and IL-2 activity, and liver LPO was correlated with serum IL-2 activity. Conclusion Supplementation with dose A can enhance immunity and antioxidation in mice.