CAJ | 학술논문

目的评价不同神经毁损药物对大鼠坐骨神经运动神经功能的影响.方法 SD大鼠35只,随机分为7组(n=5):正常对照组(C组)、阿霉素组(Ad组)、无水乙醇组(Aa组)、8%酚甘油组(Pg1组)、10%酚甘油组(Pg2组)、12%酚甘油组(Pg3组)和庆大霉素组(Ci组).于臀肌间隙显露左侧坐骨神经,在坐骨神经穿入半膜肌和内收肌处注射相应药液各0.2 ml,C组:0.9%生理盐水,Ad组:5 mg/ml阿霉素,Aa组:无水乙醇,Pg1组:8%酚甘油,Pg2组:10%酚甘油,Pg3组:12%酚甘油,Ci组:4000 U/ml庆大霉素.于注药后21 d记录复合肌肉诱发电位波幅,并计算运动神经传导速度.结果各组感觉神经传导速度均为0.与C组比较,其余各组运动神经传导速度和复合肌肉诱发电位波幅均明显降低(P＜0.05);Ad组运动神经传导速度和复合肌肉诱发电位波幅明显高于其它药物组(P＜0.05);Aa组及Pg3组运动神经传导速度和复合肌肉诱发电位波幅明显低于其余各组(P＜0.05).结论与无水乙醇、酚甘油及庆大霉素相比,5%阿霉素对大鼠运动神经功能的影响较小,提示其更适合作为神经毁损药物.
목적 평개불동신경훼손약물대대서좌골신경운동신경공능적영향.방법 SD대서35지,수궤분위7조(n=5):정상대조조(C조)、아매소조(Ad조)、무수을순조(Aa조)、8%분감유조(Pg1조)、10%분감유조(Pg2조)、12%분감유조(Pg3조)화경대매소조(Ci조).우둔기간극현로좌측좌골신경,재좌골신경천입반막기화내수기처주사상응약액각0.2 ml,C조:0.9%생리염수,Ad조:5 mg/ml아매소,Aa조:무수을순,Pg1조:8%분감유,Pg2조:10%분감유,Pg3조:12%분감유,Ci조:4000 U/ml경대매소.우주약후21 d기록복합기육유발전위파폭,병계산운동신경전도속도.결과 각조감각신경전도속도균위0.여C조비교,기여각조운동신경전도속도화복합기육유발전위파폭균명현강저(P＜0.05);Ad조운동신경전도속도화복합기육유발전위파폭명현고우기타약물조(P＜0.05);Aa조급Pg3조운동신경전도속도화복합기육유발전위파폭명현저우기여각조(P＜0.05).결론여무수을순、분감유급경대매소상비,5%아매소대대서운동신경공능적영향교소,제시기경괄합작위신경훼손약물.
Objective To investigate the change in the function of the motor nerve fibers of the sciatic nerve of rat following injection of different neurolytic drugs. Methods Thirty-five SD rats weighing 350-380 g were randomly divided into 7 groups ( n = 5 each): group C received normal saline; group Ad adriamycin 5 mg/ml;group Aa anhydrous alcohol; group Pg1 8% phenol-glycerol; group Pg2 10% phenol-glycerol; group Pg3 12% phenol-glycerol and group Ci cidomycin 4000 U/ml. 0.2 ml of the different neurolytic drugs was injected at the points where the branches of the sciatic nerve entering semi-membranous and adductor magnus muscles. The action potential and conduction velocity of motor and sensory nerve fibers were measured at 21 d after injection. Results The conduction velocity of sensory nerve fibers was 0 in each group. The conduction velocity and action potential of the motor nerve fibers were significantly decreased in group Ad, Aa, Pg1, Pg2, Pg3 and Ci as compared with group C (the control group). The action potential and the conduction velocity were significantly higher in group Ad than in other neurolytic drug groups. The potential and the conduction velocity were the lowest in group Aa and Pg3.The conduction velocity in group Pg1, Pg2, Ci was comparable. Conclusion 5% adriamycin seems to be a better neurolytic drug with less interference with function of motor never fibers.