CAJ | 학술논문

目的:研究艾灸疗法对快速老化模型(SAMP8)小鼠海马神经干细胞(NSCs)分化的影响.方法:24只SAMP8小鼠随机分为模型组与艾灸组,12只抗快速老化型(SAMR1)小鼠作为正常对照组,艾灸组选用"百会"穴进行艾灸治疗.每天治疗1次,7d为1个疗程,共治疗3个疗程.处死前1周开始给予小鼠50mg/kg的溴脱氧尿嘧啶核苷(BrdU)腹腔注射,治疗结束后,取海马组织,用免疫荧光双标方法检测NSCs分化.结果:①各组小鼠海马均有新生神经元免疫荧光双标阳性细胞表达.与模型组比较.艾灸组有促进或诱导NSCs向成熟神经元及未成熟神经元分化的倾向(P>0.05).②模型组成熟星形胶质细胞神经胶质纤维酸性蛋白表达增多,艾灸能使其表达下降(P>0.05);模型组未成熟星形胶质细胞表达减少,艾灸能促进其表达(P<O.05).③与对照组比较,模型组少突胶质细胞增多(P<O.05);艾灸能减少其表达(P<0.05).结论:经过3个疗程的艾灸治疗,能抑制小鼠海马NSCs向少突胶质细胞分化,促进其向未成熟星形胶质分化,同时其也有向神经元分化倾向.
목적:연구애구요법대쾌속노화모형(SAMP8)소서해마신경간세포(NSCs)분화적영향.방법:24지SAMP8소서수궤분위모형조여애구조,12지항쾌속노화형(SAMR1)소서작위정상대조조,애구조선용"백회"혈진행애구치료.매천치료1차,7d위1개료정,공치료3개료정.처사전1주개시급여소서50mg/kg적추탈양뇨밀정핵감(BrdU)복강주사,치료결속후,취해마조직,용면역형광쌍표방법검측NSCs분화.결과:①각조소서해마균유신생신경원면역형광쌍표양성세포표체.여모형조비교.애구조유촉진혹유도NSCs향성숙신경원급미성숙신경원분화적경향(P>0.05).②모형조성숙성형효질세포신경효질섬유산성단백표체증다,애구능사기표체하강(P>0.05);모형조미성숙성형효질세포표체감소,애구능촉진기표체(P<O.05).③여대조조비교,모형조소돌효질세포증다(P<O.05);애구능감소기표체(P<0.05).결론:경과3개료정적애구치료,능억제소서해마NSCs향소돌효질세포분화,촉진기향미성숙성형효질분화,동시기야유향신경원분화경향.
Objective:To investigate the influence of moxibustion therapy on hippocampal neural stem cells(NFCs) differentiation of senescence-accelerated mouse prone 8(SAMP8) mice. Method:Twenty-four SAMP8 mice were randomized equally into model group and moxibustion treatment group, with 12 senescence-accelerated mouse/resistance (SAMR1) mice as control group. Moxibustion on Baihui (Du20) was administered once daily for 21 consecutive days in mice in moxibustion group.After i.p bromodeoxyuridine(BrdU) 50mg/kg, mice were sacrificed 1 week later, then the proliferation and differentiation of NSCs in hippocampus were detected by immunohistochemisby,double-labeled immunofluorescent. Result: ① New neurons appeared in both SAMP8 and SAMR1 groups. Compared to SAMP8 group, the expression of Brdlf/MAP-2(microtube associated protein-2) and BrdU/B-tubulin Ⅲ in moxibustion group had no obvious change and only showed increasing tendency (P>0.05). ② Compared to SAMR1 group, the expression of BrdU/GFAP (glialfibrillary acidic protein) increased in SAMP8 group, and decreased in moxibustion group (P>0.05). The positive cells of BrdU/ S-100 B decreased in SAMP8 group, and increased in moxibustion group (P<0.05).③Compared to SAMR1 group,the positive cells of BrdU/GalC increased significantly (P<0.05) in SAMP8 group; after treatment of moxibustion, the expression decreased (P<0.05). Conclusion: After moxibustion treatment for 21d, it can inhibit the hippocampal NSCs' differentiating into oligodendrocyte and promote differentiating into immaturity astrocytes, but it also has the tendency of differentiating into neurons.