中国药学（英文版）
中國藥學（英文版）
중국약학（영문판）
JOURNAL OF CHINESE PHARMACEUTICAL SCIENCES
2007年
1期
9-13
,共5页
硫代寡核苷酸%白蛋白%正电荷密集区%构象变化
硫代寡覈苷痠%白蛋白%正電荷密集區%構象變化
류대과핵감산%백단백%정전하밀집구%구상변화
Phosphorothioate oligodeoxynucleotide%Human serum albumin%Positive potential region%Conformational changes
目的 研究二价阳离子对白蛋白与硫代寡核苷酸结合的影响以及结合硫代寡核苷酸后白蛋白构象的改变.方法 应用表面等离子体共振、圆二色散以及荧光光谱法对白蛋白与硫代寡核苷酸的结合行为进行了表征.结果 (1)随着pH值升高,结合能力降低;(2)锌离子能显著促进白蛋白与硫代寡核苷酸的结合,镍离子也促进结合,但与锌离子相比作用稍弱;(3)与硫代寡核苷酸结合后,白蛋白结构发生变化,产生β-折叠构造.结论 通过实验方法证明硫代寡核苷酸结合于白蛋白的正电荷密集区;表明白蛋白可能介导硫代寡核苷酸的胞吞行为.
目的 研究二價暘離子對白蛋白與硫代寡覈苷痠結閤的影響以及結閤硫代寡覈苷痠後白蛋白構象的改變.方法 應用錶麵等離子體共振、圓二色散以及熒光光譜法對白蛋白與硫代寡覈苷痠的結閤行為進行瞭錶徵.結果 (1)隨著pH值升高,結閤能力降低;(2)鋅離子能顯著促進白蛋白與硫代寡覈苷痠的結閤,鎳離子也促進結閤,但與鋅離子相比作用稍弱;(3)與硫代寡覈苷痠結閤後,白蛋白結構髮生變化,產生β-摺疊構造.結論 通過實驗方法證明硫代寡覈苷痠結閤于白蛋白的正電荷密集區;錶明白蛋白可能介導硫代寡覈苷痠的胞吞行為.
목적 연구이개양리자대백단백여류대과핵감산결합적영향이급결합류대과핵감산후백단백구상적개변.방법 응용표면등리자체공진、원이색산이급형광광보법대백단백여류대과핵감산적결합행위진행료표정.결과 (1)수착pH치승고,결합능력강저;(2)자리자능현저촉진백단백여류대과핵감산적결합,얼리자야촉진결합,단여자리자상비작용초약;(3)여류대과핵감산결합후,백단백결구발생변화,산생β-절첩구조.결론 통과실험방법증명류대과핵감산결합우백단백적정전하밀집구;표명백단백가능개도류대과핵감산적포탄행위.
Aim To study the binding behavior between human serum albumin (HSA) and phosphorothioate oligodeoxynucleotide (PSODN) and the effects of bivalent cations on the interaction. Methods Surface plasma resonance, circular dichroism and fluorescence experiments were conducted. Results ( 1 ) the binding ability was decreased along with the increase of pH; (2) Zn2+and Ni2+ enhanced the interaction between PS-ODN and HSA; (3) Upon PS-ODN binding, the conformation of HSA was changed with an increase of β - sheet.Conclusion The results provide experimental evidences to the hypothesis that PS-ODN binds with HSA in the positive potential region, and histidine residues located in the region play a crucial role in the interaction.
