CAJ | 학술논문

[目的]研究不同浓度紫杉醇对A549细胞周期、凋亡和4EBP1分子磷酸化的影响.[方法]采用不同浓度紫杉醇处理A549 细胞,建立凋亡和G2/M期阻滞模型;分别用流式细胞术分析细胞凋亡和细胞周期;用免疫印迹方法分析4EBP1的表达与磷酸化.[结果]G2/M期细胞百分率随紫杉醇浓度的增加而增加,但细胞凋亡率随紫杉醇浓度的增加而下降.随G2/M阻滞的增加,4EBP1分子3组丝/苏氨酸位点磷酸化均增强.[结论]紫杉醇在G2/M期上调A549细胞内4EBP1分子多位点的磷酸化.
[목적]연구불동농도자삼순대A549세포주기、조망화4EBP1분자린산화적영향.[방법]채용불동농도자삼순처리A549 세포,건립조망화G2/M기조체모형;분별용류식세포술분석세포조망화세포주기;용면역인적방법분석4EBP1적표체여린산화.[결과]G2/M기세포백분솔수자삼순농도적증가이증가,단세포조망솔수자삼순농도적증가이하강.수G2/M조체적증가,4EBP1분자3조사/소안산위점린산화균증강.[결론]자삼순재G2/M기상조A549세포내4EBP1분자다위점적린산화.
[Objective]The paper aimed to study the effects of different concentrations of paclitaxel on the cell cycle and apoptois of A549 and phosphorylation of 4EBP1. [Method]A cell apoptosis and mitosis ( G2/M stage) retention model was established by treating A549 cell with different concentrations of paclitaxel. Cell apoptosis and cell cycle were determined by flow cytometry.The expression and phosphorylation of 4EBP1 were examined by western-blot. [Result]The percentage of mitosis retention of G2/M stage cells raised with the increase of paclitaxel concentrations,but the percentage of apoptosis decreased. The phosphorylation of 3 groups of serine /threonine were all elevated with the mitosis retention.[Conclusion] Paclitaxel enhanced the phosphorylation of 4EBP1 in A549 cellls at G2/M stage.