CAJ | 학술논문

试验研究了在成熟液中分别添加0.1% PVA、10% FBS和不同浓度的EGF(20、30、40、50 ng·mL~(-1))对猪卵母细胞体外成熟的影响,以确立猪卵母细胞体外成熟的最佳务件.结果表明,在成熟液中添加0.1%PVA和10% FBS后,对猪卵母细胞的成熟率影响无显著差异(P＞0.05),对猪卵母细胞孤雌激活后36 h的卵裂率和第6天的桑葚胚率无显著差异(P＞0.05);添加不同浓度的EGF对猪卵母细胞体外成熟率无显著差异(P＞0.05);但是添加30和40 ng·mL~(-1) EGF组在36 h的卵裂率要极显著地高于添加20和50ng·mL~(-1) EGF的两组(P＜0.01);而分别添加30、40和50 ng·mL~(-1) EGF 3组处理在第6天的桑葚胚率无显著差异,且极显著地高于添加20 ng·mL~(-1) EGF组的(P＜0.01).所以,在成熟液中添加0.1% PVA可以替代10% FBS.尤以添加30 ng·mL~(-1) EGF组对猪卵母细胞的体外成熟效果较好.
시험연구료재성숙액중분별첨가0.1% PVA、10% FBS화불동농도적EGF(20、30、40、50 ng·mL~(-1))대저란모세포체외성숙적영향,이학립저란모세포체외성숙적최가무건.결과표명,재성숙액중첨가0.1%PVA화10% FBS후,대저란모세포적성숙솔영향무현저차이(P＞0.05),대저란모세포고자격활후36 h적란렬솔화제6천적상심배솔무현저차이(P＞0.05);첨가불동농도적EGF대저란모세포체외성숙솔무현저차이(P＞0.05);단시첨가30화40 ng·mL~(-1) EGF조재36 h적란렬솔요겁현저지고우첨가20화50ng·mL~(-1) EGF적량조(P＜0.01);이분별첨가30、40화50 ng·mL~(-1) EGF 3조처리재제6천적상심배솔무현저차이,차겁현저지고우첨가20 ng·mL~(-1) EGF조적(P＜0.01).소이,재성숙액중첨가0.1% PVA가이체대10% FBS.우이첨가30 ng·mL~(-1) EGF조대저란모세포적체외성숙효과교호.
The effect of 0.1% PVA,10% FBS and different concentration of EGF in the maturation media on maturation of porcine oocytes was studied. The results showed that : Addition of 0.1% PVA and 10% FBS in maturation medium had no significant effect on maturation rate,cleavage rate of 36~(th) h and 6~(th) day morula rate (P>0.05); Addition of different concentration of EGF in maturation medium had no significant effect on maturation rate (P>0.05),but addition of 30 and 40 ng·mL~(-1) EGF had higher cleavage rate of 36~(th) h compared with that of 20 and 50 ng·mL~(-1)(P<0.01),addition of 30,40 and 50 ng·mL~(-1) EGF in maturation medium had no significant effect on 6~(th) day morula rate(P>0.05),and it was significantly higher than 20ng>mL~(-1) (P<0.01). In conclusion,addition of 0.1% PVA could instead of 10% FBS in maturation medium,addition of 30ng·mL~(-1) EGF was the best for maturation of porcine oocytes and development of parthenogenetic embryoes.