CAJ | 학술논문

目的探讨复杂核型异常白血病患者的p53基因异常的情况及其临床意义.方法使用间期荧光原位杂交技术(interphase fluorescence in situ hybridisation,I-FISH)分析38例复杂核型异常及24例非复杂核型异常的白血病患者的p53基因异常的情况.结果 38例复杂核型异常的白血病患者p53基因的缺失频率为44.74%(17/38),24例非复杂核型异常的缺失频率为4.16%(1/24),两者之间差异有统计学意义(P<0.01).其中13例伴复杂核型的急性白血病患者完全缓解率15.40%,中位生存时间105天.结论 I-FISH可以快速、准确、灵敏的检测出p53基因的缺失,伴复杂核型的白血病患者p53基因缺失频率较高,完全缓解率低,中位生存时间较短.
목적 탐토복잡핵형이상백혈병환자적p53기인이상적정황급기림상의의.방법 사용간기형광원위잡교기술(interphase fluorescence in situ hybridisation,I-FISH)분석38례복잡핵형이상급24례비복잡핵형이상적백혈병환자적p53기인이상적정황.결과 38례복잡핵형이상적백혈병환자p53기인적결실빈솔위44.74%(17/38),24례비복잡핵형이상적결실빈솔위4.16%(1/24),량자지간차이유통계학의의(P<0.01).기중13례반복잡핵형적급성백혈병환자완전완해솔15.40%,중위생존시간105천.결론 I-FISH가이쾌속、준학、령민적검측출p53기인적결실,반복잡핵형적백혈병환자p53기인결실빈솔교고,완전완해솔저,중위생존시간교단.
Objective To investigate the p53 deletion in leukemia patients with complex chromosomal abnormalities (CCA) and the clinical significance. Methods The p53 deletion status of 38 leukemia cases with CCA and 24 cases without CCA were analyzed by interphase fluorescence in situ hybridization (I-FISH). Results The frequency of p53 deletion in the 38 patients with CCA (44.74%) was significantly higher than that in the 24 cases without CCA (4. 16%) (P<0. 01). The rate of complete remission in 13 cases of acute leukemia with CCA was 15.4%, with median survival time (MST) of 105days. Conclusion I-FISH is a rapid, accurate and sensitive technique for detection of p53 deletion, patients with CCA have a higher p53 deletion and a lower complete remission rate and MST.