中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2011年
9期
766-769
,共4页
陶绍富%王春锋%丁晶%饶龙华%李济宇
陶紹富%王春鋒%丁晶%饒龍華%李濟宇
도소부%왕춘봉%정정%요룡화%리제우
再灌注损伤%血红素加氧酶-1%肥大细胞
再灌註損傷%血紅素加氧酶-1%肥大細胞
재관주손상%혈홍소가양매-1%비대세포
Reperfusion injury%Heme oxygenase-1%Mast cells
目的 探索HO-1对肝脏缺血再灌注损伤中肥大细胞脱颗粒的影响。方法 将20只SD大鼠随机分成4组：假手术组（Sham组），缺血再灌注损伤组（I/RI组），HO-1诱导剂钴原卟啉组(CoPP组，术前24h给予CoPP，5 mg/kg)及HO-1抑制剂锌原卟啉组(ZnPP组，术前24h给予ZnPP，20 mg/kg)。建立大鼠缺血再灌注损伤模型，各组于再灌注后2h收集标本。RT-PCR检测肝脏组织HO-1 mRNA表达，Western blot检测肝脏组织HO-1蛋白表达；测定血清中ALT、AST水平；肝脏组织甲苯胺蓝染色检测肥大细胞脱颗粒数量，HE染色评价肝脏组织损伤情况。结果 与Sham组相比，I/RI组、CoPP组、ZnPP组大鼠组织HO-1 RNA和蛋白表达增加，血清ALT、AST水平升高，肥大细胞脱颗粒数量增多，肝脏细胞损伤加重。CoPP组与I/RI组相比，HO-1 mRNA和蛋白表达增加，血清ALT、AST水平减低，肥大细胞脱颗粒数量减少，肝细胞损伤减轻。ZnPP组与I/RI组相比，HO-1 mRNA和蛋白表达减少，血清ALT、AST水平升高，肥大细胞脱颗粒数量增多、肝细胞损伤严重。组间比较差异具有统计学意义(P＜0.05)。结论 HO-1过表达能减轻肝脏I/RI，其机制可能与抑制肝脏组织中肥大细胞脱颗粒有关。
目的 探索HO-1對肝髒缺血再灌註損傷中肥大細胞脫顆粒的影響。方法 將20隻SD大鼠隨機分成4組：假手術組（Sham組），缺血再灌註損傷組（I/RI組），HO-1誘導劑鈷原卟啉組(CoPP組，術前24h給予CoPP，5 mg/kg)及HO-1抑製劑鋅原卟啉組(ZnPP組，術前24h給予ZnPP，20 mg/kg)。建立大鼠缺血再灌註損傷模型，各組于再灌註後2h收集標本。RT-PCR檢測肝髒組織HO-1 mRNA錶達，Western blot檢測肝髒組織HO-1蛋白錶達；測定血清中ALT、AST水平；肝髒組織甲苯胺藍染色檢測肥大細胞脫顆粒數量，HE染色評價肝髒組織損傷情況。結果 與Sham組相比，I/RI組、CoPP組、ZnPP組大鼠組織HO-1 RNA和蛋白錶達增加，血清ALT、AST水平升高，肥大細胞脫顆粒數量增多，肝髒細胞損傷加重。CoPP組與I/RI組相比，HO-1 mRNA和蛋白錶達增加，血清ALT、AST水平減低，肥大細胞脫顆粒數量減少，肝細胞損傷減輕。ZnPP組與I/RI組相比，HO-1 mRNA和蛋白錶達減少，血清ALT、AST水平升高，肥大細胞脫顆粒數量增多、肝細胞損傷嚴重。組間比較差異具有統計學意義(P＜0.05)。結論 HO-1過錶達能減輕肝髒I/RI，其機製可能與抑製肝髒組織中肥大細胞脫顆粒有關。
목적 탐색HO-1대간장결혈재관주손상중비대세포탈과립적영향。방법 장20지SD대서수궤분성4조：가수술조（Sham조），결혈재관주손상조（I/RI조），HO-1유도제고원계람조(CoPP조，술전24h급여CoPP，5 mg/kg)급HO-1억제제자원계람조(ZnPP조，술전24h급여ZnPP，20 mg/kg)。건립대서결혈재관주손상모형，각조우재관주후2h수집표본。RT-PCR검측간장조직HO-1 mRNA표체，Western blot검측간장조직HO-1단백표체；측정혈청중ALT、AST수평；간장조직갑분알람염색검측비대세포탈과립수량，HE염색평개간장조직손상정황。결과 여Sham조상비，I/RI조、CoPP조、ZnPP조대서조직HO-1 RNA화단백표체증가，혈청ALT、AST수평승고，비대세포탈과립수량증다，간장세포손상가중。CoPP조여I/RI조상비，HO-1 mRNA화단백표체증가，혈청ALT、AST수평감저，비대세포탈과립수량감소，간세포손상감경。ZnPP조여I/RI조상비，HO-1 mRNA화단백표체감소，혈청ALT、AST수평승고，비대세포탈과립수량증다、간세포손상엄중。조간비교차이구유통계학의의(P＜0.05)。결론 HO-1과표체능감경간장I/RI，기궤제가능여억제간장조직중비대세포탈과립유관。
Objective To investigate the effects of HO-1 on mast cells degranulation during liver ischemia/reperfusion injury. Methods SD rats ( n =20) were randomly divided into 4 groups : ( 1 ) sham operation group, (2) ischemia/reperfusion injury group (I/RI group), (3) CoPP group, CoPP was given before 24 h(5 mg/kg), (4) ZnPP group, ZnPP was given before 24 h (20 mg/kg). The rat model of liver ischemia/reperfusion was built. Samples were collected after 2 hours reperfusion. HO-1 mRNA expression was assessed by RT-PCR. The expression of HO-1 protein was assessed by Western blot. Serum ALT, AST levels were determined. The amount of degranulated mast cells were detected by toluidine blue staining. Liver injuries were evaluated by HE staining.Results Compared to sham group, liver HO-1mRNA and protein expression, ALT, AST levels, the number of degranulated mast cells significantly increased in I/RI group, CoPP group and ZnPP group. Moreover, liver injuries were more serious. Compared to I/RI group, there were increased HO-1mRNA and protein expression, reduced ALT, AST levels and the number of degranulated mast cells, also reduced liver injuries were found in CoPP group. Compared to I/RI group, the expression of HO-ImRNA and protein was down-regulated, ALT, AST levels elevated and the number of degranulated mast cells increased accompanying more serious liver injuries in ZnPP group.Conclusions HO-1 overexpression reduces liver ischemia/reperfusion injury, possibly by inhibiting mast cells degranulation in liver tissues.
