CAJ | 학술논문

目的:检测金银花提取物对小鼠肝脏和L02细胞中过氧化物酶体增殖物激活受体γ(PPART)共激活因子-1αPGC-1α表达及对胰岛素抵抗(IR)的影响.方法:分别将ob/ob小鼠、C57/B6J小鼠和L02细胞随机分为对照组和实验组,实验组给予金银花提取物,对照组给予生理盐水.给药后检测小鼠肝脏和L02细胞PGC-1α表达.结果:与对照组相比,实验组ob/ob小鼠给予金银花提取物后肝脏PGC-1α mRNA和蛋白表达下调(P<0.05或P<0.01);而实验组C57/B6J小鼠给药后肝脏PGC-1α mRNA表达与对照组比较无统计学差异.给药5d后实验组ob/ob小鼠FPG降低,但实验组与对照组组间胰岛素水平无统计学差异,实验组FPG/FIns比值显著降低.胰岛素耐量实验显示金银花提取物改善了ob/ob小鼠的IR.脂肪酸培养L02细胞,给予金银花提取物后PGC-1α mRNA表达下调(P<0.05),而正常培养L02细胞PGC-1αmRNA表达无变化.结论:金银花提取物可以下调PGC-1α在肝脏中的病理性高表达,降低血糖,改善IR.
목적:검측금은화제취물대소서간장화L02세포중과양화물매체증식물격활수체γ(PPART)공격활인자-1αPGC-1α표체급대이도소저항(IR)적영향.방법:분별장ob/ob소서、C57/B6J소서화L02세포수궤분위대조조화실험조,실험조급여금은화제취물,대조조급여생리염수.급약후검측소서간장화L02세포PGC-1α표체.결과:여대조조상비,실험조ob/ob소서급여금은화제취물후간장PGC-1α mRNA화단백표체하조(P<0.05혹P<0.01);이실험조C57/B6J소서급약후간장PGC-1α mRNA표체여대조조비교무통계학차이.급약5d후실험조ob/ob소서FPG강저,단실험조여대조조조간이도소수평무통계학차이,실험조FPG/FIns비치현저강저.이도소내량실험현시금은화제취물개선료ob/ob소서적IR.지방산배양L02세포,급여금은화제취물후PGC-1α mRNA표체하조(P<0.05),이정상배양L02세포PGC-1αmRNA표체무변화.결론:금은화제취물가이하조PGC-1α재간장중적병이성고표체,강저혈당,개선IR.
Objective To explore the effects of extracts of Lonicera japonica Thunb. on hepatic PGC-1α expression and insulin resistance in mice and L02 cell. Methods C57/B6J mice were divided into control group (group A)and Lonicera japonica Thunb.-treated group (group B). The ob/ob mice were divided into control group (group C)and Lonicera japonica Thunb.-treated group (group D). L02 cells cultured in normal medium were divided into control group (group a) and Lonicera japonica Thunb.-treated group(group b). L02 cells cultured in medium with FFA (free fatty acid) were divided into control group(group c)and Lonicera japonica Thunb.-treated group (group d). The basal blood glucose and insulin were measured and insulin tolerance test(ITT) was done in ob/ob mice．PGC-1α mRNA and protein expressions were detected by q-RT-PCR and Western blot respectively. Results Hepatic mRNA expression of PGC-1α were significantly decreased in group d versus group c(P<0.05) and in group D versus group C(P<0.05) , while were similar in group b versus group a and in group B versus group A (all P＞0.05)．Hepatic protein expression of PGC-1α was significantly lower in group D than in group C(P<0.01). Basal blood glucose were significantly lower in group D than in group C (P<0.05), while insulin secretion were similar (P＞0.05). Hepatic insulin resistance in group D was improved according to the results of ITT. Conclusions Lonicera japonica Thunb. decreases the pathologically high expression of PGC-1α mRNA in liver and basal blood glucose, and improves insulin resistance .