国际医学寄生虫病杂志
國際醫學寄生蟲病雜誌
국제의학기생충병잡지
INTERNATIONAL JOURNAL OF MEDICAL PARASITIC DISEASES
2008年
3期
162-166
,共5页
隐孢子虫%检测方法%介水传播
隱孢子蟲%檢測方法%介水傳播
은포자충%검측방법%개수전파
Cryptosporidium spp.%Detection method%Waterborne transmission
隐孢子虫(Cryptosporidium spp.)是一类顶复门肠道原虫,隐孢子虫病(cryptosporidiosis)是由隐孢子虫引起的人兽共患原虫病.由于隐孢子虫病可通过水传播,故该文对水体中隐孢子虫的常规检测包括样品收集、浓缩、卵囊分离和染色镜检等步骤,和常用分子生物学方法如多种PCR方法和基于核酸序列的扩增方法等进行综述.
隱孢子蟲(Cryptosporidium spp.)是一類頂複門腸道原蟲,隱孢子蟲病(cryptosporidiosis)是由隱孢子蟲引起的人獸共患原蟲病.由于隱孢子蟲病可通過水傳播,故該文對水體中隱孢子蟲的常規檢測包括樣品收集、濃縮、卵囊分離和染色鏡檢等步驟,和常用分子生物學方法如多種PCR方法和基于覈痠序列的擴增方法等進行綜述.
은포자충(Cryptosporidium spp.)시일류정복문장도원충,은포자충병(cryptosporidiosis)시유은포자충인기적인수공환원충병.유우은포자충병가통과수전파,고해문대수체중은포자충적상규검측포괄양품수집、농축、란낭분리화염색경검등보취,화상용분자생물학방법여다충PCR방법화기우핵산서렬적확증방법등진행종술.
Cryptosporidium spp.ale Apicomplexan protozoan parasites that are pathogen of zoonotic eryptosporidiosis.Cryptosporidiosis can be transmitted via water.This review summarized the routine detection including sample collection,concentration,oocyst purification and microscopic observation,and molecular de-tection such as various PCR methods and nucleic acid sequence-based amplification for detection of waterborne Cryptosporidium.