CAJ | 학술논문

目的建立成年人与2个月月龄婴儿视网膜组织的双向凝胶电泳图谱,初步分析成年人视网膜与婴儿视网膜蛋白质的表达差异.方法 9例角膜移植后剩余的成年人眼球视网膜组织,2例角膜移植后剩余的2个月月龄婴儿眼球视网膜组织;抽提蛋白质,双向凝胶电泳后考马斯亮蓝染色扫描分析图谱,选取明显差异蛋白点进行质谱鉴定.结果成年人与2个月月龄婴儿视网膜组织的凝胶经考马斯亮蓝染色分别检测到1179和1295个蛋白点,其中1039个蛋白点位置相匹配.5个差异蛋白点的质谱鉴定结果显示,人血清白蛋白、鸟苷酸激酶在成年人的视网膜中高表达;微管蛋白、转醛醇酶和晶体蛋白在2个月月龄婴儿的视网膜中高表达.结论成功建立了人视网膜蛋白质的双向凝胶表达图谱.经质谱鉴定证实了成年人和婴儿的视网膜蛋白质存在表达差异.
목적 건립성년인여2개월월령영인시망막조직적쌍향응효전영도보,초보분석성년인시망막여영인시망막단백질적표체차이.방법 9례각막이식후잉여적성년인안구시망막조직,2례각막이식후잉여적2개월월령영인안구시망막조직;추제단백질,쌍향응효전영후고마사량람염색소묘분석도보,선취명현차이단백점진행질보감정.결과 성년인여2개월월령영인시망막조직적응효경고마사량람염색분별검측도1179화1295개단백점,기중1039개단백점위치상필배.5개차이단백점적질보감정결과현시,인혈청백단백、조감산격매재성년인적시망막중고표체;미관단백、전철순매화정체단백재2개월월령영인적시망막중고표체.결론 성공건립료인시망막단백질적쌍향응효표체도보.경질보감정증실료성년인화영인적시망막단백질존재표체차이.
Objective To identify proteins that have expressed in human eyes from adults and two-month old infants by proteomics approach, so as to build a two-dimensional gel electrophoresis (two-DE) reference map for human retina. The difference of proteomics between the retinas of adults and two-month old infants are also studied. Methods Human retina tissues were collected from donor eyes (nine adults and two infants). Proteins were separated by two-DE. The gels were analyzed by image software. Protein spots were excised from the gels and detected by matrix assisted laser desorption ionization time off light mass spectrometry (MALDI-TOF-MS). Results A total of 1179 spots and 1295 spots were detected respectively on two-DE gels of Coomassie-stained adults and two-month old infants retina, of which 1039 spots were matched in the position. Five spots up-regulated were successfully identified. Human serum albumin and 4 guanylate kinase 1 (GUK1) were identified in adult retina. β2-tubulin, transaldolasel and alpha A-crystallin were identified in infant retina. Conclusion The two-DE reference map for retina proteomics is successfully established. This study provides an evidence of changes in retinal protein levels between adults and infants and biochemical pathways for future studies of human retina development.