中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2009年
5期
497-501
,共5页
郭俊涛%周艺彪%张志杰%刘刚明%依火伍力%王海银%赵根明
郭俊濤%週藝彪%張誌傑%劉剛明%依火伍力%王海銀%趙根明
곽준도%주예표%장지걸%류강명%의화오력%왕해은%조근명
湖北钉螺%微卫星%空间自相关%Moran'sI
湖北釘螺%微衛星%空間自相關%Moran'sI
호북정라%미위성%공간자상관%Moran'sI
Oncomelania hupensis%Microsatellite(simple sequence repeat)%Spatial autocorrelation analysis%Moran's I
目的 采用小尺度空间自相关分析方法对四川省普格县的湖北钉螺自然居群微卫星(SSR)遗传变异的空间结构进行分析,探讨湖北钉螺自然居群遗传变异的空间分布特征.方法 选用5对SSR引物对湖北钉螺基因组DNA进行扩增,选择频率在15%～85%的72个SSR等位基因,运用等样本对频率方法划分14个距离等级分别计算空间自相关系数Moran's I,.结果 5对SSR引物经PCR扩增共得到274个等位基因,SSR等位基因的平均多态信息量高达0.965,表现出很高的遗传多态性.钉螺种群中39个SSR等位基因的遗传变异存在一定程度的空间结构,表现为不同模式的正空间自相关.根据这39个等位基因在14个距离等级上的平均Moran's I值,可以发现随着距离增大正空间自相关性逐渐减小,但未发现表现为负空间自相关的距离等级和等位基因.结论 普格县湖北钉螺种群中SSR遗传变异的空间分布,表现为随着距离增加而减少的正空间自相关性.
目的 採用小呎度空間自相關分析方法對四川省普格縣的湖北釘螺自然居群微衛星(SSR)遺傳變異的空間結構進行分析,探討湖北釘螺自然居群遺傳變異的空間分佈特徵.方法 選用5對SSR引物對湖北釘螺基因組DNA進行擴增,選擇頻率在15%～85%的72箇SSR等位基因,運用等樣本對頻率方法劃分14箇距離等級分彆計算空間自相關繫數Moran's I,.結果 5對SSR引物經PCR擴增共得到274箇等位基因,SSR等位基因的平均多態信息量高達0.965,錶現齣很高的遺傳多態性.釘螺種群中39箇SSR等位基因的遺傳變異存在一定程度的空間結構,錶現為不同模式的正空間自相關.根據這39箇等位基因在14箇距離等級上的平均Moran's I值,可以髮現隨著距離增大正空間自相關性逐漸減小,但未髮現錶現為負空間自相關的距離等級和等位基因.結論 普格縣湖北釘螺種群中SSR遺傳變異的空間分佈,錶現為隨著距離增加而減少的正空間自相關性.
목적 채용소척도공간자상관분석방법대사천성보격현적호북정라자연거군미위성(SSR)유전변이적공간결구진행분석,탐토호북정라자연거군유전변이적공간분포특정.방법 선용5대SSR인물대호북정라기인조DNA진행확증,선택빈솔재15%～85%적72개SSR등위기인,운용등양본대빈솔방법화분14개거리등급분별계산공간자상관계수Moran's I,.결과 5대SSR인물경PCR확증공득도274개등위기인,SSR등위기인적평균다태신식량고체0.965,표현출흔고적유전다태성.정라충군중39개SSR등위기인적유전변이존재일정정도적공간결구,표현위불동모식적정공간자상관.근거저39개등위기인재14개거리등급상적평균Moran's I치,가이발현수착거리증대정공간자상관성축점감소,단미발현표현위부공간자상관적거리등급화등위기인.결론 보격현호북정라충군중SSR유전변이적공간분포,표현위수착거리증가이감소적정공간자상관성.
Objective To analysis the spatial autocorrelation on the small-scale distribution of the genetic variation in the population of Oncomelania hupensis in Puge county, Sichuan province, using simple sequence repeat (SSR) marker. Methods 5 pairs of SSR primer were used to amplify the genomic DNA of Oncomelania hupensis, and the alleles with frequency ranging from 15% to 85% were used to calculate Moran' s I spatial autocorrelation coefficients in 14 distance band based on equal numbers of paired samples. Results A total of 274 alleles were scored by 5 pairs of SSR primer, the average polymorphic information content of the 274 alleles were 0.965 which indicated a high level of genetic diversity. 39 alleles showed different patterns of positive spatial autocorrelation of genetic variation, which was non-random spatial structure. When the distance band increased, the spatial auto-correlativity decreased based on the average Moran' s I value at 14 distance band. The alleles which showed a negative spatial autocorrelation were not found in any distance band. Conclusion The spatial distribution of the genetic variation of SSR showed positive spatial autocorrelation in the population of Oncomelania hupensis, and the spatial auto-correlativity decreased with the increase of distance band.
