CAJ | 학술논문

目的观察人脐带间充质干细胞(hUC-MSCs)兔眼玻璃体腔注射后的生存情况及安全性.方法 27只青紫蓝兔随机分为玻璃体腔注射1、2、4周组,每组9只兔.右眼为hUC-MSCs玻璃体腔注射组(实验组),左眼为培养液玻璃体腔注射组(对照组).注射前后分别采用裂隙灯显微镜进行眼前节检查,间接检眼镜、眼底照相、荧光素眼底血管造影(FFA)进行眼底检查,Tono-pen眼压计测量眼压.体外碳花氰荧光染料CM-Dil荧光标记hUC-MSCs.兔眼玻璃体腔hUC-MSCs注射后4周,共聚焦荧光显微镜下观察hUC-MSCs在体内的存活情况,然后摘除眼球行光学显微镜和透射电子显微镜检查.结果 hUc-MSCs玻璃体腔注射后4周.大量hUC-MSCs仍旧存活于玻璃体腔,结构完整.兔全身情况良好;裂隙灯显微镜检查显示眼前节无明显改变;间接检眼镜和眼底照相检查显示实验眼和对照眼视网膜无明显改变;FFA检查显示注射后1、2、4周视网膜血管无异常荧光和荧光渗漏;注射前后各时间点眼压差异无统计学意义(t=0.125,P>0.05);光学显微镜和透射电子显微镜检查显示兔眼角膜、房角、晶状体、视网膜结构无明显变化.结论 hUC-MSCs在玻璃体腔注射后4周仍存活于体内;hUC-MSCs玻璃体腔注射安全可行.
목적 관찰인제대간충질간세포(hUC-MSCs)토안파리체강주사후적생존정황급안전성.방법 27지청자람토수궤분위파리체강주사1、2、4주조,매조9지토.우안위hUC-MSCs파리체강주사조(실험조),좌안위배양액파리체강주사조(대조조).주사전후분별채용렬극등현미경진행안전절검사,간접검안경、안저조상、형광소안저혈관조영(FFA)진행안저검사,Tono-pen안압계측량안압.체외탄화청형광염료CM-Dil형광표기hUC-MSCs.토안파리체강hUC-MSCs주사후4주,공취초형광현미경하관찰hUC-MSCs재체내적존활정황,연후적제안구행광학현미경화투사전자현미경검사.결과 hUc-MSCs파리체강주사후4주.대량hUC-MSCs잉구존활우파리체강,결구완정.토전신정황량호;렬극등현미경검사현시안전절무명현개변;간접검안경화안저조상검사현시실험안화대조안시망막무명현개변;FFA검사현시주사후1、2、4주시망막혈관무이상형광화형광삼루;주사전후각시간점안압차이무통계학의의(t=0.125,P>0.05);광학현미경화투사전자현미경검사현시토안각막、방각、정상체、시망막결구무명현변화.결론 hUC-MSCs재파리체강주사후4주잉존활우체내;hUC-MSCs파리체강주사안전가행.
Objective To observe the survival of human umbilical cord derived mesenchymal stem cells (hUC-MSCs) after injection into the vitreous of rabbits,and the animal safety under those procedures.Methods Twenty-seven pigmented rabbits were randomly divided into 3 groups (intravitreal injection 1 week group,2 weeks group and 4 weeks group),each with 9 rabbits.For each animal the right eye was the experimental eye receiving hUC-MSCs injection,while the left eye was the control eye receiving cuhure medium.The rabbit eyes were examined by slit-lamp microscope,indirect ophthalmoscopy,fundus photography,fundus fluorescence angiography(FFA)and Tono-pen tonometer before and after injection.hUC-MSCs were labeled by CM-Dil in vitro,and their survival status was measured by eonfocal fluorescence microscopy,light microscope and transmission electron microscope at 4 weeks after injection.Results Four weeks after injection,a large number of the hUC-MSCs were still alive in the vitreous cavity.The overall condition of those rabbits was good.The anterior segment and retina of experimental eyes were normal,without hyperfluorescence,hypofluorescenee and leakage in the retina at 1,2 and 4 weeks after injection.There was no significant difference on lOP before and after injection at different time points (P>0.05),and no obvious changes at cornea,anterior chamber angle,lens,retinal structure by.light microscope and transmission electron microscope examination.Conclusion hUC-MSCs can survive in the rabbit vitreous for four weeks;intravitreal injection of hUC-MSCs was safe and feasible.