CAJ | 학술논문

目的探讨肾炎宁对IgA肾病大鼠肾组织巨噬细胞移动抑制因子(macrophage migration inhibitory fac-tor,MIF)的影响.方法将Sprague-Dawley(SD)大鼠随机分为4组:对照组、模型组、西药组(贝那普利+氯沙坦)、中药组(肾炎宁),按各组相应药物剂量灌胃.于第15周末检测各组大鼠24 h尿蛋白量、尿红细胞及IgA免疫荧光检测,并用免疫组织化学法检测MIF蛋白表达水平.结果中药组、西药组大鼠与模型组大鼠24 h尿蛋白量比较差异有统计学意义(P<0.01),且中药组、西药组与模型组比较IgA免疫荧光强度显著下降;中药组与模型组尿红细胞计数比较显著降低,差异有统计学意义(P<0.01),而西药组与模型组尿红细胞计数比较差异无统计学意义(P>0.05);中药组、西药组与模型组比较MIF表达水平显著下降,差异有统计学意义(P<0.01).结论肾炎宁可能通过抑制肾组织MIF表达率发挥对IgA肾病的治疗作用.
목적 탐토신염저대IgA신병대서신조직거서세포이동억제인자(macrophage migration inhibitory fac-tor,MIF)적영향.방법 장Sprague-Dawley(SD)대서수궤분위4조:대조조、모형조、서약조(패나보리+록사탄)、중약조(신염저),안각조상응약물제량관위.우제15주말검측각조대서24 h뇨단백량、뇨홍세포급IgA면역형광검측,병용면역조직화학법검측MIF단백표체수평.결과 중약조、서약조대서여모형조대서24 h뇨단백량비교차이유통계학의의(P<0.01),차중약조、서약조여모형조비교IgA면역형광강도현저하강;중약조여모형조뇨홍세포계수비교현저강저,차이유통계학의의(P<0.01),이서약조여모형조뇨홍세포계수비교차이무통계학의의(P>0.05);중약조、서약조여모형조비교MIF표체수평현저하강,차이유통계학의의(P<0.01).결론 신염저가능통과억제신조직MIF표체솔발휘대IgA신병적치료작용.
Objective To investigate the effect of Shenyanning on the expression of macrophage migration inhibitory factor (M1F) in IgA nephropathy rats. Methods SD rats were randomly divided into 4 groups: control group, model group, western medicine group and Shenyanning group, and afforded with the medicine respectively. At the fifteenth week, 24 h urine protein quant (UPro) , the RBC morphous in urine, IgA immunofluorescence and MIF were detected with relative technique. Results In western medicine group and Shenyanning group, the levels of UPro, IgA immunofluorescence were significantly de-creased in contrast with model groups (P <0. 01) ; Compared with model group, the RBC morphous in urine in Shenyanning group were decreased significantly (P < 0. 01) , while it were not statistically significant in the western medicine group (P > 0. 05) ; In western medicine and Shenyanning groups, the levels of the expression of MIF were significantly decreased in contrast with model group (P<0. 01). Conclusion Shenyanning could treat IgA nephropathy rats effectively by suppressing the expres-sion of macrophage migration inhibitory factor.