CAJ | 학술논문

目的:确定白花丹参的最佳采收期.方法:每半月采挖白花丹参15株,除去根部泥土杂质,自然晒干后计算根单株干重平均值.采用Wondasil-C18色谱柱(150mm×4.6mm,5μm),乙腈-水(74:26)和甲醇-乙腈-甲酸-水(30:10:1:59)分别作为丹参酮ⅡA和丹酚酸B分离的流动相,流速均为1.0mL·min-1,用高效液相色谱法在270nm和286nm处分别测定丹参酮ⅡA和丹酚酸B的含量.结果:白花丹参10月底采收干重最大,丹参酮ⅡA含量在10月底采收时最高,丹酚酸B含量在8月底采收时最高.结论:白花丹参最佳采收期以10月底为最佳.
목적:학정백화단삼적최가채수기.방법:매반월채알백화단삼15주,제거근부니토잡질,자연쇄간후계산근단주간중평균치.채용Wondasil-C18색보주(150mm×4.6mm,5μm),을정-수(74:26)화갑순-을정-갑산-수(30:10:1:59)분별작위단삼동ⅡA화단분산B분리적류동상,류속균위1.0mL·min-1,용고효액상색보법재270nm화286nm처분별측정단삼동ⅡA화단분산B적함량.결과:백화단삼10월저채수간중최대,단삼동ⅡA함량재10월저채수시최고,단분산B함량재8월저채수시최고.결론:백화단삼최가채수기이10월저위최가.
Objective: To research the optimal harvesting time of Salvia miltiorrhiza Bge. F. alba. Methods: 15 Salvia miltiorrhiza Bge. F. alba were excavated per half month with soil and impurities removed. Average dry weight per plant was calculated after root naturally dried. The HPLC was carried out to determine the contents of tanshinone and salvianolic acid B by Wondasil-C18column( 150mm ×4. 6mm,5μm) with wavelength detection of tanshinoneⅡA for 270 run, salvianolic acid B for 286nm. The mobile phase was 74％ acetonitrile in seperating tanshinoneⅡA, methanol-acetoni-trile-formic acid-water (30:10:1:59) in seperating salvianolic acid B. The flow rate was 1.0mL·min-1. Results: The re-suits showed that there was the greatest dry weight of Salvia miltiorrhiza Bge. F. alba .which was collected by the end of October, the contents of tanshinone ⅡA was the highest by the end of October,while the salvianolie acid B contents in the highest by the end of August. Conclusion: It was suitable for harvesting time of Salvia miltiorrhiza Bge. F. alba by the end of October.