中华泌尿外科杂志
中華泌尿外科雜誌
중화비뇨외과잡지
CHINESE JOURNAL OF UROLOGY
2011年
6期
368-372
,共5页
邹霜梅%刘宇%罗巍%韩迺珺%薛丽燕%温芃%高燕宁
鄒霜梅%劉宇%囉巍%韓迺珺%薛麗燕%溫芃%高燕寧
추상매%류우%라외%한내군%설려연%온봉%고연저
肾肿瘤%基因,DLK1%生物学标记%病理学,临床
腎腫瘤%基因,DLK1%生物學標記%病理學,臨床
신종류%기인,DLK1%생물학표기%병이학,림상
Kidney neoplasmas%Gene,DLK1%Biological markers%Pathology,clinical
目的 探讨不同组织类型的肾细胞癌组织中DLK1蛋白表达与肾癌临床病理特征及转移的关系.方法 采用免疫组织化学方法,检测94例肾原发透明细胞癌、76例乳头状肾细胞癌、45例嫌色细胞癌、71例透明细胞癌远处转移灶、24例透明细胞癌淋巴结转移灶及18例正常肾组织标本中DLK1蛋白表达情况,分析其与临床病理特征及转移的相关性.结果 正常肾组织近端及远端肾小管中DLK1蛋白表达均为阳性,透明细胞癌、乳头状肾细胞癌及嫌色细胞癌组织低表达率分别为33.0%(31/94)、27.6%(21/76)及33.3%(15/45),与正常肾组织相比,差异有统计学意义(P＜0.05),3种类型肾癌组织之间DLK1蛋白表达差异无统计学意义(P＞0.05).DLK1蛋白表达水平与透明细胞癌患者性别(男60例,女34例)、年龄(≥55岁50例,＜55岁44例)、病理分期(Ⅰ期41例,Ⅱ期9例,Ⅲ期21例,Ⅳ期23例)及淋巴结转移状态(无转移76例,有转移18例)等无明显相关性(P＞0.05).透明细胞癌原发灶、淋巴结转移灶、远处转移灶肿瘤组织中DLK1蛋白表达差异无统计学意义(P＞0.05).结论 不同类型的肾细胞癌组织中DLK1蛋白表达降低.DLK1蛋白低表达与透明细胞癌的临床病理特征及肿瘤转移无关.
目的 探討不同組織類型的腎細胞癌組織中DLK1蛋白錶達與腎癌臨床病理特徵及轉移的關繫.方法 採用免疫組織化學方法,檢測94例腎原髮透明細胞癌、76例乳頭狀腎細胞癌、45例嫌色細胞癌、71例透明細胞癌遠處轉移竈、24例透明細胞癌淋巴結轉移竈及18例正常腎組織標本中DLK1蛋白錶達情況,分析其與臨床病理特徵及轉移的相關性.結果 正常腎組織近耑及遠耑腎小管中DLK1蛋白錶達均為暘性,透明細胞癌、乳頭狀腎細胞癌及嫌色細胞癌組織低錶達率分彆為33.0%(31/94)、27.6%(21/76)及33.3%(15/45),與正常腎組織相比,差異有統計學意義(P＜0.05),3種類型腎癌組織之間DLK1蛋白錶達差異無統計學意義(P＞0.05).DLK1蛋白錶達水平與透明細胞癌患者性彆(男60例,女34例)、年齡(≥55歲50例,＜55歲44例)、病理分期(Ⅰ期41例,Ⅱ期9例,Ⅲ期21例,Ⅳ期23例)及淋巴結轉移狀態(無轉移76例,有轉移18例)等無明顯相關性(P＞0.05).透明細胞癌原髮竈、淋巴結轉移竈、遠處轉移竈腫瘤組織中DLK1蛋白錶達差異無統計學意義(P＞0.05).結論 不同類型的腎細胞癌組織中DLK1蛋白錶達降低.DLK1蛋白低錶達與透明細胞癌的臨床病理特徵及腫瘤轉移無關.
목적 탐토불동조직류형적신세포암조직중DLK1단백표체여신암림상병리특정급전이적관계.방법 채용면역조직화학방법,검측94례신원발투명세포암、76례유두상신세포암、45례혐색세포암、71례투명세포암원처전이조、24례투명세포암림파결전이조급18례정상신조직표본중DLK1단백표체정황,분석기여림상병리특정급전이적상관성.결과 정상신조직근단급원단신소관중DLK1단백표체균위양성,투명세포암、유두상신세포암급혐색세포암조직저표체솔분별위33.0%(31/94)、27.6%(21/76)급33.3%(15/45),여정상신조직상비,차이유통계학의의(P＜0.05),3충류형신암조직지간DLK1단백표체차이무통계학의의(P＞0.05).DLK1단백표체수평여투명세포암환자성별(남60례,녀34례)、년령(≥55세50례,＜55세44례)、병리분기(Ⅰ기41례,Ⅱ기9례,Ⅲ기21례,Ⅳ기23례)급림파결전이상태(무전이76례,유전이18례)등무명현상관성(P＞0.05).투명세포암원발조、림파결전이조、원처전이조종류조직중DLK1단백표체차이무통계학의의(P＞0.05).결론 불동류형적신세포암조직중DLK1단백표체강저.DLK1단백저표체여투명세포암적림상병리특정급종류전이무관.
Objective To identify the expression of DLK1 protein in different types of renal cell carcinomas and its correlations with pathological characteristics and metastasis. Methods Immunohistochemistry analysis was performed to evaluate the expression of DLK1 protein in 94 cases of primary clear cell renal cell carcinoma, 76 cases of papillary renal cell carcinoma, 45 cases of chromophobe renal cell carcinoma, 71 cases of distal metastatic and 24 cases of lymph node metastatic clear cell renal cell carcinoma, as well as 18 cases of normal renal tissue. The correlations of DLK1 protein expression with pathological characteristics were analyzed. Results DLK1 protein was expressed in proximal and distal renal tubular epithelial cells in all the normal renal cases. In contrast, DLK1 protein expression was lower in different types of renal cell carcinoma. The low or negative expression of DLK1 protein in clear cell renal cell carcinoma, papillary renal cell carcinoma and chromophobe renal cell carcinoma was 33.0% (31/94), 27.6% (21/76) and 33.3% (15/45), respectively. Compared to normal renal tissue, DLK1 protein expression was significantly down-regulated in renal cell carcinomas (P＞0.05), whereas there was no significant difference on DLK1 protein expressions among the different types (P＞0.05) of renal cell carcinomas. DLK1 protein expression was not correlated with sex (60 male and 34 female cases), age (≥55, 50 cases and 55, 44 cases), grade (41 cases in grade I, 9 cases in grade II, 21 cases in grade III and 23 cases in grade Ⅳ respectively) and lymph node metastasis (76 cases with and 18 cases without lymph node metastasis) in clear cell renal cell carcinoma (P＞0.05). There was also no significant difference among primary, lymph node and distal metastatic lesions of clear cell carcinoma (P＞0.05). Conclusions DLK1 protein expression is commonly down-regulated in different types of renal cell carcinomas. Down-regulation of DLK1 protein expression is not associated with pathological characteristics and metastasis in clear cell renal cell carcinoma.
